International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests

Article

Adcock, D.M., Moore, G., Kershaw, G.W., Montalvao, S. and Gosselin, R. 2024. International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests. International Journal of Laboratory Hematology. 46 (5), pp. 777-788. https://doi.org/10.1111/ijlh.14344

Publication dates
Type	Article
Title	International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests
Authors	Adcock, D.M., Moore, G., Kershaw, G.W., Montalvao, S. and Gosselin, R.
Abstract	This guidance document has been prepared on behalf of the International Council for Standardization in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for the performance and interpretation of activated partial thromboplastin time (APTT) and prothrombin time (PT) plasma mixing tests in clinical laboratories in all regions of the world. The following areas are included in this document: preanalytical, analytical, postanalytical, and quality assurance considerations as they relate to the proper performance and interpretation of plasma mixing tests. The recommendations are based on good laboratory practice, published data in peer-reviewed literature, and expert opinion.
Sustainable Development Goals	3 Good health and well-being
Middlesex University Theme	Health & Wellbeing
Publisher	Wiley
Journal	International Journal of Laboratory Hematology
ISSN	1751-5521
Electronic	1751-553X
Online	15 Jul 2024
Print	Oct 2024
Publication process dates
Submitted	20 Mar 2024
Accepted	27 Jun 2024
Deposited	10 Nov 2025
Output status	Published
Digital Object Identifier (DOI)	https://doi.org/10.1111/ijlh.14344

Permalink -

https://repository.mdx.ac.uk/item/2yqy71

Log in to edit

58
total views
0
total downloads
0
views this month
0
downloads this month

Export as

Related outputs

Diagnosing thrombophilia: the case for genetic or functional testing?

Favaloro, E.J., Moore, G.W. and Pasalic, L. 2025. Diagnosing thrombophilia: the case for genetic or functional testing? Expert Review of Molecular Diagnostics. 25 (12), pp. 841-846. https://doi.org/10.1080/14737159.2025.2588621

Survey on current practice in thrombophilia testing: from phenotype to genotype. Communication from the SSC of the ISTH

Van Laer, C., Moore, G.W., Majumder, R., Corral, J., Freson, K., Ignjatovic, V. and Orlando, C. 2025. Survey on current practice in thrombophilia testing: from phenotype to genotype. Communication from the SSC of the ISTH. Journal of Thrombosis and Haemostasis. https://doi.org/10.1016/j.jtha.2025.10.032

International Council for Standardization in Haematology field study evaluating optimal interpretation methods for activated partial thromboplastin time and prothrombin time mixing studies

Gosselin, R.C., Moore, G.W., Kershaw, G.W., Montalvão, S. and Adcock, D.M. 2023. International Council for Standardization in Haematology field study evaluating optimal interpretation methods for activated partial thromboplastin time and prothrombin time mixing studies. Archives of Pathology & Laboratory Medicine. 148 (8), pp. 880-889. https://doi.org/10.5858/arpa.2023-0030-oa

Analytical dilemmas in lupus anticoagulant detection

Moore, G. 2023. Analytical dilemmas in lupus anticoagulant detection. Exploration of Immunology. 3, pp. 300-324. https://doi.org/10.37349/ei.2023.00104

ADAMTS13 activity measurement by ELISA and fluorescence resonance energy transfer assay

Moore, G., Llusa, M., Griffiths, M. and Binder, N. 2023. ADAMTS13 activity measurement by ELISA and fluorescence resonance energy transfer assay. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.

ADAMTS13 activity: Screening test protocol

Moore, G., Griffiths, M. and Binder, N. 2023. ADAMTS13 activity: Screening test protocol. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.

Lupus anticoagulant testing: Dilute prothrombin time (dPT)

Moore, G. 2023. Lupus anticoagulant testing: Dilute prothrombin time (dPT). in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.

Lupus anticoagulant testing: Taipan snake venom time with ecarin time as confirmatory test

Moore, G. 2023. Lupus anticoagulant testing: Taipan snake venom time with ecarin time as confirmatory test. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.

ADAMTS13 antibody and inhibitor assays

Moore, G., Vetr, H. and Binder, N. 2023. ADAMTS13 antibody and inhibitor assays. in: Favaloro, E. and Gosselin, R. (ed.) Hemostasis and Thrombosis: Methods and Protocols New York, NY Humana.

