Response of the microbial community to salt stress and its stratified effect in constructed wetlands

Article

Wang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G. and Cheng, R. 2021. Response of the microbial community to salt stress and its stratified effect in constructed wetlands. Environmental Science and Pollution Research. 28 (14), pp. 18089-18101. https://doi.org/10.1007/s11356-020-11937-z
TypeArticle
TitleResponse of the microbial community to salt stress and its stratified effect in constructed wetlands
AuthorsWang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G. and Cheng, R.
Abstract

Nitrogen removal in constructed wetlands (CWs) may be inhibited by salinity. The clarification of the response of microbial community to salt stress is a premise for developing strategies to improve nitrogen removal efficiency in CWs under saline conditions. Results showed that the ammonia nitrogen (NH -N), nitrate nitrogen (NO -N), and total nitrogen (TN) removal percentages significantly (p < 0.05) decreased in CWs with increasing salinity. The structure and abundance of the microbial community varied with different salinity levels and sampling depths in CWs. Compared with a non-saline condition, the abundances of some bacteria with a denitrification function (e.g., Arthrobacter) significantly (p < 0.05) decreased in CWs under saline conditions (i.e., EC of 15 and 30 mS/cm). Aerobic bacteria (e.g., Sphingomonas) exhibited more abundance in soil and upper gravel samples in CWs than those in bottom gravel samples, while the abundance of some denitrifying bacteria (e.g., Thauera and Azoarcus) was significantly (p < 0.05) higher in bottom gravel samples compared with soil and upper gravel samples, respectively. This study provides both microbiological evidence for explaining the impact of salt stress on nitrogen removal in CWs and scientific reference for developing enhanced strategies to improve the nitrogen removal capacity of CWs.

KeywordsConstructed wetlands, High-throughput sequencing technology, Microbial community, Nitrogen removal, Saline wastewater, Salt stress
PublisherSpringer
JournalEnvironmental Science and Pollution Research
ISSN0944-1344
Electronic1614-7499
Publication dates
Online06 Jan 2021
Print30 Apr 2021
Publication process dates
Deposited20 Jan 2021
Submitted25 Sep 2020
Accepted01 Dec 2020
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/s11356-020-11937-z
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/893z1

Log in to edit
  • 41
    total views
  • 0
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

Nitrogen migration and transformation in a saline-alkali paddy ecosystem with application of different nitrogen fertilizers
Wang, X., Wang, M., Chen, L., Shutes, B., Yan, B., Zhang, F., Lyu, J. and Zhu, H. 2023. Nitrogen migration and transformation in a saline-alkali paddy ecosystem with application of different nitrogen fertilizers. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-023-25984-9
Layered double hydroxides, an effective nanomaterial to remove phosphorus from wastewater: Performance, mechanism, factors and reusability
Feng, W., Cui, H., Zhu, H., Shutes, B., Yan, B. and Hou, S. 2023. Layered double hydroxides, an effective nanomaterial to remove phosphorus from wastewater: Performance, mechanism, factors and reusability. Science of the Total Environment. 884. https://doi.org/10.1016/j.scitotenv.2023.163757
Effect of butachlor on microcystis aeruginosa: cellular and molecular mechanisms of toxicity
Yu, J., Zhu, H., Wang, H., Shutes, B. and Niu, T. 2023. Effect of butachlor on microcystis aeruginosa: cellular and molecular mechanisms of toxicity. Journal of Hazardous Materials. 449, p. 131042. https://doi.org/10.1016/j.jhazmat.2023.131042
Factors influencing gaseous emissions in constructed wetlands: a meta-analysis and systematic review
