Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus

Article

Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H. 2020. Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus. Proteomics. 20 (2), pp. 1-6. https://doi.org/10.1002/pmic.201900221
TypeArticle
TitleComparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus
AuthorsXu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H.
Abstract

Purpose: Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non-healthcare environments.
Experiment design: Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected.
Results: 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up-regulated and 156 were down-regulated. Proteins that had differences in abundance were predominantly involved in catalytic and binding activity. Among 164 differently abundant proteins, 29 were involved in pathogenesis, antimicrobial resistance，stress response, mismatch repair and cell wall synthesis. Twenty-two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up-regulated in MRSA. Moreover, the up-regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host.
Conclusions: Enhanced pathogenicity, antimicrobial resistance and stress response were observed in MRSA compared to MSSA.

KeywordsBiochemistry, Molecular Biology
PublisherWiley-VCH Verlag
JournalProteomics
ISSN1615-9853
Electronic1615-9861
Publication dates
Online16 Jan 2020
Print16 Jan 2020
Publication process dates
Deposited19 Dec 2019
Accepted18 Dec 2019
Output statusPublished
Accepted author manuscript
Copyright Statement

This is the peer reviewed version of the following article: Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R., Mkrtchyan, H. V., Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus. Proteomics 2020, 20, 1900221, which has been published in final form at https://doi.org/10.1002/pmic.201900221. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Digital Object Identifier (DOI)https://doi.org/10.1002/pmic.201900221
LanguageEnglish
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