Activated partial thromboplastin time and prothrombin time mixing studies: current state of the art

Adcock, D., Moore, G., Montalvão, S., Kershaw, G. and Gosselin, R. 2022. Activated partial thromboplastin time and prothrombin time mixing studies: current state of the art. Seminars in Thrombosis and Hemostasis. 49 (6), pp. 571-579. https://doi.org/10.1055/s-0042-1756196

Added value of antiphosphatidylserine/prothrombin antibodies in the workup of thrombotic antiphospholipid syndrome: Communication from the ISTH SSC Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibodies

Vandevelde, A., Chayoua, W., de Laat, B., Moore, G., Musiał, J., Zuily, S., Wahl, D. and Devreese, K. 2022. Added value of antiphosphatidylserine/prothrombin antibodies in the workup of thrombotic antiphospholipid syndrome: Communication from the ISTH SSC Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibodies. Journal of Thrombosis and Haemostasis. 20 (9), pp. 2136-2150. https://doi.org/10.1111/jth.15785

Effects of regional citrate anticoagulation on thrombin generation, fibrinolysis and platelet function in critically ill patients receiving continuous renal replacement therapy for acute kidney injury: a prospective study

Fisher, R., Moore, G., Mitchell, M., Dai, L., Crichton, S., Lumlertgul, N. and Ostermann, M. 2022. Effects of regional citrate anticoagulation on thrombin generation, fibrinolysis and platelet function in critically ill patients receiving continuous renal replacement therapy for acute kidney injury: a prospective study. Annals of Intensive Care. 12 (1), pp. 1-11. https://doi.org/10.1186/s13613-022-01004-w

Direct oral anticoagulants‐Remove versus Taipan snake venom time for detection of a lupus anticoagulant in patients taking oral direct factor Xa inhibitors

White, D., Moore, G., Besser, M., MacDonald, S. and Thomas, W. 2022. Direct oral anticoagulants‐Remove versus Taipan snake venom time for detection of a lupus anticoagulant in patients taking oral direct factor Xa inhibitors. Research and Practice in Thrombosis and Haemostasis. 6 (1), pp. 1-8. https://doi.org/10.1002/rth2.12648

Semiquantitative interpretation of anticardiolipin and antiβ2glycoprotein I antibodies measured with various analytical platforms: communication from the ISTH SSC subcommittee on Lupus Anticoagulant/Antiphospholipid antibodies

Vandevelde, A., Chayoua, W., de Laat, B., Gris, J., Moore, G., Musiał, J., Zuily, S., Wahl, D. and Devreese, K. 2022. Semiquantitative interpretation of anticardiolipin and antiβ2glycoprotein I antibodies measured with various analytical platforms: communication from the ISTH SSC subcommittee on Lupus Anticoagulant/Antiphospholipid antibodies. Journal of Thrombosis and Haemostasis. 20 (2), pp. 508-524. https://doi.org/10.1111/jth.15585

Snake venoms in diagnostic hemostasis and thrombosis

Moore, G. 2022. Snake venoms in diagnostic hemostasis and thrombosis. Seminars in Thrombosis and Hemostasis. 48 (02), pp. 145-160. https://doi.org/10.1055/s-0041-1732465

International multicenter, multiplatform study to validate Taipan snake venom time as a lupus anticoagulant screening test with ecarin time as the confirmatory test: communication from the ISTH SSC subcommittee on Lupus Anticoagulant/Antiphospholipid antibodies

Moore, G., Jones, P., Platton, S., Hussain, N., White, D., Thomas, W., Rigano, J., Pouplard, C., Gray, E. and Devreese, K. 2021. International multicenter, multiplatform study to validate Taipan snake venom time as a lupus anticoagulant screening test with ecarin time as the confirmatory test: communication from the ISTH SSC subcommittee on Lupus Anticoagulant/Antiphospholipid antibodies. Journal of Thrombosis and Haemostasis. 19 (12), pp. 3177-3192. https://doi.org/10.1111/jth.15438

Applying index of circulating anticoagulant to mixing tests with lupus anticoagulant screen and confirm reagents can distinguish with high specificity between lupus anticoagulants and direct factor Xa inhibitors

Kumano, O., Amiral, J., Dunois, C., Peyrafitte, M. and Moore, G. 2021. Applying index of circulating anticoagulant to mixing tests with lupus anticoagulant screen and confirm reagents can distinguish with high specificity between lupus anticoagulants and direct factor Xa inhibitors. International Journal of Laboratory Hematology. 43 (4), pp. 771-778. https://doi.org/10.1111/ijlh.13571

Clinical validation of an automated fluorogenic factor XIII activity assay based on isopeptidase activity

Leitner, M., Büchold, C., Pasternack, R., Binder, N. and Moore, G. 2021. Clinical validation of an automated fluorogenic factor XIII activity assay based on isopeptidase activity. International Journal of Molecular Sciences. 22 (3). https://doi.org/10.3390/ijms22031002