Noninflammatory phagocytosis of monosodium urate monohydrate crystals by mouse macrophages: implications for the control of joint inflammation in gout

Article


Yagnik, D., Hillyer, P., Marshall, D., Smythe, C., Krausz, T., Haskard, D. and Landis, R. 2000. Noninflammatory phagocytosis of monosodium urate monohydrate crystals by mouse macrophages: implications for the control of joint inflammation in gout. Arthritis and Rheumatism. 43 (8), pp. 1779-1789. https://doi.org/10.1002/1529-0131(200008)43:8<1779::aid-anr14>3.0.co;2-2
TypeArticle
TitleNoninflammatory phagocytosis of monosodium urate monohydrate crystals by mouse macrophages: implications for the control of joint inflammation in gout
AuthorsYagnik, D., Hillyer, P., Marshall, D., Smythe, C., Krausz, T., Haskard, D. and Landis, R.
Abstract

Objective
We have hypothesized that the process of monocyte to macrophage differentiation may alter the inflammatory response of mononuclear phagocytes to the uptake of monosodium urate monohydrate (MSU) crystals.
Methods
Eight mouse monocyte/macrophage cell lines were arranged in increasing order of differentiation, as judged by expression of the macrophage markers F4/80 and BM 8 and by phagocytic capacity. Secretion of tumor necrosis factor α (TNFα) in response to MSU was measured by enzyme-linked immunosorbent assay.
Results
The panel of monocyte/macrophage cell lines revealed a close linkage between the state of differentiation and the capacity of the cells to ingest MSU crystals. TNFα production, however, was not linked to phagocytic ability. Peak TNFα levels were synthesized by cells at an intermediate state of differentiation (3.2–14.1 ng/ml), whereas mature macrophages, which efficiently phagocytosed crystals, did not secrete TNFα. Mature cell lines produced TNFα when stimulated with zymosan (5.9–6.2 ng/ml), but this was abolished by coincubation with MSU crystals. Suppression of the zymosan response was not due to apoptosis or steric hindrance by MSU crystals. Culture supernatants from mature macrophages did not stimulate endothelial cell activation, in contrast to MSU-treated cells at an earlier stage of differentiation, which stimulated intercellular adhesion molecule 1 expression on sEND endothelioma cells through the release of TNFα (inhibited 80.6% by anti-TNFα).
Conclusion
We demonstrated that phagocytosis and TNFα production are distinct events in the response of mononuclear phagocytes to urate crystals, and these events can be distinguished at the level of macrophage differentiation. The noninflammatory removal of urate crystals by mature macrophages defines a new pathway that may be important in controlling the development of acute gout in patients with hyperuricemia.

PublisherWiley
JournalArthritis and Rheumatism
ISSN0004-3591
Electronic1529-0131
Publication dates
PrintAug 2000
Online26 Mar 2001
Publication process dates
Submitted28 Dec 1999
Accepted05 Apr 2000
Deposited17 Jun 2009
Output statusPublished
Web address (URL)http://onlinelibrary.wiley.com/doi/10.1002/1529-0131(200008)43:8%3C1779::AID-ANR14%3E3.0.CO;2-2/abstract;jsessionid=6D1E1B55AA9F6377387A7D135E616DD9.d03t04
Digital Object Identifier (DOI)https://doi.org/10.1002/1529-0131(200008)43:8<1779::aid-anr14>3.0.co;2-2
PubMed ID10943868
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/81qy0

