Rapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment

Article

Cossu, A. and Levin, R. 2014. Rapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment. Food Biotechnology. 28 (2), pp. 96-105. https://doi.org/10.1080/08905436.2014.895946
TypeArticle
TitleRapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella enterica from ground beef without enrichment
AuthorsCossu, A. and Levin, R.
Abstract

Detection of low numbers of Salmonella in complex food matrices such as ground beef by polymerase chain reaction (PCR) without enrichment is particularly difficult because of the presence of PCR inhibitors and fat. This study used soluble starch for the removal of fat in ground beef followed by the use of activated carbon coated with milk proteins for the removal of PCR inhibitors prior to conventional PCR and RealTime qPCR. This methodology without pre-enrichment allowed detection with conventional PCR of 5 CFU/g and 1 CFU/g with the real-time qPCR in ground beef containing 7%, 15%, and 27% fat. The total assay time was 5 h from the seeding of a 25 g sample of ground beef to agarose gel detection of amplicons of a 284 bp invA gene fragment specific for Salmonella and 4.5 h for real-time qPCR detection.

KeywordsPCR inhibitors; soluble starch ; coated activated carbon; Salmonella enterica serovar Enteritidis; real-time qPCR; ground beef
PublisherTaylor & Francis (Routledge)
JournalFood Biotechnology
ISSN0890-5436
Electronic1532-4249
Publication dates
Online23 Apr 2014
Print03 Apr 2014
Publication process dates
Deposited27 Nov 2019
Accepted23 Apr 2014
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1080/08905436.2014.895946
Scopus EID2-s2.0-84899707731
Web of Science identifierWOS:000335197700002
LanguageEnglish
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