Antifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films

Article

Cossu, A., Wang, M., Chaudhari, A. and Nitin, N. 2015. Antifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films. International Journal of Pharmaceutics. 493 (1-2), pp. 233-242. https://doi.org/10.1016/j.ijpharm.2015.07.065
TypeArticle
TitleAntifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films
AuthorsCossu, A., Wang, M., Chaudhari, A. and Nitin, N.
Abstract

Conventional antifungal treatments against Candida albicans in the oral cavity often result in increased cytotoxicity. The goal of this study was to determine the potential of starch Pickering emulsion as a delivery vehicle for an antifungal natural phenolic compound such as thymol in simulated saliva fluid (SSF) compared to amphotericin B. An oil-in-water (o/w) emulsion was stabilized using starch particles. Physical stability of the emulsion and disruption induced by α-amylase activity in SSF was evaluated. Encapsulated thymol in o/w emulsion was compared to encapsulated amphotericin B for antifungal activity against C. albicans in suspension using emulsions or zone inhibition assay on agar plates using emulsions dispersed in alginate films. Results showed that the emulsions were stable for at least three weeks. Digestion of the emulsion by α-amylase led to coalescence of emulsion droplets. The antifungal activity of thymol and amphotericin B in emulsion formulation was enhanced upon incubation with α-amylase. Results from the zone inhibition assay demonstrated efficacy of the emulsions dispersed in alginate films. Interestingly, addition of α-amylase to the alginate films resulted in a decreased inhibitory effect. Overall, this study showed that starch Pickering emulsions have a potential to deliver hydrophobic antifungal compounds to treat oral candidiasis.

KeywordsCandida albicans; Pickering emulsion ; Alginate film; Controlled-release; Antifungal compound
PublisherElsevier
JournalInternational Journal of Pharmaceutics
ISSN0378-5173
Electronic1873-3476
Publication dates
Online29 Jul 2015
Print01 Sep 2015
Publication process dates
Deposited27 Nov 2019
Accepted26 Jul 2015
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ijpharm.2015.07.065
Scopus EID2-s2.0-84939421052
Web of Science identifierWOS:000360492000027
LanguageEnglish
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