A study of bacteria producing carbonic anhydrase enzyme for CaCO3 precipitation and soil biocementation
Article
Meandira, W., Mavroulidou, M., Timmermans, M., Gunn, M., Gray, C., Pantoja Munoz, L. and Purchase, D. 2024. A study of bacteria producing carbonic anhydrase enzyme for CaCO3 precipitation and soil biocementation. Environmental Science and Pollution Research. 31 (33), pp. 45818-45833. https://doi.org/10.1007/s11356-024-34077-0
Type | Article |
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Title | A study of bacteria producing carbonic anhydrase enzyme for CaCO3 precipitation and soil biocementation |
Authors | Meandira, W., Mavroulidou, M., Timmermans, M., Gunn, M., Gray, C., Pantoja Munoz, L. and Purchase, D. |
Abstract | We study the carbonic anhydrase (CA) pathway using autochthonous CA-producing bacteria as a means of inducing calcite precipitation, which acts as a biocement to improve the engineering soil properties. Forty different microbial strains producing CA were isolated from the foundation soil of a railway embankment in Prickwillow, UK. Three of the best CA-producing strains were selected and identified by DNA sequencing as Bacillus licheniformis, Bacillus toyonensis and Bacillus pumilus with CA activity values respectively of 1.79 U/ml, 1.42 U/ml and 1.55 U/ml. To optimise the treatments, we investigated the effect of pH, temperature, zinc co-factor and cementation solution molarity on the growth and CA activity and bioprecipitates, with CO2 added in the form of bicarbonate. Scanning electron microscope (SEM) analysis of the bioprecipitates showed that these had characteristic morphologies of calcite and vaterite crystals. The formation of calcite was further corroborated by FT-IR and Raman analysis of bioprecipitates. The precultured bacteria were injected into the fine-grained soil together with cementation solution. Unconfined compressive strength in treated soil increased up to 1 MPa, and its calcium carbonate content increased by 2.78%. This, as well as the stability of the treated soil upon water immersion, proved the biocementation of the fine-grained soil. These findings suggest the potential of employing the CA biocementation route for soil stabilisation pending further development of the technique. |
Keywords | Carbonic anhydrase enzyme; Microbiological study; Calcite precipitation; Biocementation; Ground improvement |
Sustainable Development Goals | 12 Responsible consumption and production |
Middlesex University Theme | Sustainability |
Publisher | Springer |
Journal | Environmental Science and Pollution Research |
ISSN | 0944-1344 |
Electronic | 1614-7499 |
Publication dates | |
Online | 08 Jul 2024 |
Jul 2024 | |
Publication process dates | |
Submitted | 01 Feb 2024 |
Accepted | 18 Jun 2024 |
Deposited | 06 Aug 2024 |
Output status | Published |
Publisher's version | License File Access Level Open |
Copyright Statement | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s11356-024-34077-0 |
Language | English |
https://repository.mdx.ac.uk/item/16441x
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