High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

Article

Hows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. Journal of Neuroscience Methods. 138 (1-2), pp. 123-132. https://doi.org/10.1016/j.jneumeth.2004.03.021
TypeArticle
TitleHigh-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples
AuthorsHows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A.
Abstract

A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively.

PublisherElsevier
JournalJournal of Neuroscience Methods
ISSN0165-0270
Publication dates
Online19 Jun 2004
Print30 Sep 2004
Publication process dates
Deposited03 Nov 2017
Accepted26 Mar 2004
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jneumeth.2004.03.021
LanguageEnglish
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