Degradation mechanism and toxicity reduction of methyl orange dye by a newly isolated bacterium Pseudomonas aeruginosa MZ520730
Article
Kishor, R., Purchase, D., Saratale, G., Ferreira, L., Hussain, C., Mulla, S. and Bharagava, R. 2021. Degradation mechanism and toxicity reduction of methyl orange dye by a newly isolated bacterium Pseudomonas aeruginosa MZ520730. Journal of Water Process Engineering. 43, pp. 1-10. https://doi.org/10.1016/j.jwpe.2021.102300
Type | Article |
---|---|
Title | Degradation mechanism and toxicity reduction of methyl orange dye by a newly isolated bacterium Pseudomonas aeruginosa MZ520730 |
Authors | Kishor, R., Purchase, D., Saratale, G., Ferreira, L., Hussain, C., Mulla, S. and Bharagava, R. |
Abstract | Methyl orange (MO) dye is recalcitrant in nature, hard to degrade and if released into the soil and aquatic resources could cause serious threats on environment and human health. MO is toxic to plant growth. Bacterial treatment may be a sustainable solution for its degradation and decolourization. In this work, a bacterium (RKS6) was isolated from textile industry wastewater and sludge samples and identified as Pseudomonas aeruginosa based on the 16S rRNA gene sequencing analysis. RKS6 showed more than 99% decolorization of MO dye (100 mg/l) and 96% reduction of total organic carbon (TOC) within 12 h, at 30 °C, pH 7 at static conditions. RKS6 also produced MnP enzyme of molecular weight ∼53 kDa as characterized by the SDS-PAGE analysis. Further, LC-MS analysis showed that MO dye was degraded into 4-[(4-aminophenyl) diazenyl] benzene sulfonate, 4, 2-((dihydroxymethyl) hyrazono-4) 5-benzene sulfonate, 4-(triazan-2-yl) benzene sulfonic, water and carbon dioxide by RKS6. Toxicity assessment showed that the solution treated by the bacterium allowed 90% seed germination indicating that RKS6 was effective in mineralization and detoxification of MO dye and can be effectively used in industrial wastewater treatment. |
Keywords | Methyl orange; Pseudomonas aeruginosa; Manganese peroxidase; Optimization; Biodegradation; Phytotoxicity |
Publisher | Elsevier |
Journal | Journal of Water Process Engineering |
ISSN | |
Electronic | 2214-7144 |
Publication dates | |
Online | 09 Sep 2021 |
01 Oct 2021 | |
Publication process dates | |
Submitted | 25 Jun 2021 |
Accepted | 30 Aug 2021 |
Deposited | 10 Sep 2021 |
Output status | Published |
Accepted author manuscript | License |
Copyright Statement | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jwpe.2021.102300 |
Scopus EID | 2-s2.0-85114601116 |
Web of Science identifier | WOS:000704632800013 |
Language | English |
https://repository.mdx.ac.uk/item/897q1
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