Lupus anticoagulant testing: Dilute prothrombin time (dPT)
Book chapter
Moore, G. 2023. Lupus anticoagulant testing: Dilute prothrombin time (dPT). in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.
Chapter title | Lupus anticoagulant testing: Dilute prothrombin time (dPT) |
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Authors | Moore, G. |
Abstract | Lupus anticoagulants (LA) rarely affect routine prothrombin time assays because the high phospholipid (PL) content in thromboplastin reagents tends to overwhelm the antibodies. Dilution of thromboplastin to create a dilute prothrombin time (dPT) screening test renders the assay sensitive to the presence of LA. Technical and diagnostic performances are enhanced if recombinant thromboplastins are employed in place of tissue-derived reagents. Presence of an LA cannot be deduced from an elevated screening test alone since other coagulation disturbances can prolong clotting times. Confirmatory testing with less dilute or undiluted thromboplastin reveals the PL-dependent nature of LA by reducing the clotting time relative to that of the screening test. Where appropriate, such as a known or suspected coagulation factor deficiency, mixing tests are valuable in correcting factor deficiencies and evidencing inhibitory properties of LA, to increase diagnostic specificity. Although LA testing is commonly restricted to dilute Russell's viper venom time and activated partial thromboplastin time, dPT is sensitive to LA unreactive in those assays, and inclusion in routine testing increases detection rates of clinically significant antibodies. |
Keywords | Antiphospholipid Syndrome - diagnosis, Thrombosis, Antiphospholipid antibodies, Lupus Coagulation Inhibitor, Phospholipids, Blood Coagulation Tests, Humans, Antiphospholipid syndrome, Dilute prothrombin time, Phospholipid, Partial Thromboplastin Time, Prothrombin Time, Thromboplastin, Lupus anticoagulant |
Sustainable Development Goals | 3 Good health and well-being |
Middlesex University Theme | Health & Wellbeing |
Book title | Hemostasis and Thrombosis: Methods and Protocols |
Editors | Favaloro, E. and Gosselin, R. |
Publisher | Humana |
Place of publication | New York, NY |
Series | Methods in Molecular Biology (Clifton, N.J.) |
ISBN | |
Hardcover | 978-1-0716-3174-4 |
Electronic | 978-1-0716-3175-1 |
ISSN | 1940-6029 |
Publication dates | |
Online | 20 May 2023 |
20 May 2023 | |
Publication process dates | |
Deposited | 01 Jun 2023 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1007/978-1-0716-3175-1_17 |
Language | English |
https://repository.mdx.ac.uk/item/8q65w
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