Antibiotic selective pressure in microcosms: pollution influences the persistence of multidrug resistant Shigella flexneri 2a YSH6000 strain in polluted river water samples

Article


Maruzani, R., Pathak, A., Ward, M., Serafim, V., Pantoja Munoz, L., Shah, A. and Marvasi, M. 2020. Antibiotic selective pressure in microcosms: pollution influences the persistence of multidrug resistant Shigella flexneri 2a YSH6000 strain in polluted river water samples. Environmental Technology and Innovation. 19. https://doi.org/10.1016/j.eti.2020.100821
TypeArticle
TitleAntibiotic selective pressure in microcosms: pollution influences the persistence of multidrug resistant Shigella flexneri 2a YSH6000 strain in polluted river water samples
AuthorsMaruzani, R., Pathak, A., Ward, M., Serafim, V., Pantoja Munoz, L., Shah, A. and Marvasi, M.
Abstract

Some urban rivers reach dangerous concentrations of residual antibiotics imposing a certain level of selective pressure on microorganisms to develop various antibiotic resistance mechanisms. In the current work, we have measured the persistence and growth of a multidrug resistant strain of Shigella flexneri 2a YSH6000 under a mock release of lethal concentration of oxytetracycline in microcosms of River Thames water. The water was sampled from upstream (lower levels of pollution) and downstream (higher levels of pollution) of London city centre. In our in-vitro microcosms, in the presence of 160 μ g/mL of oxytetracycline, growth of S. flexneri in the downstream sector was up to 2 log(cfu/mL) higher relative to the upstream sector. This difference in growth is a sum of undefined interactions of different chemicals with the antibiotic. We extrapolated the contribution of two abundant pollutants in downstream sector: iron and phenanthrene. In the presence of selection pressure, iron at a concentration of 6.49 mg/L was found to foster the growth of resistant bacteria while phenanthrene at concentration of 160 μ g/L reduced the growth of the resistant strain. In addition, label free proteomics analysis showed that there are 64 proteins that were differentially expressed by the bacteria exposed to the upstream section versus the downstream sector. In the presence of oxytetracycline, at concentration of 160 μ g/mL, the differences reduced to only a few proteins, demonstrating that environmental stress impacts protein synthesis. Such mock studies contribute to our knowledge of chemicals that reduce growth of resistant strains and aids in the identification of selective biomarkers.

LanguageEnglish
PublisherElsevier
JournalEnvironmental Technology and Innovation
ISSN2352-1864
Publication dates
Online22 Apr 2020
Print31 Aug 2020
Publication process dates
Deposited23 Apr 2020
Accepted16 Apr 2020
Output statusPublished
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
License
Accepted author manuscript
Copyright Statement

© 2020. This author's accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Digital Object Identifier (DOI)https://doi.org/10.1016/j.eti.2020.100821
Permalink -

https://repository.mdx.ac.uk/item/88y3w

  • 48
    total views
  • 34
    total downloads
  • 3
    views this month
  • 0
    downloads this month

Export as

Related outputs

Molding the future: optimization of bioleaching of rare earth elements from electronic waste by Penicillium expansum and insights into its mechanism
Gonzalez Baez, A., Pantoja Munoz, L., Timmermans, M., Garelick, H. and Purchase, D. 2024. Molding the future: optimization of bioleaching of rare earth elements from electronic waste by Penicillium expansum and insights into its mechanism. Bioresource Technology. 402. https://doi.org/10.1016/j.biortech.2024.130750
Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia
Antia, I., Hills, F. and Shah, A. 2024. Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia. Analytical Methods. 16 (4), pp. 566-575. https://doi.org/10.1039/D3AY01578E
An assessment of different alkali-activated cements as stabilizers of sulfate-bearing soils
Mavroulidou, M., Gray, C., Pantoja Munoz, L. and Gunn, M. 2023. An assessment of different alkali-activated cements as stabilizers of sulfate-bearing soils. Quarterly Journal of Engineering Geology and Hydrogeology. 56 (2). https://doi.org/10.1144/qjegh2022-057
Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa
Shah, A., Shah, H., Duncan, L. and Ward, M. 2023. Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa. in: Shah, H., Gharbia, S., Shah, A., Tranfield, E. and Clive, T. (ed.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications Wiley. pp. 183-201
Progress in the microbiological applications of mass spectrometry: From electron impact to soft ionization techniques, MALDI-TOF MS and beyond
Shah, A., Shah, H., Gharbia, S. and Francese, S. 2023. Progress in the microbiological applications of mass spectrometry: From electron impact to soft ionization techniques, MALDI-TOF MS and beyond. in: Shah, H., Gharbia, S., Shah, A., Tranfield, E. and Clive, T. (ed.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications Wiley. pp. 1-35
A critical review of the quantification, analysis and detection of radionuclides in the environment using diffusive gradients in thin films (DGT): advances and perspectives
Pantoja Munoz, L. and Garelick, H. 2023. A critical review of the quantification, analysis and detection of radionuclides in the environment using diffusive gradients in thin films (DGT): advances and perspectives. Pure and Applied Chemistry. https://doi.org/10.1515/pac-2023-0809
Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov
Rudra, B., Duncan, L., Shah, A., Shah, H. and Gupta, R. 2022. Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov. International Journal of Systematic and Evolutionary Microbiology. 72 (11), pp. 1-15. https://doi.org/10.1099/ijsem.0.005542
Characterization of industrially pre-treated waste printed circuit boards for the potential recovery of rare earth elements
Gonzalez Baez, A., Pantoja Munoz, L., Garelick, H. and Purchase, D. 2022. Characterization of industrially pre-treated waste printed circuit boards for the potential recovery of rare earth elements. Environmental Technology and Innovation. 27. https://doi.org/10.1016/j.eti.2022.102481
Release of microplastic fibres and fragmentation to billions of nanoplastics from period products: preliminary assessment of potential health implications
Pantoja Munoz, L., Gonzalez Baez, A., Purchase, D., Jones, H. and Garelick, H. 2022. Release of microplastic fibres and fragmentation to billions of nanoplastics from period products: preliminary assessment of potential health implications. Environmental Science: Nano. 9 (2), pp. 606-620. https://doi.org/10.1039/d1en00755f
Natural based products for cleaning copper and copper alloys artefacts
Macchia, A., Colasanti, I., Rivaroli, L., Favero, G., de Caro, T., Pantoja Munoz, L., Campanella, L. and La Russa, M. 2023. Natural based products for cleaning copper and copper alloys artefacts. Natural Product Research. 37 (7), pp. 1177-1184. https://doi.org/10.1080/14786419.2021.2000408
Derivatisation methods for trace-level quantification of B-lactam antibiotics using HPLC
Shah, A., Orford, C., Perry, D. and Adlard, M. 1995. Derivatisation methods for trace-level quantification of B-lactam antibiotics using HPLC. LC-GC International. 6 (4), pp. 130-140.
