High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates

Article

Hows, M., Organ, A., Murray, S., Dawson, L., Foxton, R., Heidbreder, C., Hughes, Z., Lacroix, L. and Shah, A. 2002. High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates. Journal of Neuroscience Methods. 121 (1), pp. 33-39. https://doi.org/10.1016/S0165-0270(02)00228-5
TypeArticle
TitleHigh-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates
AuthorsHows, M., Organ, A., Murray, S., Dawson, L., Foxton, R., Heidbreder, C., Hughes, Z., Lacroix, L. and Shah, A.
Abstract

A high-throughput liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the analysis of acetylcholine (ACh) in brain dialysates. This separation of ACh is based on cation exchange chromatography with elution buffer consisting of a mixture of ammonium acetate, ammonium formate and acetonitrile. Using isocratic separation conditions, ACh was resolved within a minute and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for ACh was found to be 1 fmol on column with a S/N ratio of 3:1. The assay has been used routinely for the measurement of ACh in brain dialysates from awake freely moving rats. Furthermore, separation conditions were modified to allow simultaneous measurement of ACh and the acetylcholine esterase inhibitor, neostigmine.

PublisherElsevier
JournalJournal of Neuroscience Methods
ISSN0165-0270
Publication dates
Print15 Nov 2002
Online01 Oct 2002
Publication process dates
Deposited02 Nov 2017
Accepted26 Jul 2002
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/S0165-0270(02)00228-5
LanguageEnglish
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