Effect of X and Y box deletions on the development of diabetes in H-2Ea chain transgenic NOD mice

Article


O'Shea, H., Yousaf, N., Altmann, D., Fehervari, Z., Tonks, P., Hetherington, C., Harach, S., Bland, C., Cooke, A. and Lund, T. 2006. Effect of X and Y box deletions on the development of diabetes in H-2Ea chain transgenic NOD mice. Scandinavian Journal of Immunology. 63 (1), pp. 17-25. https://doi.org/10.1111/j.1365-3083.2006.001701.x
TypeArticle
TitleEffect of X and Y box deletions on the development of diabetes in H-2Ea chain transgenic NOD mice
AuthorsO'Shea, H., Yousaf, N., Altmann, D., Fehervari, Z., Tonks, P., Hetherington, C., Harach, S., Bland, C., Cooke, A. and Lund, T.
Abstract

The development of type 1 diabetes in nonobese diabetic (NOD) mice is influenced by major histocompatibility complex (MHC) class II genes. The NOD-E transgenic mouse, which expresses H2-E as a result of the introduction of an Ead gene, is protected from development of type 1 diabetes. While the mechanism of protection remains unclear, the effect has been regarded as a model system for MHC protection from autoimmunity. We have investigated the effect of deletions of the Ea promoter region, which, in turn, affect H2-E expression patterns in transgenic NOD mice. We have constructed transgenic NOD mice where the X (ΔX) and Y (ΔY) boxes of the Ead gene have, respectively, been functionally deleted. Previous reports, using X- or Y-box-deleted H2-E transgenic mice, made by crossing the appropriate transgenes onto the NOD background from C57BL/6 transgenic mice, indicated that promoter mutation abrogated the H2-E-mediated protection seen in NOD-E. The NOD ΔX and NOD ΔY transgenic mice generated in the present study differ in susceptibility to diabetes from wild-type NOD mice. NOD ΔY1 animals are protected from diabetes development, while ΔX mice remain susceptible, albeit to a lesser extent than the parental NOD strain.

PublisherWiley
Scandinavian Society for Immunology
JournalScandinavian Journal of Immunology
ISSN0300-9475
Electronic1365-3083
Publication dates
PrintJan 2006
Publication process dates
Deposited04 Feb 2010
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1365-3083.2006.001701.x
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/8231x

  • 26
    total views
  • 0
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as