Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study

Article


Singh, A., Zhao, K., Bell, C. and Shah, A. 2020. Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study. Rapid Communications in Mass Spectrometry. 34 (S4), pp. 1-12. https://doi.org/10.1002/rcm.8651
TypeArticle
TitleEffect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study
AuthorsSingh, A., Zhao, K., Bell, C. and Shah, A.
Abstract

Rationale: Patients with type 2 diabetes may co-ingest herbal and prescription medicine to control their blood sugar levels. Competitive binding of drug and herb may mutually affect their metabolism. This can alter the level of drug and its kinetics in the body, potentially causing toxicities or loss of efficacy. Understanding how metabolism of sulfonylureas like glyburide and gliclazide can be affected by the presence of berberine and vice versa can provide valuable information on the possible risk of toxicities caused by co-ingestion of drugs.
Methods: Berberine and sulfonylureas (glyburide and gliclazide) were co-incubated with rat liver microsomes in the presence of NADPH regenerating system. The metabolites of berberine and sulfonylureas were analysed using liquid chromatography with high resolution mass spectrometry in the positive ion mode. The role of individual isozymes in the metabolism of berberine, glyburide and gliclazide was investigated by using specific inhibitors.
Results: In vitro metabolism of berberine lead to the formation of demethyleneberberine (B1a) and B1b through demethylenation. Berberrubine (B2a) and its isomer (B2b) was formed through demethylation. The isozymes CYP3A and CYP2D were found to be involved in the metabolism of berberine. In vitro metabolism of glyburide and gliclazide lead to the formation of hydroxylated metabolites. The isozymes CYP3A and CYP2C were found to be involved in the metabolism of glyburide. Gliclazide was metabolised by CYP2C. In vitro co-incubation of glyburide or gliclazide with berberine showed that each drugs metabolism was compromised as both share a common isozymes. A strong negative linear correlation of glyburide or gliclazide metabolites levels and the concentration of berberine confirmed the effect of berberine on the metabolism of sulfonylureas

KeywordsOrganic Chemistry, Analytical Chemistry, Spectroscopy
PublisherWiley
JournalRapid Communications in Mass Spectrometry
ISSN0951-4198
Electronic1097-0231
Publication dates
Online04 Mar 2020
Print09 Sep 2020
Publication process dates
Deposited06 Nov 2019
Accepted04 Nov 2019
Submitted28 Jun 2019
Output statusPublished
Accepted author manuscript
File Access Level
Open
Copyright Statement

This is the peer reviewed version of the following article: Singh, A, Zhao, K, Bell, C, Shah, AJ. Effect of Berberine on In Vitro Metabolism of Sulfonylureas: A herb‐drug interactions study. Rapid Commun Mass Spectrom. 2020; 34( S4):e8651, which has been published in final form at https://doi.org/10.1002/rcm.8651. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Additional information

Special Issue research article
Volume34, IssueS4 - Special Issue: Dai Games

Digital Object Identifier (DOI)https://doi.org/10.1002/rcm.8651
Web of Science identifierWOS:000561965700001
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/88925

Download files


Accepted author manuscript
RCM-19-0223.R2_Proof_hi.pdf
File access level: Open

  • 45
    total views
  • 10
    total downloads
  • 3
    views this month
  • 3
    downloads this month