Hu, S., Zhu, H., Bañuelos, G., Shutes, B., Wang, X., Hou, S. and Yan, B. 2023. Factors influencing gaseous emissions in constructed wetlands: a meta-analysis and systematic review. International Journal of Environmental Research and Public Health. 20 (5). https://doi.org/10.3390/ijerph20053876
Integration of MFC reduces CH4, N2O and NH3 emissions in batch-fed wetland systems
Zhu, H., Niu, T., Shutes, B., Wang, X., He, C. and Hou, S. 2022. Integration of MFC reduces CH4, N2O and NH3 emissions in batch-fed wetland systems. Water Research. 226. https://doi.org/10.1016/j.watres.2022.119226
Salt-alkalization may potentially promote Microcystis aeruginosa blooms and the production of microcystin-LR
Yu, J., Zhu, H., Shutes, B. and Wang, X. 2022. Salt-alkalization may potentially promote Microcystis aeruginosa blooms and the production of microcystin-LR. Environmental Pollution. 301, pp. 1-9. https://doi.org/10.1016/j.envpol.2022.118971
Removal of microcystin (MC-LR) in constructed wetlands integrated with microbial fuel cells: Efficiency, bioelectricity generation and microbial response
Cheng, R., Zhu, H., Wang, J., Hou, S., Shutes, B. and Yan, B. 2022. Removal of microcystin (MC-LR) in constructed wetlands integrated with microbial fuel cells: Efficiency, bioelectricity generation and microbial response. Journal of Environmental Management. 309, pp. 1-9. https://doi.org/10.1016/j.jenvman.2022.114669
Biochar-amended constructed wetlands for eutrophication control and microcystin (MC-LR) removal
Cheng, R., Hou, S., Wang, J., Zhu, H., Shutes, B. and Yan, B. 2022. Biochar-amended constructed wetlands for eutrophication control and microcystin (MC-LR) removal. Chemosphere. 295, pp. 1-11. https://doi.org/10.1016/j.chemosphere.2022.133830
High removal efficiencies of antibiotics and low accumulation of antibiotic resistant genes obtained in microbial fuel cell-constructed wetlands intensified by sponge iron
Wen, H., Zhu, H., Yan, B., Bañuelos, G., Shutes, B., Wang, X., Cao, S., Cheng, R. and Tian, L. 2022. High removal efficiencies of antibiotics and low accumulation of antibiotic resistant genes obtained in microbial fuel cell-constructed wetlands intensified by sponge iron. Science of the Total Environment. 806 (Part 1). https://doi.org/10.1016/j.scitotenv.2021.150220
Improving denitrification efficiency in constructed wetlands integrated with immobilized bacteria under high saline conditions
Wang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G., Wen, H. and Cheng, R. 2021. Improving denitrification efficiency in constructed wetlands integrated with immobilized bacteria under high saline conditions. Environmental Pollution. 287. https://doi.org/10.1016/j.envpol.2021.117592
Removal of chlorpyrifos and its hydrolytic metabolite in microcosm-scale constructed wetlands under soda saline-alkaline condition: mass balance and intensification strategies
Zhu, H., Yu, X., Xu, Y., Yan, B., Bañuelos, G., Shutes, B. and Wen, Z. 2021. Removal of chlorpyrifos and its hydrolytic metabolite in microcosm-scale constructed wetlands under soda saline-alkaline condition: mass balance and intensification strategies. Science of the Total Environment. 777, pp. 1-11. https://doi.org/10.1016/j.scitotenv.2021.145956
Treatment of microcystin (MC-LR) and nutrients in eutrophic water by constructed wetlands: Performance and microbial community
Cheng, R., Zhu, H., Shutes, B. and Yan, B. 2021. Treatment of microcystin (MC-LR) and nutrients in eutrophic water by constructed wetlands: Performance and microbial community. Chemosphere. 263. https://doi.org/10.1016/j.chemosphere.2020.128139
Removal of sulfamethoxazole and tetracycline in constructed wetlands integrated with microbial fuel cells influenced by influent and operational conditions
Wen, H., Zhu, H., Xu, Y., Yan, B., Shutes, B., Bañuelos, G. and Wang, X. 2021. Removal of sulfamethoxazole and tetracycline in constructed wetlands integrated with microbial fuel cells influenced by influent and operational conditions. Environmental Pollution. 272. https://doi.org/10.1016/j.envpol.2020.115988
