A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization

Article

Shah, A., Adlard, M. and Stride, J. 1990. A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization. Journal of Pharmaceutical and Biomedical Analysis. 8 (5), pp. 437-443. https://doi.org/10.1016/0731-7085(90)80072-W
TypeArticle
TitleA sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization
AuthorsShah, A., Adlard, M. and Stride, J.
Abstract

Precolumn derivatization procedures using 1,2,4-triazole for the detection and quantitation of sulbactam and clavulanic acid spiked into urine and blood serum at trace levels have been developed. Sulbactam and clavulanic acid produced derivatives which absorbed maximally at 325 and 315 nm, respectively. The methods allow the detection of clavulanic acid and sulbactam down to 0.05 micrograms ml-1 in serum and 0.5 micrograms ml-1 in urine. The relative standard deviation for five replicate analyses of sulbactam and clavulanic acid at a concentration of 20 micrograms ml-1 in serum and urine ranged from 2-6%. In further HPLC experiments with sulbactam in phosphate buffer solution, ampicillin was found as a contaminant (0.5% by mass) in the sulbactam sample provided. The significance of this finding is discussed

PublisherElsevier
JournalJournal of Pharmaceutical and Biomedical Analysis
ISSN0731-7085
Publication dates
Print27 Jun 1990
Publication process dates
Deposited02 Feb 2018
Accepted28 Feb 1990
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/0731-7085(90)80072-W
LanguageEnglish
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