Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates

Article

Shah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P. 1999. Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. Journal of Chromatography B: Biomedical Sciences and Applications. 735 (2), pp. 133-140. https://doi.org/10.1016/S0378-4347(99)00403-X
TypeArticle
TitleDevelopment of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates
AuthorsShah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P.
Abstract

An automated precolumn derivatisation method has been developed for the measurement of fourteen amino acids in brain tissue and microdialysate samples. The method involves labelling amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide (CN-). The resulting highly stable N-substituted 1-cyanobenz[f]isoindole (CBI) derivatives were separated using a binary gradient elution profile and detected fluorometrically. The order of elution of the derivatised amino acids was confirmed by using liquid chromatography with fluorescence and mass spectrometric detection in tandem. Linear calibration plots were obtained for all amino acids in the range studied (0.2-12.5 microM). The limit of detection for CBI derivatives of amino acids was in the range 5-20 fmol (S/N=2) using a 5 microl injection volume. The method has been used for the measurement of amino acids in microdialysates from rat brain and tissue homogenates from different regions of mouse brain.

PublisherElsevier
JournalJournal of Chromatography B: Biomedical Sciences and Applications
ISSN1570-0232
Publication dates
Online29 Nov 1999
Print10 Dec 1999
Publication process dates
Deposited03 Nov 2017
Submitted13 Apr 1999
Accepted30 Aug 1999
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/S0378-4347(99)00403-X
LanguageEnglish
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