Rapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment

Article

Cossu, A. and Levin, R. 2014. Rapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment. Food Biotechnology. 28 (2), pp. 96-105. https://doi.org/10.1080/08905436.2014.895946
TypeArticle
TitleRapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment
AuthorsCossu, A. and Levin, R.
Abstract

Detection of low numbers of Salmonella in complex food matrices such as ground beef by polymerase chain reaction (PCR) without enrichment is particularly difficult because of the presence of PCR inhibitors and fat. This study used soluble starch for the removal of fat in ground beef followed by the use of activated carbon coated with milk proteins for the removal of PCR inhibitors prior to conventional PCR and RealTime qPCR. This methodology without pre-enrichment allowed detection with conventional PCR of 5 CFU/g and 1 CFU/g with the real-time qPCR in ground beef containing 7%, 15%, and 27% fat. The total assay time was 5 h from the seeding of a 25 g sample of ground beef to agarose gel detection of amplicons of a 284 bp invA gene fragment specific for Salmonella and 4.5 h for real-time qPCR detection.

KeywordsPCR inhibitors; soluble starch ; coated activated carbon; Salmonella enterica serovar Enteritidis; real-time qPCR; ground beef
PublisherTaylor & Francis (Routledge)
JournalFood Biotechnology
ISSN0890-5436
Electronic1532-4249
Publication dates
Online23 Apr 2014
Print03 Apr 2014
Publication process dates
Deposited27 Nov 2019
Accepted23 Apr 2014
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1080/08905436.2014.895946
Scopus EID2-s2.0-84899707731
Web of Science identifierWOS:000335197700002
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/88686

Log in to edit
  • 59
    total views
  • 0
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

