High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples
Article
Hows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. Journal of Neuroscience Methods. 138 (1-2), pp. 123-132. https://doi.org/10.1016/j.jneumeth.2004.03.021
Type | Article |
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Title | High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples |
Authors | Hows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A. |
Abstract | A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively. |
Publisher | Elsevier |
Journal | Journal of Neuroscience Methods |
ISSN | 0165-0270 |
Publication dates | |
Online | 19 Jun 2004 |
30 Sep 2004 | |
Publication process dates | |
Deposited | 03 Nov 2017 |
Accepted | 26 Mar 2004 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jneumeth.2004.03.021 |
Language | English |
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