Export as

Related outputs

Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia
Antia, I., Hills, F. and Shah, A. 2024. Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia. Analytical Methods. 16 (4), pp. 566-575. https://doi.org/10.1039/D3AY01578E
Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa
Shah, A., Shah, H., Duncan, L. and Ward, M. 2023. Diversity, transmission and selective pressure on the proteome of Pseudomonas aeruginosa. in: Shah, H., Gharbia, S., Shah, A., Tranfield, E. and Clive, T. (ed.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications Wiley. pp. 183-201
Progress in the microbiological applications of mass spectrometry: From electron impact to soft ionization techniques, MALDI-TOF MS and beyond
Shah, A., Shah, H., Gharbia, S. and Francese, S. 2023. Progress in the microbiological applications of mass spectrometry: From electron impact to soft ionization techniques, MALDI-TOF MS and beyond. in: Shah, H., Gharbia, S., Shah, A., Tranfield, E. and Clive, T. (ed.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Industrial and Environmental Applications Wiley. pp. 1-35
Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov
Rudra, B., Duncan, L., Shah, A., Shah, H. and Gupta, R. 2022. Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov. International Journal of Systematic and Evolutionary Microbiology. 72 (11), pp. 1-15. https://doi.org/10.1099/ijsem.0.005542
Derivatisation methods for trace-level quantification of B-lactam antibiotics using HPLC
Shah, A., Orford, C., Perry, D. and Adlard, M. 1995. Derivatisation methods for trace-level quantification of B-lactam antibiotics using HPLC. LC-GC International. 6 (4), pp. 130-140.
Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli
Yagnik, D., Ward, M. and Shah, A. 2021. Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli. Scientific Reports. 11 (1), pp. 1-7. https://doi.org/10.1038/s41598-020-78407-x
Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry
Serafim, V., Shah, A., Licker, M., Horhat, F., Vulpie, S., Musuroi, C. and Muntean, D. 2020. Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry. Infection and Drug Resistance. 13, pp. 4021-4029. https://doi.org/10.2147/IDR.S267160
MALDI TOF MS and currently related proteomic technologies in reconciling bacterial systematics
Shah, H., Shah, A., Belgacem, O., Ward, M., Dekio, I., Selami, L., Duncan, L., Bruce, K., Xu, Z., Mkrtchyan, H., Cave, R., Shah, L. and Gharbia, S. 2020. MALDI TOF MS and currently related proteomic technologies in reconciling bacterial systematics. in: Bridge, P., Smith, D. and Stackebrandt, E. (ed.) Trends in the Systematics of Bacteria and Fungi Wallingford, UK CAB International. pp. 93-118
Antibiotic selective pressure in microcosms: pollution influences the persistence of multidrug resistant Shigella flexneri 2a YSH6000 strain in polluted river water samples
Maruzani, R., Pathak, A., Ward, M., Serafim, V., Pantoja Munoz, L., Shah, A. and Marvasi, M. 2020. Antibiotic selective pressure in microcosms: pollution influences the persistence of multidrug resistant Shigella flexneri 2a YSH6000 strain in polluted river water samples. Environmental Technology and Innovation. 19. https://doi.org/10.1016/j.eti.2020.100821
SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000
Henríquez, T., Salazar, J., Marvasi, M., Shah, A., Corsini, G. and Toro, C. 2020. SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000. PLoS ONE. 15 (1), pp. 1-14. https://doi.org/10.1371/journal.pone.0228178
Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus
Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R. and Mkrtchyan, H. 2020. Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus. Proteomics. 20 (2), pp. 1-6. https://doi.org/10.1002/pmic.201900221
A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test
Marvasi, M., Canali, A., Perito, B., Shah, A. and Serafim, V. 2019. A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test. Journal of Microbiological Methods. 167, pp. 1-5. https://doi.org/10.1016/j.mimet.2019.105724
Can quantification of Serum Glycans predict Pre-Eclampsia?
Antia, I., Shah, A., Yagnik, D., Syngelaki, A., Nicolaides, K. and Hills, F. 2018. Can quantification of Serum Glycans predict Pre-Eclampsia? International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e38-e39 https://doi.org/10.1016/j.placenta.2018.06.004
Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole
Shah, A., Adlard, M. and Holt, G. 1988. Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole. Analyst. 113 (8), pp. 1197-1200. https://doi.org/10.1039/an9881301197
Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion
Yagnik, D., Serafim, V., Choudhury, M. and Shah, A. 2017. Apple cider vinegar (ACV®) displays antimicrobial activity directly against Escherichia coli, Staphylococcus aureus and Candida albican proteins and in vitro monocytes exposed to microbes by inhibiting inflammatory cytokine secretion. International Conference on Infection Control and Clinical Microbiology. Chicago, USA 25 - 26 Sep 2017 SciTechnol. pp. 73 https://doi.org/10.4172/2329-9541-C1-006
A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization
Shah, A., Adlard, M. and Stride, J. 1990. A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization. Journal of Pharmaceutical and Biomedical Analysis. 8 (5), pp. 437-443. https://doi.org/10.1016/0731-7085(90)80072-W
pH regulation of penicillin production in Aspergillus nidulans
Shah, A., Tilburn, J., Adlard, M. and Arst, H. 1991. pH regulation of penicillin production in Aspergillus nidulans. FEMS microbiology letters. 77 (2-3), pp. 209-212. https://doi.org/10.1111/j.1574-6968.1991.tb04349.x
Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression
Yagnik, D., Serafin, V. and Shah, A. 2018. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression. Scientific Reports. 8 (1). https://doi.org/10.1038/s41598-017-18618-x
Effect of anthropogenic pollution on the fitness of tetracycline sensitive Shigella flexneri in Thames river water
Maruzani, R., Canali, A., Serafim, V., Pantoja Munoz, L., Shah, A., Perito, B. and Marvasi, M. 2018. Effect of anthropogenic pollution on the fitness of tetracycline sensitive Shigella flexneri in Thames river water. Journal of Environmental Chemical Engineering. 6 (1), pp. 19-27. https://doi.org/10.1016/j.jece.2017.11.069
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, I., Mathew, K., Yagnik, D., Hills, F. and Shah, A. 2018. Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. Analytical and Bioanalytical Chemistry. 410 (1), pp. 131-143. https://doi.org/10.1007/s00216-017-0703-1
Fitness analysis of tetracycline resistant and sensitive Shigella flexneri in lethal and sub-lethal concentrations of tetracycline in Thames river water microcosms
Maruzani, R., Shah, A., Pantoja Munoz, L. and Marvasi, M. 2017. Fitness analysis of tetracycline resistant and sensitive Shigella flexneri in lethal and sub-lethal concentrations of tetracycline in Thames river water microcosms. University Research Students Summer Conference 2017. London 2017
Evolutionary trends within the genus Acacia based on the accumulation of non-protein amino acids in seeds
Shah, A., Evans, C., Adlard, M. and Arce, M. 1994. Evolutionary trends within the genus Acacia based on the accumulation of non-protein amino acids in seeds. in: Sprent, J. and McKey, D. (ed.) Advances in Legume Systematics, Part 5: The Nitrogen Factor London, UK The Royal Botanic Gardens, Kew.
High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds
Shah, A., Younie, D., Adlard, M. and Evans, C. 1992. High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds. Phytochemical Analysis. 3 (1), pp. 20-25. https://doi.org/10.1002/pca.2800030104
Non-protein amino acids in seeds of neotropical species of acacia
Evans, C., Shah, A., Adlard, M. and Arce, M. 1992. Non-protein amino acids in seeds of neotropical species of acacia. Phytochemistry. 32 (1), pp. 123-126. https://doi.org/10.1016/0031-9422(92)80116-V
Development of colorimetric method for the detection of amines bound to solid support
Shah, A., de Biasi, V., Camilleri, P. and Rahman, S. 1997. Development of colorimetric method for the detection of amines bound to solid support. Analytical Communications. 34 (11), pp. 325-328. https://doi.org/10.1039/A705630C
Development of a spectrophotometric method for the measurement of thiols at trace levels
Shah, A., de Biasi, V. and Camilleri, P. 1995. Development of a spectrophotometric method for the measurement of thiols at trace levels. Analytical Proceedings including Analytical Communications. 32 (4), pp. 149-153. https://doi.org/10.1039/AI9953200149
Capillary electrophoresis in the pharmaceutical industry: applications in discovery and chemical development
Hadley, M., Gilges, M., Senior, J., Shah, A. and Camilleri, P. 2000. Capillary electrophoresis in the pharmaceutical industry: applications in discovery and chemical development. Journal of Chromatography B: Biomedical Sciences and Applications. 745 (1), pp. 177-188. https://doi.org/10.1016/S0378-4347(00)00153-5
Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates
Shah, A., de Biasi, V., Taylor, S., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C. and Camilleri, P. 1999. Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. Journal of Chromatography B: Biomedical Sciences and Applications. 735 (2), pp. 133-140. https://doi.org/10.1016/S0378-4347(99)00403-X
