Transactivation of the human papilloma virus 16 octamer motif by the octamer binding protein Oct-2 requires both the N and C terminal activation domains

Article


Morris, P., Ring, C., Lillycrop, K. and Latchman, D. 1993. Transactivation of the human papilloma virus 16 octamer motif by the octamer binding protein Oct-2 requires both the N and C terminal activation domains. Nucleic Acids Research. 21 (19), pp. 4506-10.
TypeArticle
TitleTransactivation of the human papilloma virus 16 octamer motif by the octamer binding protein Oct-2 requires both the N and C terminal activation domains
AuthorsMorris, P., Ring, C., Lillycrop, K. and Latchman, D.
Abstract

The upstream regulatory region (URR) of the human papillomaviruses HPV16 and 18 contains a sequence with a seven out of eight base match to the consensus binding site for octamer binding transcription factors. This motif acts as a target for repression by the Oct-1 transcription factor and therefore inhibits promoter activity in non-cervical cells expressing only Oct-1. In contrast the HPV octamer motif activates promoter activity in cervical cells. Here we show that cervical cells express the activating form of the Oct-2 transcription factor, Oct 2.1 and that this factor can transactivate promoter activity via the HPV16 octamer. This effect is dependent upon both the N and C-terminal activation domains of Oct-2. The expression of specific octamer binding proteins such as Oct-2 in cervical cells thus allows the HPV16 motif to produce opposite effects on gene expression in cervical and non-cervical cells suggesting that it may play a role in the cervical specificity of URR driven gene expression.

LanguageEnglish
PublisherOxford U. P.
JournalNucleic Acids Research
ISSN0305-1048
Publication dates
PrintSep 1993
Publication process dates
Deposited03 Dec 2009
Output statusPublished
Web address (URL)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC311182/pdf/nar00068-0084.pdf
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