Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water

Article


Cheng, R., Wang, X., Zhu, H., Yan, B., Shutes, B., Xu, Y., Fu, B. and Wen, H. 2020. Isolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water. Scientific Reports. 10 (1). https://doi.org/10.1038/s41598-020-66989-5
TypeArticle
TitleIsolation and characterization of a salt-tolerant denitrifying bacterium Alishewanella sp. F2 from seawall muddy water
AuthorsCheng, R., Wang, X., Zhu, H., Yan, B., Shutes, B., Xu, Y., Fu, B. and Wen, H.
Abstract

A salt-tolerant denitrifying bacterium strain F2 was isolated from seawall muddy water in Dalian City, Liaoning Province, China. Strain F2 was identified by morphological observations, physiological and biochemical characteristics and 16 S rDNA identification. The salt tolerance of strain F2 was verified and the factors affecting the removal ability of strain F2 to nitrous nitrogen (NO2–N) and nitrate nitrogen (NO3–N) in saline conditions were investigated. Strain F2 was identified as Alishewanella sp., named Alishewanella sp. F2. Strain F2 can tolerate NaCl concentrations up to 70 g/L, and its most efficient denitrification capacity was observed at NaCl concentrations of 0−30 g/L. In the medium with NaCl concentrations of 0−30 g/L, strain F2 exhibited high removal efficiencies of NO2–N and NO3–N, with the removal percentages for both NO2–N and NO3–N of approximately 99%. In saline conditions with 30 g/L NaCl, the optimum culture pH, NaNO2 initial concentrations and inoculation sizes of strain F2 were 8−10, 0.4−0.8 g/L and 5−7%, respectively. Strain F2 was highly effective in removing NO2–N and NO3–N in saline conditions, and it has a good application potential in saline wastewater treatment.

LanguageEnglish
PublisherNature Publishing Group UK
JournalScientific Reports
ISSN2045-2322
Publication dates
Online19 Jun 2020
Print31 Dec 2020
Publication process dates
Deposited21 Jun 2021
Accepted01 Jun 2020
Output statusPublished
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© The Author(s) 2020.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Digital Object Identifier (DOI)https://doi.org/10.1038/s41598-020-66989-5
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