Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study

Article

Singh, A., Zhao, K., Bell, C. and Shah, A. 2020. Effect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study. Rapid Communications in Mass Spectrometry. 34 (S4), pp. 1-12. https://doi.org/10.1002/rcm.8651
TypeArticle
TitleEffect of Berberine on in vitro metabolism of Sulfonylureas: a herb-drug interactions study
AuthorsSingh, A., Zhao, K., Bell, C. and Shah, A.
Abstract

Rationale: Patients with type 2 diabetes may co-ingest herbal and prescription medicine to control their blood sugar levels. Competitive binding of drug and herb may mutually affect their metabolism. This can alter the level of drug and its kinetics in the body, potentially causing toxicities or loss of efficacy. Understanding how metabolism of sulfonylureas like glyburide and gliclazide can be affected by the presence of berberine and vice versa can provide valuable information on the possible risk of toxicities caused by co-ingestion of drugs.
Methods: Berberine and sulfonylureas (glyburide and gliclazide) were co-incubated with rat liver microsomes in the presence of NADPH regenerating system. The metabolites of berberine and sulfonylureas were analysed using liquid chromatography with high resolution mass spectrometry in the positive ion mode. The role of individual isozymes in the metabolism of berberine, glyburide and gliclazide was investigated by using specific inhibitors.
Results: In vitro metabolism of berberine lead to the formation of demethyleneberberine (B1a) and B1b through demethylenation. Berberrubine (B2a) and its isomer (B2b) was formed through demethylation. The isozymes CYP3A and CYP2D were found to be involved in the metabolism of berberine. In vitro metabolism of glyburide and gliclazide lead to the formation of hydroxylated metabolites. The isozymes CYP3A and CYP2C were found to be involved in the metabolism of glyburide. Gliclazide was metabolised by CYP2C. In vitro co-incubation of glyburide or gliclazide with berberine showed that each drugs metabolism was compromised as both share a common isozymes. A strong negative linear correlation of glyburide or gliclazide metabolites levels and the concentration of berberine confirmed the effect of berberine on the metabolism of sulfonylureas

KeywordsOrganic Chemistry, Analytical Chemistry, Spectroscopy
PublisherWiley
JournalRapid Communications in Mass Spectrometry
ISSN0951-4198
Electronic1097-0231
Publication dates
Online04 Mar 2020
Print09 Sep 2020
Publication process dates
Deposited06 Nov 2019
Accepted04 Nov 2019
Submitted28 Jun 2019
Output statusPublished
Accepted author manuscript
File Access Level
Open
Copyright Statement

This is the peer reviewed version of the following article: Singh, A, Zhao, K, Bell, C, Shah, AJ. Effect of Berberine on In Vitro Metabolism of Sulfonylureas: A herb‐drug interactions study. Rapid Commun Mass Spectrom. 2020; 34( S4):e8651, which has been published in final form at https://doi.org/10.1002/rcm.8651. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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Special Issue research article
Volume34, IssueS4 - Special Issue: Dai Games