Orexin A activates locus coeruleus cell firing and increases arousal in the rat
Hagan, J., Leslie, R., Patel, S., Evans, M., Wattam, T., Holmes, S., Benham, C., Taylor, S., Routledge, C., Hemmati, P., Munton, R., Ashmeade, T., Shah, A., Hatcher, J., Hatcher, P., Jones, D., Smith, M., Piper, D., Hunter, A., Porter, R. and Upton, N. 1999. Orexin A activates locus coeruleus cell firing and increases arousal in the rat. Proceedings of the National Academy of Sciences. 96 (19), pp. 10911-10916. https://doi.org/10.1073/pnas.96.19.10911
Amino acid neurotransmitters: separation approaches and diagnostic value
Shah, A., Crespi, F. and Heidbreder, C. 2002. Amino acid neurotransmitters: separation approaches and diagnostic value. Journal of Chromatography B. 781 (1-2), pp. 151-163. https://doi.org/10.1016/S1570-0232(02)00621-9
High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
Hows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates. Journal of Neuroscience Methods. 137 (2), pp. 221-226. https://doi.org/10.1016/j.jneumeth.2004.02.011
High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples
Hows, M., Lacroix, L., Heidbreder, C., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. Journal of Neuroscience Methods. 138 (1-2), pp. 123-132. https://doi.org/10.1016/j.jneumeth.2004.03.021
Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns
Dawson, L., Organ, A., Winter, P., Lacroix, L., Shilliam, C., Heidbreder, C. and Shah, A. 2004. Rapid high-throughput assay for the measurement of amino acids from microdialysates and brain tissue using monolithic C18-bonded reversed-phase columns. Journal of Chromatography B. 807 (2), pp. 235-241. https://doi.org/10.1016/j.jchromb.2004.04.012
Selective antagonism at dopamine D3 receptors enhances monoaminergic and cholinergic neurotransmission in the rat anterior cingulate cortex
Lacroix, L., Hows, M., Shah, A., Hagan, J. and Heidbreder, C. 2003. Selective antagonism at dopamine D3 receptors enhances monoaminergic and cholinergic neurotransmission in the rat anterior cingulate cortex. Neuropsychopharmacology. 28, pp. 839-849. https://doi.org/10.1038/sj.npp.1300114
High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates
Hows, M., Organ, A., Murray, S., Dawson, L., Foxton, R., Heidbreder, C., Hughes, Z., Lacroix, L. and Shah, A. 2002. High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates. Journal of Neuroscience Methods. 121 (1), pp. 33-39. https://doi.org/10.1016/S0165-0270(02)00228-5
Increased responsiveness of dopamine to atypical, but not typical antipsychotics in the medial prefrontal cortex of rats reared in isolation
Heidbreder, C., Foxton, R., Cilia, J., Hughes, Z., Shah, A., Atkins, A., Hunter, J., Hagan, J. and Jones, D. 2001. Increased responsiveness of dopamine to atypical, but not typical antipsychotics in the medial prefrontal cortex of rats reared in isolation. Psychopharmacology. 156 (2-3), pp. 338-351. https://doi.org/10.1007/s002130100760
Development of a high-pressure gradient pumping system for parallel liquid chromatography/mass spectrometry for the analysis of combinatorial libraries
Tolson, D., Organ, A. and Shah, A. 2001. Development of a high-pressure gradient pumping system for parallel liquid chromatography/mass spectrometry for the analysis of combinatorial libraries. Rapid Communications in Mass Spectrometry. 15 (14), pp. 1244-1249. https://doi.org/10.1002/rcm.359
The evaluation of interdigitated array electrodes for the measurement of catecholamines and indoleamines
Senior, J., Shah, A., Monteux, C. and de Biasi, V. 2001. The evaluation of interdigitated array electrodes for the measurement of catecholamines and indoleamines. Journal of Pharmaceutical and Biomedical Analysis. 24 (5-6), pp. 843-848. https://doi.org/10.1016/S0731-7085(00)00551-3
Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain
Heidbreder, C., Lacroix, L., Atkins, A., Organ, A., Murray, S., West, A. and Shah, A. 2001. Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain. Journal of Neuroscience Methods. 112 (2), pp. 135-144. https://doi.org/10.1016/S0165-0270(01)00460-5
Determination of β-lactams and their biosynthetic intermediates in fermentation media by pre-column derivatisation followed by fluorescence detection
Shah, A. and Adlard, M. 1988. Determination of β-lactams and their biosynthetic intermediates in fermentation media by pre-column derivatisation followed by fluorescence detection. Journal of Chromatography B: Biomedical Sciences and Applications. 424, pp. 325-336. https://doi.org/10.1016/S0378-4347(00)81109-3
