Dr Nick Kassouf

Dr Nick Kassouf
NameDr Nick Kassouf
Job titleTechnical Tutor Biomedical Science
Research institute
Primary appointmentScience & Engineering Labs
Email addressN.Kassouf@mdx.ac.uk
ORCIDhttps://orcid.org/0000-0003-0519-1929
Contact categoryResearcher

Biography

Biography

My role as Technical Tutor in Biomedical Science primarily encompasses teaching on BSc and MSc programmes within a laboratory setting. My students learn diagnostic tools to diagnose disorders such as blood disorders as well as research tools such as qPCR to detect the presence of HPV viruses in cancer cell lines. A wide range of cell and molecular biology techniques are taught and utilised within our extensive range of practicals such as RT-PCR, Western Blotting, Immunocytochemistry, flow cytometry, ELISA and Spectrophotometry.

Teaching

I currently teach on the Biomedical Science BSc and MSc programme within the Middlesex University. I supervise BSc dissertation project students on their Dissertation module in their final year. I am an Academic Advisor for 2nd and 3rd year Biomedical Science students.

Employment

Technical Tutor in Biomedical Science
Middlesex University
03 Sept 2015

Education and qualifications

PhD
01 Nov 2005
Queen Mary, University of London

Research outputs

Betulinic Acid–Doxorubicin-Drug combination induced apoptotic death via ROS stimulation in a relapsed AML MOLM-13 cell model

Vu, M., Kassouf, N. and Appiah, S. 2021. Betulinic Acid–Doxorubicin-Drug combination induced apoptotic death via ROS stimulation in a relapsed AML MOLM-13 cell model. Antioxidants. 10 (9). https://doi.org/10.3390/antiox10091456

Doxorubicin selectively induces apoptosis through the inhibition of a novel isoform of Bcl‑2 in acute myeloid leukaemia MOLM‑13 cells with reduced Beclin 1 expression

Vu, M., Kassouf, N., Ofili, R., Lund, T., Bell, C. and Appiah, S. 2020. Doxorubicin selectively induces apoptosis through the inhibition of a novel isoform of Bcl‑2 in acute myeloid leukaemia MOLM‑13 cells with reduced Beclin 1 expression. International Journal of Oncology. 57 (1), pp. 113-121. https://doi.org/10.3892/ijo.2020.5052

Betulinic acid-doxorubicin drug combination synergistically supresses cell viability and enhances apoptotic death in acute myeloid leukaemia cell lines by increasing Bax/Bcl-2 ratio

Vu, M., Kassouf, N., Bell, C. and Appiah, S. 2018. Betulinic acid-doxorubicin drug combination synergistically supresses cell viability and enhances apoptotic death in acute myeloid leukaemia cell lines by increasing Bax/Bcl-2 ratio. NCRI Cancer Conference (2018). Glasgow, Scotland 04 - 06 Nov 2018

UVA-induced carbon-centered radicals in lightly pigmented cells detected using ESR spectroscopy

Kassouf, N., Kay, C., Volkov, A., Chiang, S., Birch-Machin, M., El-Khamisy, S. and Haywood, R. 2018. UVA-induced carbon-centered radicals in lightly pigmented cells detected using ESR spectroscopy. Free Radical Biology and Medicine. 126, pp. 153-165. https://doi.org/10.1016/j.freeradbiomed.2018.07.019

Mitochondrial protein-linked DNA breaks perturb mitochondrial gene transcription and trigger free radical-induced DNA damage

Chiang, S., Meagher, M., Kassouf, N., Hafezparast, M., McKinnon, P., Haywood, R. and El-Khamisy, S. 2017. Mitochondrial protein-linked DNA breaks perturb mitochondrial gene transcription and trigger free radical-induced DNA damage. Science Advances. 3 (4). https://doi.org/10.1126/sciadv.1602506

Evaluation of absorbent materials for use as ad hoc dry decontaminants during mass casualty incidents as part of the UK's Initial Operational Response (IOR)

Kassouf, N., Syed, S., Larner, J., Amlot, R. and Chilcott, R. 2017. Evaluation of absorbent materials for use as ad hoc dry decontaminants during mass casualty incidents as part of the UK's Initial Operational Response (IOR). PLoS ONE. 12 (2). https://doi.org/10.1371/journal.pone.0170966

Phosphatidylinositol-3,4,5-trisphosphate stimulates Ca2+ elevation and Akt phosphorylation to constitute a major mechanism of thromboxane A2 formation in human platelets

Kassouf, N., Ambily, A., Watson, S., Hassock, S., Authi, H., Srivastava, S., Watson, S. and Authi, K. 2015. Phosphatidylinositol-3,4,5-trisphosphate stimulates Ca2+ elevation and Akt phosphorylation to constitute a major mechanism of thromboxane A2 formation in human platelets. Cellular Signalling. 27 (7), pp. 1488-1498. https://doi.org/10.1016/j.cellsig.2015.03.008

The role of plasma membrane STIM1 and Ca2+entry in platelet aggregation. STIM1 binds to novel proteins in human platelets

Ambily, A., Kaiser, W., Pierro, C., Chamberlain, E., Li, Z., Jones, C., Kassouf, N., Gibbins, J. and Authi, K. 2014. The role of plasma membrane STIM1 and Ca2+entry in platelet aggregation. STIM1 binds to novel proteins in human platelets. Cellular Signalling. 26 (3), pp. 502-511. https://doi.org/10.1016/j.cellsig.2013.11.025

Intensity-dependent direct solar radiation- and UVA-induced radical damage to human skin and DNA, lipids and proteins

Haywood, R., Andrady, C., Kassouf, N. and Sheppard, N. 2011. Intensity-dependent direct solar radiation- and UVA-induced radical damage to human skin and DNA, lipids and proteins. Photochemistry and Photobiology. 87 (1), pp. 117-130. https://doi.org/10.1111/j.1751-1097.2010.00850.x

Isolation of ORCTL3 in a novel genetic screen for tumor-specific apoptosis inducers

Irshad, S., Mahul-Mellier, A., Kassouf, N., Lemarie, A. and Grimm, S. 2009. Isolation of ORCTL3 in a novel genetic screen for tumor-specific apoptosis inducers. Cell Death and Differentiation. 16 (6), pp. 890-898. https://doi.org/10.1038/cdd.2009.21

Oral cancer cell lines can use multiple ligands, including Fas-L, TRAIL and TNF-a, to induce apoptosis in Jurkat T cells: Possible mechanisms for immune escape by head and neck cancers

Kassouf, N. and Thornhill, M. 2008. Oral cancer cell lines can use multiple ligands, including Fas-L, TRAIL and TNF-a, to induce apoptosis in Jurkat T cells: Possible mechanisms for immune escape by head and neck cancers. Oral Oncology. 44 (7), pp. 672-682. https://doi.org/10.1016/j.oraloncology.2007.08.013
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