Targeted retroviral transduction of c-kit+ hematopoietic cells using novel ligand display technology

Article

Chandrashekran, A., Gordon, M. and Casimir, C. 2004. Targeted retroviral transduction of c-kit+ hematopoietic cells using novel ligand display technology. Blood. 104 (9), pp. 2697-2703. https://doi.org/10.1182/blood-2003-10-3717
TypeArticle
TitleTargeted retroviral transduction of c-kit+ hematopoietic cells using novel ligand display technology
AuthorsChandrashekran, A., Gordon, M. and Casimir, C.
Abstract

Gene therapy for a wide variety of disorders would be greatly enhanced by the development of vectors that could be targeted for gene delivery to specific populations of cells. We describe here high-efficiency targeted transduction based on a novel targeting strategy that exploits the ability of retroviruses to incorporate host cell proteins into the surface of the viral particle as they bud through the plasma membrane. Ecotropic retroviral particles produced in cells engineered to express the membrane-bound form of stem cell factor (mbSCF) transduce both human cell lines and primary cells with high efficiency in a strictly c-kit (SCF receptor)-dependent fashion. The availability of efficient targeted vectors provides a platform for the development of a new generation of therapies using in vivo gene delivery.

Research GroupMolecular Biology group
PublisherAmerican Society of Hematology
JournalBlood
ISSN0006-4971
Publication dates
Print2004
Publication process dates
Deposited02 Dec 2009
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1182/blood-2003-10-3717
LanguageEnglish
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