Waste printed circuit boards (WPCBs) as a potential source for the recovery of rare earth elements through bioleaching
Gonzalez Baez, A., Pantoja Munoz, L., Garelick, H. and Purchase, D. 2021. Waste printed circuit boards (WPCBs) as a potential source for the recovery of rare earth elements through bioleaching. 17th International Conference on Environmental Science and Technology CEST2021. Athens, Greece 01 - 04 Sep 2021
Exploiting the efficacy of Tyro3 and folate receptors to enhance the delivery of gold nanoparticles into colorectal cancer cells in vitro
Patel, N., Ghali, L., Roitt, I., Pantoja Munoz, L. and Bayford, R. 2021. Exploiting the efficacy of Tyro3 and folate receptors to enhance the delivery of gold nanoparticles into colorectal cancer cells in vitro. Nanoscale Advances. 3 (18), pp. 5373-5386. https://doi.org/10.1039/d1na00318f
A bioleaching approach for the recovery of rare earth elements (REE) from waste printed circuit boards (WPCBs)
Gonzalez Baez, A., Pantoja Munoz, L., Garelick, H. and Purchase, D. 2021. A bioleaching approach for the recovery of rare earth elements (REE) from waste printed circuit boards (WPCBs). IUPAC 48th World Chemistry Congress. Montreal, Canada 13 - 20 Aug 2021
Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli
Yagnik, D., Ward, M. and Shah, A. 2021. Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli. Scientific Reports. 11 (1), pp. 1-7. https://doi.org/10.1038/s41598-020-78407-x
Recovery of rare earth elements from electronic waste through bioleaching
Gonzalez Baez, A., Pantoja Munoz, L., Garelick, H. and Purchase, D. 2020. Recovery of rare earth elements from electronic waste through bioleaching. LSBU 100 Women in Engineering - Engineering a World That Works For Everyone. London, UK 17 Nov 2020
Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry
Serafim, V., Shah, A., Licker, M., Horhat, F., Vulpie, S., Musuroi, C. and Muntean, D. 2020. Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry. Infection and Drug Resistance. 13, pp. 4021-4029. https://doi.org/10.2147/IDR.S267160
A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants
Marano, R., Fernandes, T., Manaia, C., Nunes, O., Morrison, D., Berendonk, T., Kreuzinger, N., Telson, T., Corno, G., Fatta-Kassinos, D., Merlin, C., Topp, E., Jurkevitch, E., Henn, L., Scott, A., Heß, S., Slipko, K., Laht, M., Kisand, V., Di Cesare, A., Karaolia, P., Michael, S., Petre, A., Rosal, R., Pruden, A., Riquelme, V., Agüera, A., Esteban, B., Luczkiewicz, A., Kalinowska, A., Leonard, A., Gaze, W., Adegoke, A., Stenstrom, T., Pollice, A., Salerno, C., Schwermer, C., Krzeminski, P., Guilloteau, H., Donner, E., Drigo, B., Libralato, G., Guida, M., Bürgmann, H., Beck, K., Garelick, H., Tacão, M., Henriques, I., Martínez-Alcalá, I., Guillén-Navarro, J., Popowska, M., Piotrowska, M., Quintela-Baluja, M., Bunce, J., Polo-López, M., Nahim-Granados, S., Pons, M., Milakovic, M., Udikovic-Kolic, N., Ory, J., Ousmane, T., Caballero, P., Oliver, A., Rodriguez-Mozaz, S., Balcazar, J., Jäger, T., Schwartz, T., Yang, Y., Zou, S., Lee, Y., Yoon, Y., Herzog, B., Mayrhofer, H., Prakash, O., Nimonkar, Y., Heath, E., Baraniak, A., Abreu-Silva, J., Choudhury, M., Pantoja Munoz, L., Krizanovic, S., Brunetti, G., Maile-Moskowitz, A., Brown, C. and Cytryn, E. 2020. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants. Environment International. 144, pp. 1-11. https://doi.org/10.1016/j.envint.2020.106035
MALDI TOF MS and currently related proteomic technologies in reconciling bacterial systematics
Shah, H., Shah, A., Belgacem, O., Ward, M., Dekio, I., Selami, L., Duncan, L., Bruce, K., Xu, Z., Mkrtchyan, H., Cave, R., Shah, L. and Gharbia, S. 2020. MALDI TOF MS and currently related proteomic technologies in reconciling bacterial systematics. in: Bridge, P., Smith, D. and Stackebrandt, E. (ed.) Trends in the Systematics of Bacteria and Fungi Wallingford, UK CAB International. pp. 93-118
SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000
Henríquez, T., Salazar, J., Marvasi, M., Shah, A., Corsini, G. and Toro, C. 2020. SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000. PLoS ONE. 15 (1), pp. 1-14. https://doi.org/10.1371/journal.pone.0228178
Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus
Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H. 2020. Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus. Proteomics. 20 (2), pp. 1-6. https://doi.org/10.1002/pmic.201900221
Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study