Constructed wetlands integrated with microbial fuel cells for COD and nitrogen removal affected by plant and circuit operation mode
Wen, H., Zhu, H., Yan, B., Shutes, B., Yu, X., Cheng, R., Chen, X. and Wang, X. 2021. Constructed wetlands integrated with microbial fuel cells for COD and nitrogen removal affected by plant and circuit operation mode. Environmental Science and Pollution Research. 28 (3), pp. 3008-3018. https://doi.org/10.1007/s11356-020-10632-3
Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water
Cheng, R., Wang, X., Zhu, H., Yan, B., Shutes, B., Xu, Y., Fu, B. and Wen, H. 2020. Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water. Scientific Reports. 10 (1). https://doi.org/10.1038/s41598-020-66989-5
Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water
Cheng, R., Wang, X., Zhu, H., Yan, B., Shutes, B., Xu, Y., Fu, B. and Wen, H. 2020. Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water. Scientific Reports. 10 (1). https://doi.org/10.1038/s41598-020-66989-5
Bioaugmented constructed wetlands for denitrification of saline wastewater: a boost for both microorganisms and plants
Wang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G. and Wen, H. 2020. Bioaugmented constructed wetlands for denitrification of saline wastewater: a boost for both microorganisms and plants. Environment International. 138. https://doi.org/10.1016/j.envint.2020.105628
Treatment of typical antibiotics in constructed wetlands integrated with microbial fuel cells: roles of plant and circuit operation mode
Wen, H., Zhu, H., Yan, B., Xu, Y. and Shutes, B. 2020. Treatment of typical antibiotics in constructed wetlands integrated with microbial fuel cells: roles of plant and circuit operation mode. Chemosphere. 250. https://doi.org/10.1016/j.chemosphere.2020.126252
Saline and Alkaline tolerance of wetland plants — what are the most representative evaluation indicators?
Cheng, R., Zhu, H., Cheng, X., Shutes, B. and Yan, B. 2020. Saline and Alkaline tolerance of wetland plants — what are the most representative evaluation indicators? Sustainability. 12 (5). https://doi.org/10.3390/su12051913
Greenhouse gas emissions and wastewater treatment performance by three plant species in subsurface flow constructed wetland mesocosms
Chen, X., Zhu, H., Yan, B., Shutes, B., Xing, D., Banuelos, G., Cheng, R. and Wang, X. 2020. Greenhouse gas emissions and wastewater treatment performance by three plant species in subsurface flow constructed wetland mesocosms. Chemosphere. 239. https://doi.org/10.1016/j.chemosphere.2019.124795
Identification and denitrification characteristics of a salt-tolerant denitrifying bacterium Pannonibacter phragmitetus F1
Wang, X., Zhu, H., Shutes, B., Fu, B., Yan, B., Yu, X., Wen, H. and Chen, X. 2019. Identification and denitrification characteristics of a salt-tolerant denitrifying bacterium Pannonibacter phragmitetus F1. AMB Express. 9 (1), pp. 1-11. https://doi.org/10.1186/s13568-019-0918-y
Influence of salt stress on propagation, growth and nutrient uptake of typical aquatic plant species
Chen, X., Cheng, X., Zhu, H., Bañuelos, G., Shutes, B. and Wu, H. 2019. Influence of salt stress on propagation, growth and nutrient uptake of typical aquatic plant species. Nordic Journal of Botany. 37 (12). https://doi.org/10.1111/njb.02411
Removal of chlorpyrifos and its hydrolytic metabolite 3,5,6-trichloro-2-pyridinol in constructed wetland mesocosms under soda saline-alkaline conditions: Effectiveness and influencing factors
Yu, X., Zhu, H., Yan, B., Xu, Y., Bañuelos, G., Shutes, B., Wen, H. and Cheng, R. 2019. Removal of chlorpyrifos and its hydrolytic metabolite 3,5,6-trichloro-2-pyridinol in constructed wetland mesocosms under soda saline-alkaline conditions: Effectiveness and influencing factors. Journal of Hazardous Materials. 373, pp. 67-74. https://doi.org/10.1016/j.jhazmat.2019.03.052
Sustainable ecological restoration of brownfield sites through engineering or managed natural attenuation? a case study from Northwest England
