Ectodomain shedding of the neural recognition molecule CHL1 by the metalloprotease-disintegrin ADAM8 promotes neurite outgrowth and suppresses neuronal cell death

Article


Naus, S., Richter, M., Wildeboer, D., Moss, M., Schachner, M. and Bartsch, J. 2004. Ectodomain shedding of the neural recognition molecule CHL1 by the metalloprotease-disintegrin ADAM8 promotes neurite outgrowth and suppresses neuronal cell death. Journal of Biological Chemistry. 279 (16), pp. 16083-16090. https://doi.org/10.1074/jbc.M400560200
TypeArticle
TitleEctodomain shedding of the neural recognition molecule CHL1 by the metalloprotease-disintegrin ADAM8 promotes neurite outgrowth and suppresses neuronal cell death
AuthorsNaus, S., Richter, M., Wildeboer, D., Moss, M., Schachner, M. and Bartsch, J.
Abstract

The neural cell adhesion molecule “close homologue of L1,” termed CHL1, has functional importance in the nervous system. CHL1 is expressed as a transmembrane protein of 185 kDa, and ectodomain shedding releases soluble fragments relevant for its physiological function. Here we describe that ADAM8, a member of the family of metalloprotease disintegrins cleaves a CHL1-Fc fusion protein in vitro at two sites corresponding to release of the extracellular domain of CHL1 in fibronectin (FN) domains II (125 kDa) and V (165 kDa), inhibited by batimastat (BB-94). Cleavage of CHL1-Fc in the 125-kDa fragment was not detectable under non-reducing conditions arguing that cleavage resulting in the 165-kDa fragment is more relevant in releasing soluble CHL1 in vivo. In cells transfected with full-length ADAM8, membrane proximal cleavage of CHL1 was similar and not stimulated by phorbol ester 12-O-tetradecanoylphorbol-13-acetate and pervanadate. No cleavage of CHL1 was observed in cells expressing either inactive ADAM8 with a Glu330 to Gln exchange (EQ-A8), or active ADAM10 and ADAM17. Consequently, processing of CHL1 was hardly detectable in brain extracts of ADAM8-deficient mice but enhanced in a neurodegenerative mouse mutant. CHL1 processed by ADAM8 in supernatants of COS-7 cells and in co-culture with cerebellar granule neurons was very potent in stimulating neurite outgrowth and suppressing neuronal cell death, not observed in cells co-transfected with CHL1/EQ-A8, CHL1/ADAM10, or CHL1/ADAM17. Taken together, we propose that ADAM8 plays an important role in physiological and pathological cell interactions by a specific release of functional CHL1 from the cell surface.

Research GroupBiophysics and Bioengineering group
PublisherAmerican Society for Biochemistry and Molecular Biology
JournalJournal of Biological Chemistry
ISSN0021-9258
Publication dates
PrintApr 2004
Publication process dates
Deposited02 Mar 2012
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1074/jbc.M400560200
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/83894

  • 50
    total views
  • 0
    total downloads
  • 3
    views this month
  • 0
    downloads this month