  • 57
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli
Yagnik, D., Ward, M. and Shah, A. 2021. Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli. Scientific Reports. 11 (1), pp. 1-7. https://doi.org/10.1038/s41598-020-78407-x
Coenzyme Q10 and vitamin D interventions could ameliorate COVID-19 related cellular bioenergetic dysfunction and cytokine storms
Yagnik, D. 2021. Coenzyme Q10 and vitamin D interventions could ameliorate COVID-19 related cellular bioenergetic dysfunction and cytokine storms. Journal of Immunological Sciences. 5 (3), pp. 1-6. https://doi.org/10.29245/2578-3009/2021/3.1220
Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run
Dimitriou, L., Yagnik, D., Wood, A., Sinclair, S., Paice, K., Wilson, L., Hills, F., Gondek, M., Kwan Xue Bin, K., Howatson, G. and Cockburn, E. 2018. Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run. 23rd annual congress of the European College of Sport Science. Dublin, Ireland 04 - 07 Jul 2018
Can quantification of Serum Glycans predict Pre-Eclampsia?
Antia, I., Shah, A., Yagnik, D., Syngelaki, A., Nicolaides, K. and Hills, F. 2018. Can quantification of Serum Glycans predict Pre-Eclampsia? International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e38-e39 https://doi.org/10.1016/j.placenta.2018.06.004
The potential use of microneedles as an alternative method for skin cancer treatment
Loizidou, E., Yagnik, D., Szalecka, M. and Nguyen, D. 2019. The potential use of microneedles as an alternative method for skin cancer treatment. The World Congress on Advanced Treatments and Technologies in Skin Cancer (Skin-Cancer2019). Vienna, Austria 04 - 05 Apr 2019
Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone
Yagnik, D. and Hills, F. 2018. Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone. Molecular Medicine Reports. 18 (3), pp. 3506-3512. https://doi.org/10.3892/mmr.2018.9323
Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion
Yagnik, D., Serafim, V., Choudhury, M. and Shah, A. 2017. Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion. International Conference on Infection Control and Clinical Microbiology. Chicago, USA 25 - 26 Sep 2017 SciTechnol. pp. 73 https://doi.org/10.4172/2329-9541-C1-006
Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression
Yagnik, D., Serafin, V. and Shah, A. 2018. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression. Scientific Reports. 8 (1). https://doi.org/10.1038/s41598-017-18618-x
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, I., Mathew, K., Yagnik, D., Hills, F. and Shah, A. 2018. Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. Analytical and Bioanalytical Chemistry. 410 (1), pp. 131-143. https://doi.org/10.1007/s00216-017-0703-1
Platelet activating acetylhydrolase involved in inflammation resolution in macrophages and neutrophils which have been stimulated with urate crystals
Yagnik, D. 2015. Platelet activating acetylhydrolase involved in inflammation resolution in macrophages and neutrophils which have been stimulated with urate crystals. The 12th World Congress on Inflammation. Boston, USA 08 - 12 Aug 2015 Springer. https://doi.org/10.1007/s00011-015-0839-4
Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring
Antia, I., Yagnik, D., Pantoja Munoz, L., Shah, A. and Hills, F. 2017. Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry. 530, pp. 17-30. https://doi.org/10.1016/j.ab.2017.04.019
Macrophage derived platelet activating factor implicated in the resolution phase of gouty inflammation
Yagnik, D. 2014. Macrophage derived platelet activating factor implicated in the resolution phase of gouty inflammation. International Journal of Inflammation. 2014, pp. 1-6. https://doi.org/10.1155/2014/526496
Monocyte Fc gamma receptor expression in patients undergoing coronary artery bypass grafting
Stefanou, D., Asimakopoulos, G., Yagnik, D., Haskard, D., Anderson, J., Philippidis, P., Landis, R. and Taylor, K. 2004. Monocyte Fc gamma receptor expression in patients undergoing coronary artery bypass grafting. Annals of Thoracic Surgery. 77 (3), pp. 951-955.
Macrophage release of transforming growth factor beta1 during resolution of monododium urate monohydrate crystal-induced inflammation
Yagnik, D., Evans, B., Florey, O., Mason, J., Landis, R. and Haskard, D. 2004. Macrophage release of transforming growth factor beta1 during resolution of monododium urate monohydrate crystal-induced inflammation. Arthritis and Rheumatism. 50 (7), pp. 2273-2280. https://doi.org/10.1002/art.20317
Safe disposal of inflammatory monosodium urate monohydrate crystals by differentiated macrophages
Landis, R., Yagnik, D., Florey, O., Philippidis, P., Emons, V., Mason, J. and Haskard, D. 2002. Safe disposal of inflammatory monosodium urate monohydrate crystals by differentiated macrophages. Arthritis and Rheumatism. 46 (11), pp. 3026-3033. https://doi.org/10.1002/art.10614