Hartley, W., Dickinson, N., Riby, P. and Shutes, B. 2012. Sustainable ecological restoration of brownfield sites through engineering or managed natural attenuation? a case study from Northwest England. Ecological Engineering. 40, pp. 70-79. https://doi.org/10.1016/j.ecoleng.2011.12.020
Planting woody crops on dredged contaminated sediment provides both positive and negative effects in terms of remediation
Hartley, W., Riby, P., Dickinson, N., Shutes, B., Sparke, S. and Scholz, M. 2011. Planting woody crops on dredged contaminated sediment provides both positive and negative effects in terms of remediation. Environmental Pollution. 159 (12), pp. 3416-3424. https://doi.org/10.1016/j.envpol.2011.08.030
Constructed wetlands for flood prevention and water reuse
Shutes, B., Revitt, D. and Lundy, L. 2010. Constructed wetlands for flood prevention and water reuse. 12th International Conference on Wetland Systems for Water Pollution Control. Venice 04 - 08 Oct 2010
Sustainable stormwater management in the city of the future
Shutes, B., Revitt, D., Ellis, J. and Lundy, L. 2010. Sustainable stormwater management in the city of the future. International forum on construction of water system and waterfront living environment in urban areas.. Kaohsiung, Taiwan
Constructed wetland control of BOD levels in airport runoff
Adeola, S., Revitt, D., Shutes, B., Garelick, H., Jones, H. and Jones, C. 2009. Constructed wetland control of BOD levels in airport runoff. International Journal of Phytoremediation. 11 (1), pp. 1-10. https://doi.org/10.1080/15226510802363220
Effects of temperature on metal tolerance and the accumulation of Zn and Pb by metal-tolerant fungi isolated from urban runoff treatment wetlands
Purchase, D., Lundy, L., Revitt, D. and Shutes, B. 2009. Effects of temperature on metal tolerance and the accumulation of Zn and Pb by metal-tolerant fungi isolated from urban runoff treatment wetlands. Journal of Applied Microbiology. 106 (4), pp. 1163-1174. https://doi.org/10.1111/j.1365-2672.2008.04082.x
The role of constructed wetlands in cities of the future. A strategy for creating ‘utopia’
Shutes, B., Revitt, D., Ellis, J. and Lundy, L. 2008. The role of constructed wetlands in cities of the future. A strategy for creating ‘utopia’. 11th International Conference on Wetland Systems for Water Pollution Control. Indore, India 01 - 07 Nov 2008
Environmental factors influencing the prevalence of respiratory diseases and allergies among schoolchildren in Chiang Mai, Thailand
Sriyaraj, K., Priest, N. and Shutes, B. 2008. Environmental factors influencing the prevalence of respiratory diseases and allergies among schoolchildren in Chiang Mai, Thailand. International Journal of Environmental Health Research. 18 (2), pp. 129-148. https://doi.org/10.1080/09603120701694109
Nutrient removal in a pilot and full scale constructed wetland, Putrajaya City, Malaysia
Shutes, B., Ho, S., Sim, C. and Yusoff, M. 2008. Nutrient removal in a pilot and full scale constructed wetland, Putrajaya City, Malaysia. Journal of Environmental Management. 88 (2), pp. 307-317. https://doi.org/10.1016/j.jenvman.2007.03.011
The collection and comparison of stakeholders' opinions on urban stormwater control measures: consulting experience within the UK
Shutes, B., Oldham, J. and Lundy, L. 2006. The collection and comparison of stakeholders' opinions on urban stormwater control measures: consulting experience within the UK. 17th Scientific sessions of the environment: the city, the citizen & the environment. Hotel du Department, Créteil 23 - 24 May 2006
Landfill leachate treatment by an experimental subsurface flow constructed wetland in tropical climate countries
Shutes, B., Ujang, Z., Soedjono, E. and Salim, M. 2005. Landfill leachate treatment by an experimental subsurface flow constructed wetland in tropical climate countries. Water Science & Technology. 52 (12), pp. 243-250.
