The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis
Article
Chen, D., Janganan, T.K., Chen, G., Marques, E.R., Kress, M.R., Goldman, G.H., Walmsley, A.R. and Borges-Walmsley, M.I. 2007. The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis. Molecular Microbiology. 65 (3), pp. 761-779. https://doi.org/10.1111/j.1365-2958.2007.05824.x
Type | Article |
---|---|
Title | The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis |
Authors | Chen, D., Janganan, T.K., Chen, G., Marques, E.R., Kress, M.R., Goldman, G.H., Walmsley, A.R. and Borges-Walmsley, M.I. |
Abstract | Paracoccidioides brasiliensis is a human pathogenic fungus that switches from a saprobic mycelium to a pathogenic yeast. Consistent with the morphological transition being regulated by the cAMP-signalling pathway, there is an increase in cellular cAMP levels both transiently at the onset (< 24 h) and progressively in the later stages (> 120 h) of the transition to the yeast form, and this transition can be modulated by exogenous cAMP. We have cloned the cyr1 gene encoding adenylate cyclase (AC) and established that its transcript levels correlate with cAMP levels. In addition, we have cloned the genes encoding three Gα (Gpa1–3), Gβ (Gpb1) and Gγ (Gpg1) G proteins. Gpa1 and Gpb1 interact with one another and the N-terminus of AC, but neither Gpa2 nor Gpa3 interacted with Gpb1 or AC. The interaction of Gpa1 with Gpb1 was blocked by GTP, but its interaction with AC was independent of bound nucleotide. The transcript levels for gpa1, gpb1 and gpg1 were similar in mycelium, but there was a transient excess of gpb1 during the transition, and an excess of gpa1 in yeast. We have interpreted our findings in terms of a novel signalling mechanism in which the activity of AC is differentially modulated by Gpa1 and Gpb1 to maintain the signal over the 10 days needed for the morphological switch. |
Sustainable Development Goals | 3 Good health and well-being |
Middlesex University Theme | Health & Wellbeing |
Publisher | Wiley |
Journal | Molecular Microbiology |
ISSN | 0950-382X |
Electronic | 1365-2958 |
Publication dates | |
Online | 16 Jul 2007 |
16 Jul 2007 | |
Publication process dates | |
Accepted | 07 Jun 2007 |
Deposited | 12 Nov 2024 |
Output status | Published |
Publisher's version | License File Access Level Open |
Copyright Statement | Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. https://creativecommons.org/licenses/by/2.5/deed.en |
Digital Object Identifier (DOI) | https://doi.org/10.1111/j.1365-2958.2007.05824.x |
https://repository.mdx.ac.uk/item/1w65y5
Download files
Publisher's version
Molecular Microbiology - 2007 - Chen - The cAMP pathway is important for controlling the morphological switch to the.pdf | ||
License: CC BY | ||
File access level: Open |
3
total views2
total downloads0
views this month1
downloads this month