Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia
Article
Antia, I., Hills, F. and Shah, A. 2024. Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia. Analytical Methods. 16 (4), pp. 566-575. https://doi.org/10.1039/D3AY01578E
Type | Article |
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Title | Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia |
Authors | Antia, I., Hills, F. and Shah, A. |
Abstract | Chondroitin sulphate (CS) and dermatan sulphate are linear negatively charged heteropolysaccharides. These glycosaminoglycans are involved in cellular signalling via binding to growth factors. CS is expressed in a range of tissue and biological fluids and is highly expressed in the placenta. There is evidence that decorin; a CS proteoglycan is significantly decreased in pre-eclampsia and fetal growth restriction. It is considered that GAG chain composition may influence cellular processes that are altered in pre-eclampsia. The goal of the present study was to develop an LC-MS method with precolumn procainamide labelling for the disaccharide compositional analysis of CS. The method was used to investigate whether the disaccharide composition of placenta-extracted CS is altered in pre-eclampsia. The study revealed differential disaccharide compositions of placental chondroitin sulphate disaccharide between pre-eclampsia and other pregnancy samples. This suggests that the method may have diagnostic potential for pregnancy disorders. Furthermore, the findings suggest that CS sulphation might play a significant role in maternal labour. |
Keywords | Chondroitin sulphate; dermatan sulphate; pregnancy disorders; LC-MS; procainamide |
Sustainable Development Goals | 3 Good health and well-being |
Middlesex University Theme | Health & Wellbeing |
Research Group | Reproductive Biology group |
Publisher | Royal Society of Chemistry (RSC) |
Journal | Analytical Methods |
ISSN | 1759-9660 |
Electronic | 1759-9679 |
Publication dates | |
Online | 08 Jan 2024 |
25 Jan 2024 | |
Publication process dates | |
Submitted | 06 Sep 2023 |
Accepted | 17 Dec 2023 |
Deposited | 11 Jan 2024 |
Output status | Published |
Accepted author manuscript | File Access Level Open |
Supplemental file | File Access Level Open |
Digital Object Identifier (DOI) | https://doi.org/10.1039/D3AY01578E |
PubMed ID | 38189556 |
Web of Science identifier | WOS:001142097600001 |
Language | English |
https://repository.mdx.ac.uk/item/xz75q
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