Singh, A., Zhao, K., Bell, C. and Shah, A. 2020. Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study. Rapid Communications in Mass Spectrometry. 34 (S4), pp. 1-12. https://doi.org/10.1002/rcm.8651
A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test
Marvasi, M., Canali, A., Perito, B., Shah, A. and Serafim, V. 2019. A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test. Journal of Microbiological Methods. 167, pp. 1-5. https://doi.org/10.1016/j.mimet.2019.105724
Characterisation of rare earth elements in waste printed circuit boards (WPCBs) and their bioleaching potential
Gonzalez Baez, A., Pantoja Munoz, L., Garelick, H. and Purchase, D. 2019. Characterisation of rare earth elements in waste printed circuit boards (WPCBs) and their bioleaching potential. 16th International Conference on Environmental Science and Technology. Rhodes, Greece 04 - 07 Sep 2019
Can quantification of Serum Glycans predict Pre-Eclampsia?
Antia, I., Shah, A., Yagnik, D., Syngelaki, A., Nicolaides, K. and Hills, F. 2018. Can quantification of Serum Glycans predict Pre-Eclampsia? International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e38-e39 https://doi.org/10.1016/j.placenta.2018.06.004
Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole
Shah, A., Adlard, M. and Holt, G. 1988. Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole. Analyst. 113 (8), pp. 1197-1200. https://doi.org/10.1039/an9881301197
Characterisation of "flushable" and "non-flushable" commercial wet wipes using microRaman, FTIR spectroscopy and fluorescence microscopy: to flush or not to flush
Pantoja Munoz, L., Gonzalez Baez, A., McKinney, D. and Garelick, H. 2018. Characterisation of "flushable" and "non-flushable" commercial wet wipes using microRaman, FTIR spectroscopy and fluorescence microscopy: to flush or not to flush. Environmental Science and Pollution Research. 25 (20), pp. 20268-20279. https://doi.org/10.1007/s11356-018-2400-9
Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion
Yagnik, D., Serafim, V., Choudhury, M. and Shah, A. 2017. Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion. International Conference on Infection Control and Clinical Microbiology. Chicago, USA 25 - 26 Sep 2017 SciTechnol. pp. 73 https://doi.org/10.4172/2329-9541-C1-006
A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization
Shah, A., Adlard, M. and Stride, J. 1990. A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization. Journal of Pharmaceutical and Biomedical Analysis. 8 (5), pp. 437-443. https://doi.org/10.1016/0731-7085(90)80072-W
pH regulation of penicillin production in Aspergillus nidulans
Shah, A., Tilburn, J., Adlard, M. and Arst, H. 1991. pH regulation of penicillin production in Aspergillus nidulans. FEMS microbiology letters. 77 (2-3), pp. 209-212. https://doi.org/10.1111/j.1574-6968.1991.tb04349.x
Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression
Yagnik, D., Serafin, V. and Shah, A. 2018. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression. Scientific Reports. 8 (1). https://doi.org/10.1038/s41598-017-18618-x
Effect of anthropogenic pollution on the fitness of tetracycline sensitive Shigella flexneri in Thames river water
Maruzani, R., Canali, A., Serafim, V., Pantoja Munoz, L., Shah, A., Perito, B. and Marvasi, M. 2018. Effect of anthropogenic pollution on the fitness of tetracycline sensitive Shigella flexneri in Thames river water. Journal of Environmental Chemical Engineering. 6 (1), pp. 19-27. https://doi.org/10.1016/j.jece.2017.11.069
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, I., Mathew, K., Yagnik, D., Hills, F. and Shah, A. 2018. Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. Analytical and Bioanalytical Chemistry. 410 (1), pp. 131-143. https://doi.org/10.1007/s00216-017-0703-1
Fitness analysis of tetracycline resistant and sensitive Shigella flexneri in lethal and sub-lethal concentrations of tetracycline in Thames river water microcosms
Maruzani, R., Shah, A., Pantoja Munoz, L. and Marvasi, M. 2017. Fitness analysis of tetracycline resistant and sensitive Shigella flexneri in lethal and sub-lethal concentrations of tetracycline in Thames river water microcosms. University Research Students Summer Conference 2017. London 2017
Evolutionary trends within the genus Acacia based on the accumulation of non-protein amino acids in seeds
Shah, A., Evans, C., Adlard, M. and Arce, M. 1994. Evolutionary trends within the genus Acacia based on the accumulation of non-protein amino acids in seeds. in: Sprent, J. and McKey, D. (ed.) Advances in Legume Systematics, Part 5: The Nitrogen Factor London, UK The Royal Botanic Gardens, Kew.
