Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole
Article
Shah, A., Adlard, M. and Holt, G. 1988. Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole. Analyst. 113 (8), pp. 1197-1200. https://doi.org/10.1039/an9881301197
Type | Article |
---|---|
Title | Determination of natural penicillins in fermentation media by high-performance liquid chromatography using pre-column derivatisation with 1-hydroxybenzotriazole |
Authors | Shah, A., Adlard, M. and Holt, G. |
Abstract | The separation and determination of trace amounts of 6-aminopenicillanic acid (6-APAJ and penicillins G, V,X, K and penicillin G penicilloic acid in fermentation broths has been achieved using pre-columnderivatisation with 1 -hydroxybenzotriazole. Fermentation broth samples containing penicillins were mixedwith benzoic anhydride and left to stand at room temperature for 3 min. The latter step was essential for thestabilisation of derivatives of amino group-containing penicillins. The resulting mixtures were then treated |
Publisher | Royal Society of Chemistry |
Journal | Analyst |
ISSN | 0003-2654 |
Publication dates | |
22 Aug 1988 | |
Publication process dates | |
Deposited | 02 Jul 2018 |
Accepted | 09 Apr 1988 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1039/an9881301197 |
Language | English |
https://repository.mdx.ac.uk/item/87v33
62
total views0
total downloads4
views this month0
downloads this month