Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Article
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
Type | Article |
---|---|
Title | Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor |
Authors | Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. |
Abstract | This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting. |
Research Group | Biophysics and Bioengineering group |
Publisher | Elsevier |
Journal | Biosensors and Bioelectronics |
ISSN | 0956-5663 |
Publication dates | |
Online | 28 Oct 2016 |
15 Jun 2017 | |
Publication process dates | |
Deposited | 08 Nov 2016 |
Accepted | 25 Oct 2016 |
Output status | Published |
Accepted author manuscript | License |
Copyright Statement | © 2016. This author's accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.bios.2016.10.077 |
Language | English |
https://repository.mdx.ac.uk/item/86v5y
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