High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds
Shah, A., Younie, D., Adlard, M. and Evans, C. 1992. High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds. Phytochemical Analysis. 3 (1), pp. 20-25. https://doi.org/10.1002/pca.2800030104
Non-protein amino acids in seeds of neotropical species of acacia
Evans, C., Shah, A., Adlard, M. and Arce, M. 1992. Non-protein amino acids in seeds of neotropical species of acacia. Phytochemistry. 32 (1), pp. 123-126. https://doi.org/10.1016/0031-9422(92)80116-V
Development of colorimetric method for the detection of amines bound to solid support
Shah, A., de Biasi, V., Camilleri, P. and Rahman, S. 1997. Development of colorimetric method for the detection of amines bound to solid support. Analytical Communications. 34 (11), pp. 325-328. https://doi.org/10.1039/A705630C
Development of a spectrophotometric method for the measurement of thiols at trace levels
Shah, A., de Biasi, V. and Camilleri, P. 1995. Development of a spectrophotometric method for the measurement of thiols at trace levels. Analytical Proceedings including Analytical Communications. 32 (4), pp. 149-153. https://doi.org/10.1039/AI9953200149
Capillary electrophoresis in the pharmaceutical industry: applications in discovery and chemical development
Hadley, M., Gilges, M., Senior, J., Shah, A. and Camilleri, P. 2000. Capillary electrophoresis in the pharmaceutical industry: applications in discovery and chemical development. Journal of Chromatography B: Biomedical Sciences and Applications. 745 (1), pp. 177-188. https://doi.org/10.1016/S0378-4347(00)00153-5
Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates
Shah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P. 1999. Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. Journal of Chromatography B: Biomedical Sciences and Applications. 735 (2), pp. 133-140. https://doi.org/10.1016/S0378-4347(99)00403-X
Orexin A activates locus coeruleus cell firing and increases arousal in the rat
Hagan, J., Leslie, R., Patel, S., Evans, M., Wattam, T., Holmes, S., Benham, C., Taylor, S., Routledge, C., Hemmati, P., Munton, R., Ashmeade, T., Shah, A., Hatcher, J., Hatcher, P., Jones, D., Smith, M., Piper, D., Hunter, A., Porter, R. and Upton, N. 1999. Orexin A activates locus coeruleus cell firing and increases arousal in the rat. Proceedings of the National Academy of Sciences. 96 (19), pp. 10911-10916. https://doi.org/10.1073/pnas.96.19.10911
Amino acid neurotransmitters: separation approaches and diagnostic value
Shah, A., Crespi, F. and Heidbreder, C. 2002. Amino acid neurotransmitters: separation approaches and diagnostic value. Journal of Chromatography B. 781 (1-2), pp. 151-163. https://doi.org/10.1016/S1570-0232(02)00621-9
High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
Hows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates. Journal of Neuroscience Methods. 137 (2), pp. 221-226. https://doi.org/10.1016/j.jneumeth.2004.02.011
High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples
Hows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. Journal of Neuroscience Methods. 138 (1-2), pp. 123-132. https://doi.org/10.1016/j.jneumeth.2004.03.021
Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns
Dawson, L., Organ, A., Winter, P., Lacroix, L., Shilliam, C., Heidbreder, C. and Shah, A. 2004. Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns. Journal of Chromatography B. 807 (2), pp. 235-241. https://doi.org/10.1016/j.jchromb.2004.04.012