Increasing the agricultural sustainability of closed agrivoltaic systems with the integration of vertical farming: A case study on baby-leaf lettuce
Cossu, M., Tiloca, M., Cossu, A., Deligios, P., Pala, T. and Ledda, L. 2023. Increasing the agricultural sustainability of closed agrivoltaic systems with the integration of vertical farming: A case study on baby-leaf lettuce. Applied Energy. 344. https://doi.org/10.1016/j.apenergy.2023.121278
Emerging trends in the photodynamic inactivation (PDI) applied to the food decontamination
Cossu, M., Ledda, L. and Cossu, A. 2021. Emerging trends in the photodynamic inactivation (PDI) applied to the food decontamination. Food Research International. 144. https://doi.org/10.1016/j.foodres.2021.110358
Evaluation of the energy utilization index in sheep milk cooling systems
Cossu, M., Ledda, L., Solinas, S., Cossu, A. and Pazzona, A. 2020. Evaluation of the energy utilization index in sheep milk cooling systems. Energies. 13 (9). https://doi.org/10.3390/en13092127
Agricultural sustainability estimation of the European photovoltaic greenhouses
Cossu, M., Yano, A., Solinas, S., Deligios, P., Tiloca, M., Cossu, A. and Ledda, L. 2020. Agricultural sustainability estimation of the European photovoltaic greenhouses. European Journal of Agronomy. 118, pp. 1-12. https://doi.org/10.1016/j.eja.2020.126074
Enhanced bacterial inactivation in apple juice by synergistic interactions between phenolic acids and mild food processing technologies
de Oliveira, E., Nguyen, C., Stepanian, K., Cossu, A. and Nitin, N. 2019. Enhanced bacterial inactivation in apple juice by synergistic interactions between phenolic acids and mild food processing technologies. Innovative Food Science and Emerging Technologies. 56. https://doi.org/10.1016/j.ifset.2019.102186
Assessment and comparison of the solar radiation distribution inside the main commercial photovoltaic greenhouse types in Europe
Cossu, M., Cossu, A., Deligios, P., Ledda, L., Li, Z., Fatnassi, H., Poncet, C. and Yano, A. 2018. Assessment and comparison of the solar radiation distribution inside the main commercial photovoltaic greenhouse types in Europe. Renewable and Sustainable Energy Reviews. 94, pp. 822-834. https://doi.org/10.1016/j.rser.2018.06.001
Fog, phenolic acids and UV-A light irradiation: a new antimicrobial treatment for decontamination of fresh produce
Cossu, A., Huang, K., Cossu, M., Tikekar, R. and Nitin, N. 2018. Fog, phenolic acids and UV-A light irradiation: a new antimicrobial treatment for decontamination of fresh produce. Food Microbiology. 76, pp. 204-208. https://doi.org/10.1016/j.fm.2018.05.013
Antibiofilm effect of Poly(Vinyl Alcohol-co-Ethylene) Halamine film against Listeria innocua and Escherichia coli O157:H7
Cossu, A., Si, Y., Sun, G. and Nitin, N. 2017. Antibiofilm effect of Poly(Vinyl Alcohol-co-Ethylene) Halamine film against Listeria innocua and Escherichia coli O157:H7. Applied and Environmental Microbiology. 83 (19). https://doi.org/10.1128/aem.00975-17
Enhanced antimicrobial activity based on a synergistic combination of sublethal levels of stresses induced by UV-A light and organic acids
de Oliveira, E., Cossu, A., Tikekar, R. and Nitin, N. 2017. Enhanced antimicrobial activity based on a synergistic combination of sublethal levels of stresses induced by UV-A light and organic acids. Applied and Environmental Microbiology. 83 (11). https://doi.org/10.1128/aem.00383-17
Mechanically robust and transparent N-Halamine grafted PVA-co-PE films with renewable antimicrobial activity
Si, Y., Cossu, A., Nitin, N., Ma, Y., Zhao, C., Chiou, B., Cao, T., Wang, D. and Sun, G. 2017. Mechanically robust and transparent N-Halamine grafted PVA-co-PE films with renewable antimicrobial activity. Macromolecular Bioscience. 17 (3). https://doi.org/10.1002/mabi.201600304
Biomarkers of oxidative damage in bacteria for the assessment of sanitation efficacy in lettuce wash water
Cossu, A., Dou, F., Young, G. and Nitin, N. 2017. Biomarkers of oxidative damage in bacteria for the assessment of sanitation efficacy in lettuce wash water. Applied Microbiology and Biotechnology. 101 (13), pp. 5365-5375. https://doi.org/10.1007/s00253-017-8314-5
An algorithm for the calculation of the light distribution in photovoltaic greenhouses
Cossu, M., Ledda, L., Urracci, G., Sirigu, A., Cossu, A., Murgia, L., Pazzona, A. and Yano, A. 2017. An algorithm for the calculation of the light distribution in photovoltaic greenhouses. Solar Energy. 141, pp. 38-48. https://doi.org/10.1016/j.solener.2016.11.024
Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm
