ADAMTS13 activity: Screening test protocol

Book chapter


Moore, G., Griffiths, M. and Binder, N. 2023. ADAMTS13 activity: Screening test protocol. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.
Chapter titleADAMTS13 activity: Screening test protocol
AuthorsMoore, G., Griffiths, M. and Binder, N.
Abstract

Accurate estimation of ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif, member 13) activity level is necessary for diagnosis and management of thrombotic microangiopathies (TMA). In particular, it permits distinction between thrombotic thrombocytopenic purpura (TTP) and other TMAs, prompting disorder appropriate treatment. Manual and automated quantitative assays of ADAMTS13 activity are commercially available, some providing results within less than an hour, but they require specialist equipment and personnel and tend to only be available in specialized diagnostic facilities. Technoscreen ADAMTS13 Activity is a rapid, commercially available, semiquantitative screening test employing flow-through technology and an ELISA activity assay principle. It is a simple to perform screening tool, not requiring specialist equipment or personnel. The colored end point is compared to a reference color chart containing four color intensity indicators corresponding to ADAMTS13 activity levels of 0, 0.1, 0.4, or 0.8 IU/mL. Reduced levels detected in the screening test should be confirmed by quantitative assay. The assay lends itself to use in nonspecialized laboratories, remote, and point-of-care settings.

KeywordsADAMTS13 Protein, ADAM Proteins, Diagnosis, Differential, Flow-through assay, ADAMTS13, Purpura, Thrombotic Thrombocytopenic - diagnosis - therapy, Humans, Rapid assay, Screening test, Enzyme-Linked Immunosorbent Assay, Thrombotic thrombocytopenic purpura, Thrombotic microangiopathy
Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
LanguageEnglish
Book titleHemostasis and Thrombosis: Methods and Protocols
EditorsFavaloro, E. and Gosselin, R.
PublisherHumana
Place of publicationNew York, NY
SeriesMethods in Molecular Biology (Clifton, N.J.)
ISBN
Hardcover978-1-0716-3174-4
Electronic978-1-0716-3175-1
ISSN1940-6029
Publication dates
Online20 May 2023
Print20 May 2023
Publication process dates
Deposited01 Jun 2023
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-3175-1_34
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