Selective antagonism at dopamine D3 receptors enhances monoaminergic and cholinergic neurotransmission in the rat anterior cingulate cortex
Lacroix, L., Hows, M., Shah, A., Hagan, J. and Heidbreder, C. 2003. Selective antagonism at dopamine D3 receptors enhances monoaminergic and cholinergic neurotransmission in the rat anterior cingulate cortex. Neuropsychopharmacology. 28, pp. 839-849. https://doi.org/10.1038/sj.npp.1300114
High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates
Hows, M., Organ, A., Murray, S., Dawson, L., Foxton, R., Heidbreder, C., Hughes, Z., Lacroix, L. and Shah, A. 2002. High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates. Journal of Neuroscience Methods. 121 (1), pp. 33-39. https://doi.org/10.1016/S0165-0270(02)00228-5
Increased responsiveness of dopamine to atypical, but not typical antipsychotics in the medial prefrontal cortex of rats reared in isolation
Heidbreder, C., Foxton, R., Cilia, J., Hughes, Z., Shah, A., Atkins, A., Hunter, J., Hagan, J. and Jones, D. 2001. Increased responsiveness of dopamine to atypical, but not typical antipsychotics in the medial prefrontal cortex of rats reared in isolation. Psychopharmacology. 156 (2-3), pp. 338-351. https://doi.org/10.1007/s002130100760
Development of a high-pressure gradient pumping system for parallel liquid chromatography/mass spectrometry for the analysis of combinatorial libraries
Tolson, D., Organ, A. and Shah, A. 2001. Development of a high-pressure gradient pumping system for parallel liquid chromatography/mass spectrometry for the analysis of combinatorial libraries. Rapid Communications in Mass Spectrometry. 15 (14), pp. 1244-1249. https://doi.org/10.1002/rcm.359
The evaluation of interdigitated array electrodes for the measurement of catecholamines and indoleamines
Senior, J., Shah, A., Monteux, C. and de Biasi, V. 2001. The evaluation of interdigitated array electrodes for the measurement of catecholamines and indoleamines. Journal of Pharmaceutical and Biomedical Analysis. 24 (5-6), pp. 843-848. https://doi.org/10.1016/S0731-7085(00)00551-3
Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain
Heidbreder, C., Lacroix, L., Atkins, A., Organ, A., Murray, S., West, A. and Shah, A. 2001. Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain. Journal of Neuroscience Methods. 112 (2), pp. 135-144. https://doi.org/10.1016/S0165-0270(01)00460-5
Determination of β-lactams and their biosynthetic intermediates in fermentation media by pre-column derivatisation followed by fluorescence detection
Shah, A. and Adlard, M. 1988. Determination of β-lactams and their biosynthetic intermediates in fermentation media by pre-column derivatisation followed by fluorescence detection. Journal of Chromatography B: Biomedical Sciences and Applications. 424, pp. 325-336. https://doi.org/10.1016/S0378-4347(00)81109-3
Evaluation of expression and function of the H+/myo-inositol transporter HMIT;
Di Daniel, E., Mok, S., Mead, E., Mutinelli, C., Zambello, E., Caberlotto, L., Pell, T., Langmead, C., Shah, A., Duddy, G., Kew, J. and Maycox, P. 2009. Evaluation of expression and function of the H+/myo-inositol transporter HMIT; BMC Cell Biology. 10. https://doi.org/10.1186/1471-2121-10-54
Elucidating the intra-species proteotypes of pseudomonas aeruginosa from cystic fibrosis
Olkun, A., Shah, A. and Shah, H. 2017. Elucidating the intra-species proteotypes of pseudomonas aeruginosa from cystic fibrosis. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 579-592
Subtyping of Staphylococcus spp. Based upon MALDI-TOF MS data analysis