Cossu, A., Ercan, D., Wang, Q., Peer, W., Nitin, N. and Tikekar, R. 2016. Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm. Innovative Food Science and Emerging Technologies. 37 (Part A), pp. 44-52. https://doi.org/10.1016/j.ifset.2016.07.020
Assessment of sanitation efficacy against Escherichia coli O157:H7 by rapid measurement of intracellular oxidative stress, membrane damage or glucose active uptake
Cossu, A., Le, P., Young, G. and Nitin, N. 2017. Assessment of sanitation efficacy against Escherichia coli O157:H7 by rapid measurement of intracellular oxidative stress, membrane damage or glucose active uptake. Food Control. 71, pp. 293-300. https://doi.org/10.1016/j.foodcont.2016.07.009
Synergistic interaction of ultraviolet light and zinc oxide photosensitizer for enhanced microbial inactivation in simulated wash-water
Ercan, D., Cossu, A., Nitin, N. and Tikekar, R. 2016. Synergistic interaction of ultraviolet light and zinc oxide photosensitizer for enhanced microbial inactivation in simulated wash-water. Innovative Food Science and Emerging Technologies. 33, pp. 240-250. https://doi.org/10.1016/j.ifset.2015.11.015
Antimicrobial effect of photosensitized Rose Bengal on bacteria and viruses in model wash water
Cossu, A., Ercan, D., Tikekar, R. and Nitin, N. 2016. Antimicrobial effect of photosensitized Rose Bengal on bacteria and viruses in model wash water. Food and Bioprocess Technology. 9 (3), pp. 441-451. https://doi.org/10.1007/s11947-015-1631-8
Antifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films
Cossu, A., Wang, M., Chaudhari, A. and Nitin, N. 2015. Antifungal activity against Candida albicans of starch Pickering emulsion with thymol or amphotericin B in suspension and calcium alginate films. International Journal of Pharmaceutics. 493 (1-2), pp. 233-242. https://doi.org/10.1016/j.ijpharm.2015.07.065
Fat removal with hydrolyzed corn starch for real-time qPCR detection of Salmonella enterica in ground beef in 4.5 hours without enrichment
Cossu, A., Witkowsky, R. and Levin, R. 2014. Fat removal with hydrolyzed corn starch for real-time qPCR detection of Salmonella enterica in ground beef in 4.5 hours without enrichment. Food Control. 46, pp. 475-479. https://doi.org/10.1016/j.foodcont.2014.06.009
Expression profiling of Mycobacterium tuberculosis H37Rv and Mycobacterium smegmatis in acid-nitrosative multi-stress displays defined regulatory networks
Cossu, A., Sechi, L., Bandino, E., Zanetti, S. and Rosu, V. 2013. Expression profiling of Mycobacterium tuberculosis H37Rv and Mycobacterium smegmatis in acid-nitrosative multi-stress displays defined regulatory networks. Microbial Pathogenesis. 65, pp. 89-96. https://doi.org/10.1016/j.micpath.2013.10.004
Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis's MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine
Cossu, A., Ferrannini, E., Fallahi, P., Antonelli, A. and Sechi, L. 2013. Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis's MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine. Cytokine. 61 (2), pp. 337-339. https://doi.org/10.1016/j.cyto.2012.11.008
Unraveling the transcriptional regulatory networks associated to mycobacterial cell wall defective form induction by glycine and lysozyme treatment
Rosu, V., Bandino, E. and Cossu, A. 2013. Unraveling the transcriptional regulatory networks associated to mycobacterial cell wall defective form induction by glycine and lysozyme treatment. Microbiological Research. 168 (3), pp. 153-164. https://doi.org/10.1016/j.micres.2012.10.002
Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour
Cossu, A., Sechi, L., Zanetti, S. and Rosu, V. 2012. Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour. BMC Microbiology. 12. https://doi.org/10.1186/1471-2180-12-87
MAP3738c and MptD are specific tags of Mycobacterium avium subsp. paratuberculosis infection in type I diabetes mellitus
Cossu, A., Rosu, V., Paccagnini, D., Cossu, D., Pacifico, A. and Sechi, L. 2011. MAP3738c and MptD are specific tags of Mycobacterium avium subsp. paratuberculosis infection in type I diabetes mellitus. Clinical Immunology. 141 (1), pp. 49-57. https://doi.org/10.1016/j.clim.2011.05.002
Detection of Mycobacterium avium subsp. paratuberculosis (MAP)-specific IS900 DNA and antibodies against MAP peptides and lysate in the blood of Crohn's disease patients
Di Sabatino, A., Paccagnini, D., Vidali, F., Rosu, V., Biancheri, P., Cossu, A., Zanetti, S., Corazza, G. and Sechi, L. 2011. Detection of Mycobacterium avium subsp. paratuberculosis (MAP)-specific IS900 DNA and antibodies against MAP peptides and lysate in the blood of Crohn's disease patients. Inflammatory Bowel Diseases. 17 (5), pp. 1254-1255. https://doi.org/10.1002/ibd.21461