Gestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG


Cole, L., Dai, D., Butler, S., Leslie, K. and Kohorn, E. 2006. Gestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG. Gynecologic Oncology. 102 (2), pp. 145-50.
TitleGestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG
AuthorsCole, L., Dai, D., Butler, S., Leslie, K. and Kohorn, E.

Hyperglycosylated hCG (hCG-H) is a glycosylation variant of hCG produced by cytotrophoblast cells at implantation of pregnancy and in choriocarcinoma. We investigated the biological function of hCG-H in invasion in vitro and in vivo and the use of hCG-H antibodies in blocking tumorigenesis and cancer growth in vivo.
Methods and results.
hCG-H accounts for 43% to 100% of total hCG immunoreactivity in the culture fluid of choriocarcinoma cell lines and 100% in primary cultures of pregnancy cytotrophoblast cells. We investigated the action of hCG and hCG-H on isolated cytotrophoblast cell primary cultures and on 3 different lines of choriocarcinoma cells cultured on Matrigel basement membrane inserts (culture models for assessing tumor invasion). The addition of hCG-H to medium significantly promoted invasion of membranes with both pregnancy and cancer cell line sources, while regular hCG had no significant effect.
JEG-3 human choriocarcinoma cells were transplanted subcutaneously into athymic nude mice. Tumors rapidly formed. B152, mouse monoclonal antibody against hCG-H, and non-specific mouse IgG (control) were administered twice weekly once tumors were clearly visible. While a correlation between time and growth was observed with the control group (r2 = 0.97), no correlation was observed with the B152-treated mice (r2 = 0.15). B152 blocked tumor growth (t test, IgG vs. B152, P = 0.003). In a second experiment, antibody B152 or IgG was administered to mice at the time of choriocarcinoma transplantation. B152 significantly inhibited tumorigenesis (t test P = 0.0071).
hCG-H is a critical promoter in human cytotrophoblast and human choriocarcinoma cell invasion in vivo and in vitro, promoting tumor growth and invasion through an autocrine mechanism. hCG-H is a signal for choriocarcinoma cell invasion, making it a biological tumor marker. Antibodies against hCG-H block tumor formation and growth. Human or humanized antibodies against hCG-H may be useful in treating and managing choriocarcinoma and other gestational trophoblastic malignancies.

JournalGynecologic Oncology
Publication dates
PrintAug 2006
Publication process dates
Deposited22 Jun 2009
Output statusPublished
Additional information

PubMed PMID: 16631920.

Digital Object Identifier (DOI)
Permalink -

  • 14
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as