Export as

Related outputs

Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year
Hassard, F., Vu, M., Rahimzadeh, S., Castro-Gutierrez, V., Stanton, I., Burczynska, B., Wildeboer, D., Baio, G., Brown, M., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Majeed, A., Priest, S., Denise, H., Khalifa, M., Bassano, I., Wade, M., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2023. Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year. PLoS ONE. 18 (5). https://doi.org/10.1371/journal.pone.0286259
Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach
Castro-Gutierrez, V., Hassard, F., Vu, M., Leitao, R., Burczynska, B., Wildeboer, D., Stanton, I., Rahimzadeh, S., Baio, G., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Kwiatkowska, R., Majeed, A., Priest, S., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2022. Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach. PLoS ONE. 17 (6). https://doi.org/10.1371/journal.pone.0270168
Characterization of the complete mitochondrial genome of Diplostomum baeri
Landeryou, T., Ropiquet, A., Kett, S., Wildeboer, D. and Lawton, S. 2020. Characterization of the complete mitochondrial genome of Diplostomum baeri. Parasitology International. 79. https://doi.org/10.1016/j.parint.2020.102166
Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site
Chan, W., Wildeboer, D., Garelick, H. and Purchase, D. 2018. Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site. Extremophiles. 22 (5), pp. 795-809. https://doi.org/10.1007/s00792-018-1039-2
Metal water-sediment interactions and impacts on an urban ecosystem
Lundy, L., Alves, L., Revitt, D. and Wildeboer, D. 2017. Metal water-sediment interactions and impacts on an urban ecosystem. 14th IWA/IAHR International Conference on Urban Drainage. Prague, Czech Republic 10 - 15 Sep 2017 pp. 148-156
Metal water-sediment interactions and impacts on an urban ecosystem
Lundy, L., Alves, L., Revitt, D. and Wildeboer, D. 2017. Metal water-sediment interactions and impacts on an urban ecosystem. International Journal of Environmental Research and Public Health. 14 (7), pp. 1-12. https://doi.org/10.3390/ijerph14070722
A proteomic study on the responses to arsenate stress by an acidophilic fungal strain Acidomyces acidophilus WKC1
Chan, W., Wildeboer, D., Garelick, H. and Purchase, D. 2016. A proteomic study on the responses to arsenate stress by an acidophilic fungal strain Acidomyces acidophilus WKC1. Biotechnology World Convention. Sao Paulo, Brazil 15 - 17 Aug 2016 OMICS International. pp. 35-35 https://doi.org/10.4172/2155-952X.C1.058
Environmental waters and E. coli as a marker, including pathogenic and resistant strains
Price, R. and Wildeboer, D. 2017. Environmental waters and E. coli as a marker, including pathogenic and resistant strains. in: Samie, A. (ed.) Escherichia coli - Recent Advances on Physiology, Pathogenesis and Biotechnological Applications InTech.
Mycoremediation of heavy metal/metalloid-contaminated soil: current understanding and future prospects
Chan, W., Wildeboer, D., Garelick, H. and Purchase, D. 2016. Mycoremediation of heavy metal/metalloid-contaminated soil: current understanding and future prospects. in: Purchase, D. (ed.) Fungal Applications in Sustainable Environmental Biotechnology Cham, Switzerland Springer. pp. 249-272
Methods of analysis for bacterial contamination in environmental waters
Price, R. and Wildeboer, D. 2015. Methods of analysis for bacterial contamination in environmental waters. in: McCoy, G. (ed.) Coliforms: occurrence, detection methods and environmental impact Nova Science Publishers.
Investigating arsenic resistance in fungi from tin-mining soil and the possible interaction between arsenic and tin/antimony
Chan, W., Wildeboer, D., Garelick, H. and Purchase, D. 2014. Investigating arsenic resistance in fungi from tin-mining soil and the possible interaction between arsenic and tin/antimony. 10th International Mycological Congress. Bangkok, Thailand 03 - 08 Aug 2014
Tumor Necrosis Factor-α (TNF-α) regulates shedding of TNF-α receptor 1 by the metalloprotease-disintegrin ADAM8: evidence for a protease-regulated feedback loop in neuroprotection
Bartsch, J., Wildeboer, D., Koller, G., Naus, S., Rittger, A., Moss, M., Minai, Y. and Jockusch, H. 2010. Tumor Necrosis Factor-α (TNF-α) regulates shedding of TNF-α receptor 1 by the metalloprotease-disintegrin ADAM8: evidence for a protease-regulated feedback loop in neuroprotection. Journal of Neuroscience. 30 (36), pp. 12210-12218. https://doi.org/10.1523/JNEUROSCI.1520-10.2010
ADAM8/MS2/CD156a: a metalloprotease-disintegrin involved in immune responses
Bartsch, J., Naus, S., Rittger, A., Schlomann, U. and Wildeboer, D. 2005. ADAM8/MS2/CD156a: a metalloprotease-disintegrin involved in immune responses. in: Hooper, N. and Lendeckel, U. (ed.) The ADAM family of Proteases Dordrecht, Netherlands Springer.