Xu, Z., Olkun, A., Vranckx, K., Mkrtchyan, H., Shah, A., Pot, B., Cutler, R. and Shah, H. 2017. Subtyping of Staphylococcus spp. Based upon MALDI-TOF MS data analysis. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 563-578
Determination of antimicrobial resistance using tandem mass spectrometry
Shah, A., Serafim, V., Xu, Z., Mkrtchyan, H. and Shah, H. 2017. Determination of antimicrobial resistance using tandem mass spectrometry. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 399-418
Microbial DNA analysis by MALDI-TOF mass spectrometry
Honisch, C., Serafim, V., Hennessy, N., Allen, D., Ring, C., Pantoja Munoz, L., Gharbia, S., Shah, A. and Shah, H. 2017. Microbial DNA analysis by MALDI-TOF mass spectrometry. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford Wiley. pp. 187-209
A study of the inhibition of biofilm formation on multiple surfaces by a biosurfactant containing four discrete lipopeptides
Khonsari, S., Gonzalez Baez, A., Nguyen, H., Pantoja Munoz, L. and Purchase, D. 2017. A study of the inhibition of biofilm formation on multiple surfaces by a biosurfactant containing four discrete lipopeptides. Microbiology Society Annual Conference. Edinburgh, United Kingdom 03 - 06 Apr 2017
Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis
Serafim, V., Shah, A., Puiu, M., Andreescu, N., Coricovac, D., Nosyrev, A., Spandidos, D., Tsatsakis, A., Dehelean, C. and Pinzaru, I. 2017. Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis. International Journal of Molecular Medicine. 40 (4), pp. 1096-1104. https://doi.org/10.3892/ijmm.2017.3083
Rapid identification of E. coli bacteriophages using mass spectrometry
Serafim, V., Ring, C., Pantoja Munoz, L., Shah, H. and Shah, A. 2017. Rapid identification of E. coli bacteriophages using mass spectrometry. Journal of Proteomics and Enzymology. 6 (1), pp. 1-5. https://doi.org/10.4172/2470-1289.1000130
Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring
Antia, I., Yagnik, D., Pantoja Munoz, L., Shah, A. and Hills, F. 2017. Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry. 530, pp. 17-30. https://doi.org/10.1016/j.ab.2017.04.019
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris
Pantoja Munoz, L., Purchase, D., Jones, H., Raab, A., Urgast, D., Feldmann, J. and Garelick, H. 2016. The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris. Aquatic Toxicology. 175, pp. 56-72. https://doi.org/10.1016/j.aquatox.2016.02.020
Therapeutic potential of delivering arsenic trioxide into HPV-infected cervical cancer cells using liposomal nanotechnology
Wang, X., Li, D., Ghali, L., Xia, R., Pantoja Munoz, L., Garelick, H., Bell, C. and Wen, S. 2016. Therapeutic potential of delivering arsenic trioxide into HPV-infected cervical cancer cells using liposomal nanotechnology. Nanoscale Research Letters. 11 (1), pp. 1-8. https://doi.org/10.1186/s11671-016-1307-y
Consideration of the bioavailability of metal/metalloid species in freshwaters: experiences regarding the implementation of biotic ligand model-based approaches in risk assessment frameworks
Rüdel, H., Diaz Muñiz, C., Garelick, H., Kandile, N., Miller, B., Pantoja Munoz, L., Peijnenburg, W., Purchase, D., Shevah, Y., Van Sprang, P., Vijer, M. and Vink, J. 2015. Consideration of the bioavailability of metal/metalloid species in freshwaters: experiences regarding the implementation of biotic ligand model-based approaches in risk assessment frameworks. Environmental Science and Pollution Research. 22 (10), pp. 7405-7421. https://doi.org/10.1007/s11356-015-4257-5
Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry
Sniecinska-Cooper, A., Shah, A., Dimitriou, D., Iles, R., Butler, S. and Bayford, R. 2015. Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry. Journal of Chromatography B. 978-79, pp. 18-23. https://doi.org/10.1016/j.jchromb.2014.11.016
The role of vitamin In type 2 diabetes
Ali, A., Appiah, S., Shah, A. and Ghali, L. 2014. The role of vitamin In type 2 diabetes. Middlesex University Postgraduate Summer Conference. London, UK 19 Jun 2014
The mechanisms of arsenic detoxification by the green microalgae chlorella vulgaris
Pantoja Munoz, L. 2014. The mechanisms of arsenic detoxification by the green microalgae chlorella vulgaris. PhD thesis Middlesex University Science and Technology
The mechanisms of arsenic bioremediation from water by the green microalgae Chlorella vulgaris
Pantoja Munoz, L., Purchase, D., Jones, H., Feldmann, J. and Garelick, H. 2014. The mechanisms of arsenic bioremediation from water by the green microalgae Chlorella vulgaris. THE BNASS / TraceSpec Tandem Conference. Aberdeen, Scotland, UK 31 Aug - 04 Sep 2014
The removal of arsenate from water using iron-modified diatomite (D-Fe): isotherm and column experiments
Pantoja Munoz, L., Jones, H., Garelick, H., Mohamedbakr, H. and Burkitbayev, M. 2014. The removal of arsenate from water using iron-modified diatomite (D-Fe): isotherm and column experiments. Environmental Science and Pollution Research. 21 (1), pp. 495-506. https://doi.org/10.1007/s11356-013-1891-7
Enhanced determination of As-phytochelatin complexes in Chlorella vulgaris using focused sonication for extraction of water-soluble species
Pantoja Munoz, L., Purchase, D., Jones, H., Feldmann, J. and Garelick, H. 2014. Enhanced determination of As-phytochelatin complexes in Chlorella vulgaris using focused sonication for extraction of water-soluble species. Analytical Methods. 6 (3), pp. 791-797. https://doi.org/10.1039/C3AY41629A
Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats
Bianchi, M., Fone, K., Shah, A., Atkins, A., Dawson, L., Heidbreder, C., Hagan, J. and Marsden, C. 2009. Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats. European Neuropsychopharmacology. 19 (11), pp. 778-790. https://doi.org/10.1016/j.euroneuro.2009.06.005
A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples
Shah, A. 2013. A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples. Journal of Chromatography A.
Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus
Bianchi, M., Shah, A., Fone, K., Atkins, A., Dawson, L., Heidbreder, C., Hows, M., Hagan, J. and Marsden, C. 2009. Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus. Synapse. 63 (4), pp. 359-364. https://doi.org/10.1002/syn.20614
Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR
Fricker, A., Mok, M., De la Flor, R., Shah, A., Woolley, M., Dawson, L. and Kew, J. 2009. Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR. Neuropharmacology. 56 (6-7), pp. 1060-1067. https://doi.org/10.1016/j.neuropharm.2009.03.002
Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts
Shah, A., De la Flor, R., Atkins, A., Slone-Murphy, J. and Dawson, L. 2008. Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. Journal of Chromatography B. 876 (2), pp. 153-158. https://doi.org/10.1016/j.jchromb.2008.10.012
Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine
Trivedi, D., Jones, H., Shah, A. and Iles, R. 2012. Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine. Journal of Chromatography & Separation Techniques. 03 (06). https://doi.org/10.4172/2157-7064.1000144