Genetic regulation of mouse glycosylphosphatidylinositol-phospholipase D
Article
Flores-Borja, F., Kieszkievicz, J., Church, V., Francis-West, P., Schofield, J., Rademacher, T. and Lund, T. 2004. Genetic regulation of mouse glycosylphosphatidylinositol-phospholipase D. Biochimie. 86 (4-5), pp. 275-282. https://doi.org/10.1016/j.biochi.2004.04.003
| Type | Article |
|---|---|
| Title | Genetic regulation of mouse glycosylphosphatidylinositol-phospholipase D |
| Authors | Flores-Borja, F., Kieszkievicz, J., Church, V., Francis-West, P., Schofield, J., Rademacher, T. and Lund, T. |
| Abstract | Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequence that differs from that previously isolated from a mouse glucagonoma cell library. Using a highly specific and very sensitive RNase protection assay, we found that the GPI-PLD expressed in adult/post-natal brain, antrum and insulin-producing cells is identical to that isolated from liver. The expression of mouse GPI-PLD in liver shows a complex genetic regulation with a mouse strain-specific variation. In addition, GPI-PLD mRNA levels were higher in 4-week old animals compared to older animals, and the GPI-PLD mRNA levels increased in mice that developed insulin dependent type 1 diabetes spontaneously. This suggests that the expression of liver GPI-PLD in mice is highly regulated. |
| Publisher | Elsevier |
| Journal | Biochimie |
| ISSN | 0300-9084 |
| Publication dates | |
| Apr 2004 | |
| Publication process dates | |
| Deposited | 12 Feb 2010 |
| Output status | Published |
| Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biochi.2004.04.003 |
| Language | English |
https://repository.mdx.ac.uk/item/82316
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