A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test

Article

Marvasi, M., Canali, A., Perito, B., Shah, A. and Serafim, V. 2019. A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test. Journal of Microbiological Methods. 167, pp. 1-5. https://doi.org/10.1016/j.mimet.2019.105724
TypeArticle
TitleA method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test
AuthorsMarvasi, M., Canali, A., Perito, B., Shah, A. and Serafim, V.
Abstract

Antibiotics released in the environment exert a selective pressure on the resident microbiota. It is well accepted that the mere measurement of antibiotics does not reflect the actual bioavailability. In fact, antibiotics can be adsorbed or complexed to particles and/or chemicals in water and soil. Bioavailable concentrations of antibiotics in soil and water are subjected to great uncertainty, therefore biological assays are increasingly recognized as that allow an indirect determination of the residual antibiotic activity. Here we propose how a fitness test for bacteria can be used to qualitatively assess the bioavailability of a specific antibiotic in the environment. The findings show that by using a pair of resistant and sensitive bacterial strains, the resulting fitness can indirectly reflect antibiotic bioavailability. Hence, this test can be used as a complementary assay to other biological and
chemical tests to assess bioavailability of antibiotics.

PublisherElsevier
JournalJournal of Microbiological Methods
ISSN0167-7012
Publication dates
Online06 Nov 2019
Print01 Dec 2019
Publication process dates
Deposited05 Nov 2019
Accepted15 Sep 2019
Output statusPublished
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Copyright Statement

© 2019. This author's accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Digital Object Identifier (DOI)https://doi.org/10.1016/j.mimet.2019.105724
LanguageEnglish
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