The performance of vegetative treatment systems for highway runoff during dry and wet weather conditions
Revitt, D., Forshaw, M., Jones, H. and Shutes, B. 2004. The performance of vegetative treatment systems for highway runoff during dry and wet weather conditions. Science of the Total Environment. 334-335. https://doi.org/10.1016/j.scitotenv.2004.04.046
Guidance manual for constructed wetlands
Ellis, J., Shutes, B. and Revitt, D. 2003. Guidance manual for constructed wetlands. Environment Agency.
Constructed wetlands and links with sustainable drainage systems
Ellis, J., Shutes, B. and Revitt, D. 2003. Constructed wetlands and links with sustainable drainage systems. Environment Agency.
Overcoming the dark side: issues and protocols for urban stormwater wetland operation and maintenance
Ellis, J., Revitt, D. and Shutes, B. 2003. Overcoming the dark side: issues and protocols for urban stormwater wetland operation and maintenance. 2nd National UK Conference on Sustainable Drainage. Coventry, UK 23 - 24 Jun 2003
Practical experiences of the use of vegetated ponds for the treatment of highway runoff
Revitt, D., Shutes, B., Jones, H., Forshaw, M. and Winter, B. 2003. Practical experiences of the use of vegetated ponds for the treatment of highway runoff. Pratt, C., Davies, J., Newman, A. and Perry, J. (ed.) 2nd National UK Conference on Sustainable Drainage. Coventry, UK 23 - 24 Jun 2003 School of Science and the Environment, Coventry University. pp. 19-29
Hydrocarbon removal in an experimental gravel bed constructed wetland
Shutes, B., Katima, J., Omari, K., Revitt, D. and Garelick, H. 2003. Hydrocarbon removal in an experimental gravel bed constructed wetland. Water Science & Technology. 48 (5), pp. 275-282. https://doi.org/10.2166/wst.2003.0333
Treatment of agricultural wastewater in two experimental combined constructed wetland systems in a tropical climate
Kantawanichkul, S., Somprasert, S., Aekasin, U. and Shutes, B. 2003. Treatment of agricultural wastewater in two experimental combined constructed wetland systems in a tropical climate. Water Science & Technology. 48 (5), pp. 199-205. https://doi.org/10.2166/wst.2003.0319
The effect of early coastal development on the fringing coral reefs of Langkawi: a study in small scale changes
Shutes, B., Abdullah, A., Yasin, Z., Ismail, W. and Fitzsimons, M. 2002. The effect of early coastal development on the fringing coral reefs of Langkawi: a study in small scale changes. Malaysian Journal of Remote Sensing and GIS. 3, pp. 1-10.
Nutrient removal in a combined system: vertical vegetated bed over horizontal flow sand bed
Shutes, B., Kantawanichkul, S. and Neamkam, P. 2001. Nutrient removal in a combined system: vertical vegetated bed over horizontal flow sand bed. Water Science & Technology. 44 (11), pp. 137-142.
An experimental constructed wetland system for the treatment of highway runoff in the UK
Shutes, B., Revitt, D., Lundy, L., Forshaw, M. and Winter, B. 2001. An experimental constructed wetland system for the treatment of highway runoff in the UK. Water Science & Technology. 44 (11), pp. 571-578.
Artificial wetlands and water quality improvement
Shutes, B. 2001. Artificial wetlands and water quality improvement. Environment International. 26 (5-6), pp. 441-447. https://doi.org/10.1016/S0160-4120(01)00025-3
An assessment of the impact of motorway runoff on a pond, wetland and stream
Shutes, B. and Sriyaraj, K. 2001. An assessment of the impact of motorway runoff on a pond, wetland and stream. Environment International. 26 (5-6), pp. 433-439. https://doi.org/10.1016/S0160-4120(01)00024-1
The microbiology associated with glycol removal in constructed wetlands
Chong, S., Garelick, H., Revitt, D., Shutes, B., Worrall, P. and Brewer, D. 1999. The microbiology associated with glycol removal in constructed wetlands. Water Science & Technology. 40 (3), pp. 99-107. https://doi.org/10.2166/wst.1999.0145
The biological impacts of urban runoff waters
Shutes, B. 1995. The biological impacts of urban runoff waters. PhD thesis Middlesex University School of Health & Education