Macrophage derived platelet activating factor implicated in the resolution phase of gouty inflammation
Article
Yagnik, D. 2014. Macrophage derived platelet activating factor implicated in the resolution phase of gouty inflammation. International Journal of Inflammation. 2014, pp. 1-6. https://doi.org/10.1155/2014/526496
Type | Article |
---|---|
Title | Macrophage derived platelet activating factor implicated in the resolution phase of gouty inflammation |
Authors | Yagnik, D. |
Abstract | Human blood derived in vitro differentiated monocytes or macrophages are a population of cells which have been investigated over the years to determine the role these cells play in the resolution phase of gout. Macrophages are able to phagocytose monosodium urate monohydrate (MSU) crystals without releasing inflammatory factors. This study analysed macrophage platelet activating factor secretion and its possible role in the pathway of gout resolution. Analysis of sunatants from in vitro differentiated macrophages stimulated with MSU crystals revealed the secretion of platelet activating factor (PAF) mean ± SEM; ng/mL per 106 cells. This secretion was absent in immature monocytes treated similarly. When these monocytes were pretreated with recombinant human PAF-acetylhydrolase (rhuPAF-AH) and MSU crystals resulted in TNFα suppression. Addition of WEB2086, a platelet activating factor (PAF) antagonist, to differentiated macrophages with MSU crystals unmasked TNFα secretion mean ± SEM; ng/mL per 106 cells. This study identifies a role for PAF and the PAF receptor antagonist in the pathway by which macrophages ingest MSU crystals and resolve the concomitant inflammation. |
Research Group | Biomarkers for Cancer group |
Publisher | Hindawi |
Journal | International Journal of Inflammation |
ISSN | 2090-8040 |
Electronic | 2042-0099 |
Publication dates | |
24 Sep 2014 | |
Publication process dates | |
Deposited | 15 Jun 2017 |
Submitted | 16 Jun 2014 |
Accepted | 05 Sep 2014 |
Output status | Published |
Publisher's version | License |
Copyright Statement | Copyright © 2014 Darshna Yagnik. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Digital Object Identifier (DOI) | https://doi.org/10.1155/2014/526496 |
Web of Science identifier | MEDLINE:25328755 |
Language | English |
https://repository.mdx.ac.uk/item/8705w
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