Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

Article

Shah, A., De la Flor, R., Atkins, A., Slone-Murphy, J. and Dawson, L. 2008. Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. Journal of Chromatography B. 876 (2), pp. 153-158. https://doi.org/10.1016/j.jchromb.2008.10.012
TypeArticle
TitleDevelopment and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts
AuthorsShah, A., De la Flor, R., Atkins, A., Slone-Murphy, J. and Dawson, L.
Abstract

A liquid chromatography-tandem mass spectrometric method has been developed for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate. The analytes were separated within 5 min using an anion exchange/reverse phase column. The lower limit of quantification for Glu, NAA and NAAG was found to be 5, 50 and 6 nM, respectively, with a signal-to-noise ratio of 5:1. Using this methodology the basal levels of Glu, NAA and NAAG could be measured consistently in in vitro superfusion samples from rat hippocampus. The assay was also used for measurement of the distribution of Glu, NAA and NAAG in different regions of the rat brain.

KeywordsN-Acetylaspartic acid; N-Acetylaspartylglutamic acid; glutamic acid; liquid chromatography–tandem mass spectrometry; superfusion; Ex vivo
Research GroupBiomarkers for Cancer group
Reproductive Biology group
PublisherElsevier
JournalJournal of Chromatography B
ISSN1570-0232
Publication dates
Online14 Oct 2008
Print15 Dec 2008
Publication process dates
Deposited04 Jul 2013
Accepted08 Oct 2008
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jchromb.2008.10.012
LanguageEnglish
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