The metalloprotease disintegrin ADAM8. Processing by autocatalysis is required for proteolytic activity and cell adhesion
Schlomann, U., Wildeboer, D., Webster, A., Antropova, O., Zeuschner, D., Knight, C., Docherty, A., Lambert, M., Skelton, L., Jockusch, H. and Bartsch, J. 2002. The metalloprotease disintegrin ADAM8. Processing by autocatalysis is required for proteolytic activity and cell adhesion. Journal of Biological Chemistry. 277 (50), pp. 48210-48219. https://doi.org/10.1074/jbc.M203355200
Escherichia coli contamination of the river Thames in different seasons and weather conditions
Amirat, L., Wildeboer, D., Abuknesha, R. and Price, R. 2012. Escherichia coli contamination of the river Thames in different seasons and weather conditions. Water and Environment Journal. 26 (4), pp. 482-489. https://doi.org/10.1111/j.1747-6593.2012.00308.x
Specific protease activity indicates the degree of Pseudomonas aeruginosa infection in chronic infected wounds
Wildeboer, D., Hill, K., Jeganathan, F., Williams, D., Riddell, A., Price, P., Thomas, D., Stephens, P., Abuknesha, R. and Price, R. 2012. Specific protease activity indicates the degree of Pseudomonas aeruginosa infection in chronic infected wounds. European Journal of Clinical Microbiology & Infectious Diseases. 31 (9), pp. 2183-2189. https://doi.org/10.1007/s10096-012-1553-6
Optimisation of the detection of bacterial proteases using adsorbed immunoglobulins as universal substrates
Abuknesha, R., Jeganathan, F., Wildeboer, D. and Price, R. 2010. Optimisation of the detection of bacterial proteases using adsorbed immunoglobulins as universal substrates. Talanta. 81 (4-5), pp. 1237-1244. https://doi.org/10.1016/j.talanta.2010.02.015
Detection of proteases using an immunochemical method with haptenylated–gelatin as a solid-phase substrate
Abuknesha, R., Jeganathan, F., DeGroot, R., Wildeboer, D. and Price, R. 2010. Detection of proteases using an immunochemical method with haptenylated–gelatin as a solid-phase substrate. Analytical and Bioanalytical Chemistry. 396 (7), pp. 2547-2558. https://doi.org/10.1007/s00216-010-3540-z
Rapid detection of Escherichia coli in water using a hand-held fluorescence detector
Wildeboer, D., Amirat, L., Price, R. and Abuknesha, R. 2010. Rapid detection of Escherichia coli in water using a hand-held fluorescence detector. Water Research. 44 (8), pp. 2621-2628. https://doi.org/10.1016/j.watres.2010.01.020
Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8.
Naus, S., Reipschläger, S., Wildeboer, D., Lichtenthaler, S., Mitterreiter, S., Guan, Z., Moss, M. and Bartsch, J. 2006. Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8. Biological Chemistry. 387 (3), pp. 337-346. https://doi.org/10.1515/BC.2006.045
Metalloproteinase disintegrins ADAM8 and ADAM19 are highly regulated in human primary brain tumors and their expression levels and activities are associated with invasiveness.
Wildeboer, D., Naus, S., Sang, Q., Bartsch, J. and Pagenstecher, A. 2006. Metalloproteinase disintegrins ADAM8 and ADAM19 are highly regulated in human primary brain tumors and their expression levels and activities are associated with invasiveness. Journal of Neuropathology and Experimental Neurology. 65 (5), pp. 516-527.
Screening of herbal constituents for aromatase inhibitory activity
Paoletta, S., Steventon, G., Wildeboer, D., Ehrman, T., Hylands, P. and Barlow, D. 2008. Screening of herbal constituents for aromatase inhibitory activity. Bioorganic & Medicinal Chemistry. 16 (18), pp. 8466-8470. https://doi.org/10.1016/j.bmc.2008.08.034
Use of antibody–hapten complexes attached to optical sensor surfaces as a substrate for proteases: real-time biosensing of protease activity
Wildeboer, D., Jiang, P., Price, R., Yu, S., Jeganathan, F. and Abuknesha, R. 2010. Use of antibody–hapten complexes attached to optical sensor surfaces as a substrate for proteases: real-time biosensing of protease activity. Talanta. 81 (1-2), pp. 68-75. https://doi.org/10.1016/j.talanta.2009.11.036
Characterization of bacterial proteases with a panel of fluorescent peptide substrates
Wildeboer, D., Jeganathan, F., Price, R. and Abuknesha, R. 2009. Characterization of bacterial proteases with a panel of fluorescent peptide substrates. Analytical Biochemistry. 384 (2), pp. 321-328. https://doi.org/10.1016/j.ab.2008.10.004
The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events
Moss, M., Bomar, M., Liu, Q., Sage, H., Dempsey, P., Lenhart, P., Gillispie, P., Stoeck, A., Wildeboer, D., Bartsch, J., Palmisano, R. and Zhou, P. 2007. The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events. Journal of Biological Chemistry. 282 (49), pp. 35712-35721. https://doi.org/10.1074/jbc.M703231200