Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine
Article
Trivedi, D., Jones, H., Shah, A. and Iles, R. 2012. Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine. Journal of Chromatography & Separation Techniques. 03 (06). https://doi.org/10.4172/2157-7064.1000144
Type | Article |
---|---|
Title | Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine |
Authors | Trivedi, D., Jones, H., Shah, A. and Iles, R. |
Abstract | Urine is a product of the body’s metabolism and the majority of the metabolic products exiting via the renal system are rendered polar in order to be water soluble. Resolution of urinary metabolites for metabolomic studies requires the development of HPLC separation techniques that match this feature of biological chemistry. ZIC –HILIC is an ideal candidate to take forward resolution of such metabolites where reverse phase is unable to give adequate separation. Metabolomic data has to be processed by Shotgun multivariate analysis to sift through thousands of analytes and their variables such as ion intensity. |
Keywords | Urinary metabolomics; ZIC HILIC-MS; Method development; Shotgun analysis |
Research Group | Biomarkers for Cancer group |
Reproductive Biology group | |
Publisher | OMICS Publishing Group |
Journal | Journal of Chromatography & Separation Techniques |
ISSN | 2155-7064 |
Publication dates | |
Oct 2012 | |
Publication process dates | |
Deposited | 04 Jul 2013 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.4172/2157-7064.1000144 |
Language | English |
File |
https://repository.mdx.ac.uk/item/84255
Download files
84
total views31
total downloads0
views this month0
downloads this month