ADAMTS13 activity measurement by ELISA and fluorescence resonance energy transfer assay

Book chapter


Moore, G., Llusa, M., Griffiths, M. and Binder, N. 2023. ADAMTS13 activity measurement by ELISA and fluorescence resonance energy transfer assay. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.
Chapter titleADAMTS13 activity measurement by ELISA and fluorescence resonance energy transfer assay
AuthorsMoore, G., Llusa, M., Griffiths, M. and Binder, N.
Abstract

Accurate estimation of ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif, member 13) activity level is crucial in the diagnostic setting of differentiation between thrombotic thrombocytopenic purpura (TTP) and other thrombotic microangiopathies. The original assays were too cumbersome and time-consuming for use in the acute situation, and treatment was often based on clinical findings alone, with confirmatory laboratory assays following days or weeks later. Rapid assays are now available that can generate results fast enough to impact on immediate diagnosis and management. Assays based on fluorescence resonance energy transfer (FRET) or chemiluminescence principles can generate results in less than an hour, although they require specific analytical platforms. Enzyme-linked immunosorbent assays (ELISA) can generate results in about 4 h, but do not require specialized equipment beyond ELISA plate readers that are in regular use in many laboratories. The present chapter describes principles, performance, and practical aspects of an ELISA and a FRET assay, for quantitative measurement of ADAMTS13 activity in plasma.

KeywordsADAMTS13 Protein, ADAM Proteins, Fluorescence Resonance Energy Transfer - methods, ADAMTS13, Purpura, Thrombotic Thrombocytopenic - diagnosis - therapy, Humans, Rapid assay, ELISA, Fluorescence resonance energy transfer assay, Enzyme-Linked Immunosorbent Assay, Thrombotic thrombocytopenic purpura, Thrombotic microangiopathy
Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
LanguageEnglish
Book titleHemostasis and Thrombosis: Methods and Protocols
EditorsFavaloro, E. and Gosselin, R.
PublisherHumana
Place of publicationNew York, NY
SeriesMethods in Molecular Biology (Clifton, N.J.)
ISBN
Hardcover978-1-0716-3174-4
Electronic978-1-0716-3175-1
ISSN1940-6029
Publication dates
Online20 May 2023
Print20 May 2023
Publication process dates
Deposited01 Jun 2023
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-3175-1_35
Permalink -

https://repository.mdx.ac.uk/item/8q65y

  • 30
    total views
  • 0
    total downloads
  • 4
    views this month
  • 0
    downloads this month

Export as