Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates
Article
Shah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P. 1999. Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. Journal of Chromatography B: Biomedical Sciences and Applications. 735 (2), pp. 133-140. https://doi.org/10.1016/S0378-4347(99)00403-X
Type | Article |
---|---|
Title | Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates |
Authors | Shah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P. |
Abstract | An automated precolumn derivatisation method has been developed for the measurement of fourteen amino acids in brain tissue and microdialysate samples. The method involves labelling amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide (CN-). The resulting highly stable N-substituted 1-cyanobenz[f]isoindole (CBI) derivatives were separated using a binary gradient elution profile and detected fluorometrically. The order of elution of the derivatised amino acids was confirmed by using liquid chromatography with fluorescence and mass spectrometric detection in tandem. Linear calibration plots were obtained for all amino acids in the range studied (0.2-12.5 microM). The limit of detection for CBI derivatives of amino acids was in the range 5-20 fmol (S/N=2) using a 5 microl injection volume. The method has been used for the measurement of amino acids in microdialysates from rat brain and tissue homogenates from different regions of mouse brain. |
Publisher | Elsevier |
Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
ISSN | 1570-0232 |
Publication dates | |
Online | 29 Nov 1999 |
10 Dec 1999 | |
Publication process dates | |
Deposited | 03 Nov 2017 |
Submitted | 13 Apr 1999 |
Accepted | 30 Aug 1999 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1016/S0378-4347(99)00403-X |
Language | English |
https://repository.mdx.ac.uk/item/87459
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