High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds

Article

Shah, A., Younie, D., Adlard, M. and Evans, C. 1992. High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds. Phytochemical Analysis. 3 (1), pp. 20-25. https://doi.org/10.1002/pca.2800030104
TypeArticle
TitleHigh performance liquid chromatography of non-protein amino acids extracted from Acacia seeds
AuthorsShah, A., Younie, D., Adlard, M. and Evans, C.
Abstract

A sensitive, reversed phase, high performance liquid chromatographic procedure for the analysis of non-protein amino acids has been developed. The method is based on precolumn derivatization with 9-fluoroenylmethyl chloroformate of the non-protein amino acids characteristic of seeds of Acacia species, namely N-acetyldjenkolic acid, diaminopropionic acid and diaminobutyric acid and their acetyl, ureido and oxalyl N-substituted derivatives. The separation of derivatives was achieved by means of a gradient elution programme utilizing two buffers of different pH values and acetonitrile. Following separation, the derivatized amino acids were detected spectrofluorometrically. The levels of non-protein amino acids in seeds of Acacia species, which were representative of three taxonomically distinct groups, were measured by analysis of ethanol extracts.

PublisherWiley
JournalPhytochemical Analysis
ISSN0958-0344
Electronic1099-1565
Publication dates
Print31 Jan 1992
Online02 Mar 2007
Publication process dates
Deposited10 Nov 2017
Accepted12 Oct 1991
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1002/pca.2800030104
LanguageEnglish
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