Evaluation of expression and function of the H+/myo-inositol transporter HMIT;
Di Daniel, E., Mok, S., Mead, E., Mutinelli, C., Zambello, E., Caberlotto, L., Pell, T., Langmead, C., Shah, A., Duddy, G., Kew, J. and Maycox, P. 2009. Evaluation of expression and function of the H+/myo-inositol transporter HMIT; BMC Cell Biology. 10. https://doi.org/10.1186/1471-2121-10-54
Elucidating the intra-species proteotypes of pseudomonas aeruginosa from cystic fibrosis
Olkun, A., Shah, A. and Shah, H. 2017. Elucidating the intra-species proteotypes of pseudomonas aeruginosa from cystic fibrosis. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 579-592
Subtyping of Staphylococcus spp. Based upon MALDI-TOF MS data analysis
Xu, Z., Olkun, A., Vranckx, K., Mkrtchyan, H., Shah, A., Pot, B., Cutler, R. and Shah, H. 2017. Subtyping of Staphylococcus spp. Based upon MALDI-TOF MS data analysis. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 563-578
Determination of antimicrobial resistance using tandem mass spectrometry
Shah, A., Serafim, V., Xu, Z., Mkrtchyan, H. and Shah, H. 2017. Determination of antimicrobial resistance using tandem mass spectrometry. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford, UK Wiley. pp. 399-418
Microbial DNA analysis by MALDI-TOF mass spectrometry
Honisch, C., Serafim, V., Hennessy, N., Allen, D., Ring, C., Pantoja Munoz, L., Gharbia, S., Shah, A. and Shah, H. 2017. Microbial DNA analysis by MALDI-TOF mass spectrometry. in: Shah, H. and Gharbia, S. (ed.) MALDI-TOF and Tandem MS for Clinical Microbiology Oxford Wiley. pp. 187-209
Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis
Serafim, V., Shah, A., Puiu, M., Andreescu, N., Coricovac, D., Nosyrev, A., Spandidos, D., Tsatsakis, A., Dehelean, C. and Pinzaru, I. 2017. Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis. International Journal of Molecular Medicine. 40 (4), pp. 1096-1104. https://doi.org/10.3892/ijmm.2017.3083
Rapid identification of E. coli bacteriophages using mass spectrometry
Serafim, V., Ring, C., Pantoja Munoz, L., Shah, H. and Shah, A. 2017. Rapid identification of E. coli bacteriophages using mass spectrometry. Journal of Proteomics and Enzymology. 6 (1), pp. 1-5. https://doi.org/10.4172/2470-1289.1000130
Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring
Antia, I., Yagnik, D., Pantoja Munoz, L., Shah, A. and Hills, F. 2017. Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry. 530, pp. 17-30. https://doi.org/10.1016/j.ab.2017.04.019
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry
Sniecinska-Cooper, A., Shah, A., Dimitriou, D., Iles, R., Butler, S. and Bayford, R. 2015. Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry. Journal of Chromatography B. 978-79, pp. 18-23. https://doi.org/10.1016/j.jchromb.2014.11.016
The role of vitamin In type 2 diabetes
Ali, A., Appiah, S., Shah, A. and Ghali, L. 2014. The role of vitamin In type 2 diabetes. Middlesex University Postgraduate Summer Conference. London, UK 19 Jun 2014
Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats
Bianchi, M., Fone, K., Shah, A., Atkins, A., Dawson, L., Heidbreder, C., Hagan, J. and Marsden, C. 2009. Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats. European Neuropsychopharmacology. 19 (11), pp. 778-790. https://doi.org/10.1016/j.euroneuro.2009.06.005
A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples
Shah, A. 2013. A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples. Journal of Chromatography A.
Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus
Bianchi, M., Shah, A., Fone, K., Atkins, A., Dawson, L., Heidbreder, C., Hows, M., Hagan, J. and Marsden, C. 2009. Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus. Synapse. 63 (4), pp. 359-364. https://doi.org/10.1002/syn.20614
Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR
Fricker, A., Mok, M., De la Flor, R., Shah, A., Woolley, M., Dawson, L. and Kew, J. 2009. Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR. Neuropharmacology. 56 (6-7), pp. 1060-1067. https://doi.org/10.1016/j.neuropharm.2009.03.002
Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts
Shah, A., De la Flor, R., Atkins, A., Slone-Murphy, J. and Dawson, L. 2008. Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. Journal of Chromatography B. 876 (2), pp. 153-158. https://doi.org/10.1016/j.jchromb.2008.10.012
Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine
Trivedi, D., Jones, H., Shah, A. and Iles, R. 2012. Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine. Journal of Chromatography & Separation Techniques. 03 (06). https://doi.org/10.4172/2157-7064.1000144