Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa

Book chapter

Duncan, L., Shah, A., Ward, M., Gupta, R.S., Rudra, B., Han, A., Bruce, K. and Shah, H.N. 2023. Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa. in: Shah, H.N., Gharbia, S.E., Shah, A., Tranfield, E.Y. and Thompson, K.C. (ed.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications Wiley. pp. 183-209
Chapter titleDiversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa
AuthorsDuncan, L., Shah, A., Ward, M., Gupta, R.S., Rudra, B., Han, A., Bruce, K. and Shah, H.N.
Abstract

In 1956, Williamson [1] contested the singular morphological description of Pseudomonas aeruginosa presented in Bergey’s Manual of Determinative Bacteriology, Such a limited description was proposed as inadequate at describing this diverse bacterial species. Traditional phenotypical methods for bacterial identification would reveal the phenotypic diversity in P. aeruginosa isolates, with the isolation of at least six colony types from different samples [2, 3]. Further variability would be unveiled in the morphologies, pigments, and substrate use, with reports of ‘atypical’ isolates. Nonetheless, these atypical traits were detected more and more frequently and instead pointed towards an extremely diverse species. This chapter examines the diversity of P. aeruginosa and its close relationship with the proteome. We discuss the cutting-edge mass spectrometry (MS) techniques that are continuing to reveal the extent and impact of proteomic diversity with respect to transmission and human infections of P. aeruginosa. In a parallel study, we undertake phylogenomic analysis of the species based upon its core protein sequences against eight other Pseudomonas species that may help to elucidate molecular markers that distinguish subtypes.

Keywordsantibiotic resistance; environmental transmission; mass spectrometry; phenotype; proteome; Pseudomonas aeruginosa
Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
Research GroupBiomarkers for Cancer group
Page range183-209
Book titleMicrobiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications
EditorsShah, H.N., Gharbia, S.E., Shah, A., Tranfield, E.Y. and Thompson, K.C.
PublisherWiley
ISBN
Hardcover9781119814054
Electronic9781119814085
Electronic9781119814078
Publication dates
Print18 Apr 2023
Online31 May 2023
Publication process dates
Deposited09 Mar 2023
AcceptedApr 2023
Output statusPublished
Additional information

Chapter 7

Digital Object Identifier (DOI)https://doi.org/10.1002/9781119814085.ch7
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Is part ofhttps://www.wiley.com/en-ie/Microbiological+Identification+using+MALDI+TOF+and+Tandem+Mass+Spectrometry:+Industrial+and+Environmental+Applications-p-9781119814054
Is part ofhttps://doi.org/10.1002/9781119814085
LanguageEnglish
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