Wang, X.; Zhu, H.; Yan, B.; Shutes, B.; Bañuelos, G.; Wen, H.; Cheng, R.

Improving denitrification efficiency in constructed wetlands integrated with immobilized bacteria under high saline conditions

Article

Wang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G., Wen, H. and Cheng, R. 2021. Improving denitrification efficiency in constructed wetlands integrated with immobilized bacteria under high saline conditions. Environmental Pollution. 287. https://doi.org/10.1016/j.envpol.2021.117592

Publication dates
Type	Article
Title	Improving denitrification efficiency in constructed wetlands integrated with immobilized bacteria under high saline conditions
Authors	Wang, X., Zhu, H., Yan, B., Shutes, B., Bañuelos, G., Wen, H. and Cheng, R.
Abstract	Constructed wetlands (CWs) inoculated with exogenous microbes have great potential for removing pollutants in adverse environments. The rapid loss of functional bacteria and the high cost of repeated additions of inoculum, however, limit the practical application of this technology. In this study, C-F2 immobilized bacteria (i.e., immobilized salt-tolerant bacterium Alishewanella sp. F2 incorporated with a carbon source) were developed and utilized in CWs for solving the above problems. A 60-day experiment demonstrated that bioaugmented CWs (Bio-CWs) with the addition of C-F2 immobilized bacteria into the bottom gravel layer of CW microcosms (B-CF2 treatment) exhibited high nitrogen removal efficiency under a saline condition (electrical conductivity of 15 mS/cm). We measured mean nitrate nitrogen (NO -N) and total nitrogen (TN) removal percentages of 97.8% and 88.1%, respectively, which were significantly (p < 0.05) higher than those in Bio-CWs with microbial inoculum (MI-F2 treatment, 63.5% and 78.2%) and unbioaugmented CWs (CK, 48.7% and 67.2%). The TN content of the entire plant was significantly (p < 0.05) increased in B-CF2 (636.06 mg/microcosm) compared with CK (372.06 mg/microcosm). The relative abundances of the genera Alishewanella (i.e., the exogenous bacterium, 5.5%), Clostridium-XlVa (8.8%) and Bacteroides (21.1%) in B-CF2 were significantly (p < 0.05) higher than in MI-F2 and CK, which improved the denitrification capacity of CWs. Overall, a high denitrification efficiency and durability were achieved in the newly developed Bio-CWs (i.e., B-CF2 treatment) with immobilized bacteria under saline conditions, which provides an alternative technology for the rapid removal of nitrogen from saline wastewater. [Abstract copyright: Copyright © 2021 Elsevier Ltd. All rights reserved.]
Keywords	Constructed wetlands, Immobilized microorganism technology, Nitrogen removal, Saline wastewater, Salt-tolerant microorganisms
Publisher	Elsevier
Journal	Environmental Pollution
ISSN	0269-7491
Online	18 Jun 2021
Print	15 Oct 2021
Publication process dates
Deposited	09 Jul 2021
Accepted	12 Jun 2021
Output status	Published
Digital Object Identifier (DOI)	https://doi.org/10.1016/j.envpol.2021.117592
Language	English

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