Probing AMR mechanisms through MS analysis of the proteome of key human pathogens

Book chapter

Shah, A.J., Asif, A., Espinoza, C., Rani, A.B., Farhan, I. and Shah, H.N. 2026. Probing AMR mechanisms through MS analysis of the proteome of key human pathogens. in: Shah, H.N., Mkrtchyan, H.V., Raptakis, E., Belgacem, O. and Gharbia, S. (ed.) Mass Spectrometry and Spectroscopy in Microbiology: Identification, Subtyping and Antimicrobial Resistance Detection Wiley.
Chapter titleProbing AMR mechanisms through MS analysis of the proteome of key human pathogens
AuthorsShah, A.J., Asif, A., Espinoza, C., Rani, A.B., Farhan, I. and Shah, H.N.
Abstract

Antimicrobial resistance is an escalating global threat that demands a deeper understanding of bacterial responses to antibiotic stress. This chapter describes the application of label-free quantitative proteomics to investigate resistance- and antibiotic-induced changes in protein expression across clinically relevant Gram-positive and Gram-negative pathogens. Comparative proteomic analysis of methicillin-resistant and methicillin-sensitive Staphylococcus aureus identified protein expression changes associated with resistance and cellular adaptation. Proteomic profiling of a multidrug-resistant Escherichia coli strain treated with cefotaxime at the minimum inhibitory concentration revealed regulation of resistance-associated proteins and stress-response pathways linked to survival under β-lactam exposure. In Pseudomonas aeruginosa, proteome responses to tobramycin were examined in both clinical and reference strains, revealing strain-specific adaptive mechanisms. Additionally, mutant P. aeruginosa strains PvA and PvB were compared with the PAO1 reference strain to identify proteomic alterations associated with genetic variation. Collectively, these studies demonstrate the value of proteomics in elucidating mechanisms underlying antimicrobial resistance and antibiotic response.

Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
Book titleMass Spectrometry and Spectroscopy in Microbiology: Identification, Subtyping and Antimicrobial Resistance Detection
EditorsShah, H.N., Mkrtchyan, H.V., Raptakis, E., Belgacem, O. and Gharbia, S.
PublisherWiley
ISBN
Hardcover9781394358137
Publication dates
Print26 Nov 2026
Publication process dates
Accepted02 Jun 2026
Deposited08 Jun 2026
Output statusIn press
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Is part ofhttps://www.wiley.com/en-jp/shop/general-chemistry/mass-spectrometry-and-spectroscopy-in-microbiology-identification-subtyping-and-antimicrobial-resistance-detection-p-9781394358137
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