Digital Object Identifier (DOI)https://doi.org/10.1002/rcm.8651
Web of Science identifierWOS:000561965700001
LanguageEnglish
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Appiah, S., Revitt, D., Jones, H., Vu, M., Simmonds, M. and Bell, C. 2017. Antiinflammatory and hepatoprotective medicinal herbs as potential substitutes for bear bile. in: Zeng, B. and Zhao, K. (ed.) Neurobiology of Chinese Herb Medicine Elsevier.
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
Recent advances in arsenic trioxide encapsulated nanoparticles as drug delivery agents to solid cancers
Akhtar, A., Wang, S., Ghali, L., Bell, C. and Wen, S. 2017. Recent advances in arsenic trioxide encapsulated nanoparticles as drug delivery agents to solid cancers. Journal of Biomedical Research. 31 (3), pp. 177-188. https://doi.org/10.7555/JBR.31.20160059
Therapeutic potential of delivering arsenic trioxide into HPV-infected cervical cancer cells using liposomal nanotechnology
Wang, X., Li, D., Ghali, L., Xia, R., Pantoja Munoz, L., Garelick, H., Bell, C. and Wen, S. 2016. Therapeutic potential of delivering arsenic trioxide into HPV-infected cervical cancer cells using liposomal nanotechnology. Nanoscale Research Letters. 11 (1), pp. 1-8. https://doi.org/10.1186/s11671-016-1307-y
Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry
Sniecinska-Cooper, A., Shah, A., Dimitriou, D., Iles, R., Butler, S. and Bayford, R. 2015. Determination of urinary cortisol, cortisone and 6-sulfatoxymelatonin using dilute and shoot ultra-high pressure liquid chromatography-tandem mass spectrometry. Journal of Chromatography B. 978-79, pp. 18-23. https://doi.org/10.1016/j.jchromb.2014.11.016
The role of vitamin In type 2 diabetes
Ali, A., Appiah, S., Shah, A. and Ghali, L. 2014. The role of vitamin In type 2 diabetes. Middlesex University Postgraduate Summer Conference. London, UK 19 Jun 2014
Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus
Bianchi, M., Shah, A., Fone, K., Atkins, A., Dawson, L., Heidbreder, C., Hows, M., Hagan, J. and Marsden, C. 2009. Fluoxetine administration modulates the cytoskeletal microtubular system in the rat hippocampus. Synapse. 63 (4), pp. 359-364. https://doi.org/10.1002/syn.20614
Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR
Fricker, A., Mok, M., De la Flor, R., Shah, A., Woolley, M., Dawson, L. and Kew, J. 2009. Effects of N-acetylaspartylglutamate (NAAG) at group II mGluRs and NMDAR. Neuropharmacology. 56 (6-7), pp. 1060-1067. https://doi.org/10.1016/j.neuropharm.2009.03.002
Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts
Shah, A., De la Flor, R., Atkins, A., Slone-Murphy, J. and Dawson, L. 2008. Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. Journal of Chromatography B. 876 (2), pp. 153-158. https://doi.org/10.1016/j.jchromb.2008.10.012
Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine
Trivedi, D., Jones, H., Shah, A. and Iles, R. 2012. Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) metabolomics method for Shotgun analysis of human urine. Journal of Chromatography & Separation Techniques. 03 (06). https://doi.org/10.4172/2157-7064.1000144
A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples
Shah, A. 2013. A high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous measurement of acetylcholine and clozapine in microdialysis samples. Journal of Chromatography A.
Herbalism and Osteoarthritis; methodological aspects of a small research study investigating herbal treatment outcomes, patient and practitioner viewpoints.
Bell, L., Bell, C., Chevallier, A., McDermott, A. and Adams, R. 1999. Herbalism and Osteoarthritis; methodological aspects of a small research study investigating herbal treatment outcomes, patient and practitioner viewpoints. British Sociological Association, Medical Sociology Group Conference. York, UK 24 - 26 Sep 1999
Herbal treatment for osteoarthritis: a pilot study investigating outcomes.
Bell, C., Bell, L., Chevallier, A. and Adams, R. 2003. Herbal treatment for osteoarthritis: a pilot study investigating outcomes. 10th Annual Symposium on Complementary Health Care. London, UK 21 - 22 Nov 2003
Investigation of the safety and efficacy of Chinese herbs traditionally used to treat inflammatory diseases
Appiah, S., Kokubun, T., Bremner, P., Heinrich, M., Bell, C. and Simmonds, M. 2003. Investigation of the safety and efficacy of Chinese herbs traditionally used to treat inflammatory diseases. 10th Annual Symposium on Complementary Health Care. London, UK 21 - 22 Nov 2003
Evaluation of the antioxidant activity of selected non-food plants traditionally used in the treatment of arthritis.
Pendry, B., Busia, K. and Bell, C. 2003. Evaluation of the antioxidant activity of selected non-food plants traditionally used in the treatment of arthritis. 10th Annual Symposium on Complementary Health Care. London, UK 21 - 22 Nov 2003
A celebration of ten years of successful international cooperation and exchange between Beijing University of Chinese Medicine and Middlesex University in London.
Bell, C. 2007. A celebration of ten years of successful international cooperation and exchange between Beijing University of Chinese Medicine and Middlesex University in London. World symposium on TCM accupuncture & moxibustion: present and future of the Chinese traditional medicine in Europe.. Tarragona, Spain 22 - 24 Jun 2007 pp. 1-13
Herbal alternatives to bear bile: effects of scutellaria baicalensis Georgi on IL-6 promoter and CYP3A4 activities.
Appiah, S., Bremner, P., Kokubun, T., Russell, M., Bell, C., Heinrich, M. and Simmonds, M. 2006. Herbal alternatives to bear bile: effects of scutellaria baicalensis Georgi on IL-6 promoter and CYP3A4 activities. 13th Annual Symposium on Complementary Health Care. Exeter, UK 12 - 14 Dec 2006
Phytochemical Evaluation of Selected Anti-oxidant-Containing Medicinal Plants for Use in the Preparation of a herbal Formula – a Preliminary Study.
Pendry, B., Busia, K. and Bell, C. 2005. Phytochemical Evaluation of Selected Anti-oxidant-Containing Medicinal Plants for Use in the Preparation of a herbal Formula – a Preliminary Study. Chemistry and Biodiversity. 2 (7), pp. 917-922. https://doi.org/10.1002/cbdv.200590068
Plant substances as alternatives for animal products in traditional medicines.
Bell, C., Simmonds, M., Appiah, S. and Howes, M. 2006. Plant substances as alternatives for animal products in traditional medicines. London Department for the Environment, Food and Rural Affairs.
Evaluation of student experience on an overseas placement: Students of Traditional Chinese Medicine on clinical placement at hospitals affiliated to Beijing University of Chinese Medicine.
Bell, C. and Cunningham, S. 2006. Evaluation of student experience on an overseas placement: Students of Traditional Chinese Medicine on clinical placement at hospitals affiliated to Beijing University of Chinese Medicine. Journal of Health, Social and Environmental Issues. 7 (1), pp. 59-68.
Current influences on traditional Chinese medicine education in the UK: the experience of a collaborative programme between Middlesex University and Beijing University of chinese medicine.
Bell, C. and Cheng, M. 2005. Current influences on traditional Chinese medicine education in the UK: the experience of a collaborative programme between Middlesex University and Beijing University of chinese medicine. Journal of the Association of Traditional Chinese Medicine.
Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats
Bianchi, M., Fone, K., Shah, A., Atkins, A., Dawson, L., Heidbreder, C., Hagan, J. and Marsden, C. 2009. Chronic fluoxetine differentially modulates the hippocampal microtubular and serotonergic system in grouped and isolation reared rats. European Neuropsychopharmacology. 19 (11), pp. 778-790. https://doi.org/10.1016/j.euroneuro.2009.06.005
Follicular fluid levels of inhibin A, inhibin B, and activin A levels reflect changes in follicle size but are not independent markers of the oocyte's ability to fertilize
Wen, S., Tozer, A., Butler, S., Bell, C., Docherty, S. and Iles, R. 2006. Follicular fluid levels of inhibin A, inhibin B, and activin A levels reflect changes in follicle size but are not independent markers of the oocyte's ability to fertilize. Fertility and Sterility. 85 (6), pp. 1723-1729. https://doi.org/10.1016/j.fertnstert.2005.11.058