Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion

Conference poster

Hills, F., Rodd, Z. and Antia, I. 2018. Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion. International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e17-e17 https://doi.org/10.1016/j.placenta.2018.06.004
TypeConference poster
TitleEffect of glycosaminoglycans on growth factor-stimulated trophoblast invasion
AuthorsHills, F., Rodd, Z. and Antia, I.
Abstract

Objectives: To determine the effect of glycosaminoglycans and a series of growth factors on the viability and invasion of the extravillous trophoblast cell line SGHPL4.
Methods: Cells were cultured in Hams F10 media supplemented with fetal bovine serum and L-glutamine. For viability studies cells were seeded into 96-well culture plates (104 cells/well), maintained in serum free medium for 24h and then incubated with glycosaminoglycans (heparin, heparin sulphate and hyaluronic acid; each 100ng/ml) ± growth factors (VEGF, FGFand HB-EGF). Cell viability was measured in cells using the MTS assay. Cellular invasion was assessed using the FluoroBlok invasion assay. Cells were serum-starved for 24 h, incubated with the fluorescent dye DiIC12(3) (10mg/ml) for 1 hour prior to seeding onto an artificial extracellular matrix coated 8 mm FluoroBlok porous membrane inserts (2.5 x 105 cells per insert). Growth factors ± GAGs were added to the cell suspension and the inserts were lowered into a 96-well plate containing 10% fetal calf serum.
Plates were incubated at 37°C for 24h. Invasion was determined by measurement of fluorescence of invaded cells using a fluorescent plate reader (Ex549/Em565 nm).
Results: Cell numbers were significantly increased following incubation with VEGF, FGF and HB-EGF. Cell number was also increased after incubation with each of the glycosaminoglycans tested. The largest increase was observed following incubation with heparin sulphate. Cell numbers were further increased when the GFs were incubated with HS and heparin, but not with hyaluronic acid. Invasion was increased following incubation with VEGF, HBEGF and HGF. Heparan sulphate and heparin increased invasiveness in a dose-dependent manner. In contrast, hyaluronic acid had no significant effect.
Conclusion: This study demonstrates a role for glycosaminoglycans in key features of trophoblast function.

Research GroupReproductive Biology group
ConferenceInternational Federation of Placenta Associations Conference (IFPA2018)
Page rangee17-e17
ISSN0143-4004
PublisherElsevier
Publication dates
Print01 Sep 2018
Online10 Sep 2018
Publication process dates
Deposited18 Mar 2019
Accepted30 Jul 2018
Output statusPublished
Accepted author manuscript
License
Additional information

Poster abstract published in Placenta, Volume 69, September 2018, Pages e12-e93

Digital Object Identifier (DOI)https://doi.org/10.1016/j.placenta.2018.06.004
LanguageEnglish
Book titlePlacenta 69 (2018) e12e-e93
Permalink -

https://repository.mdx.ac.uk/item/8830z

Log in to edit

Download files

Accepted author manuscript
Oxford Abstracts2.pdf
License: CC BY-NC-ND 4.0

  • 69
    total views
  • 12
    total downloads
  • 3
    views this month
  • 0
    downloads this month

Export as

Related outputs

The efficacy of gemcitabine and docetaxel chemotherapy for the treatment of relapsed and refractory osteosarcoma: a systematic review and pre‐clinical study
Low, K., Foulkes, P., Hills, F., Roberts, H. and Stordal, B. 2024. The efficacy of gemcitabine and docetaxel chemotherapy for the treatment of relapsed and refractory osteosarcoma: a systematic review and pre‐clinical study. Cancer Medicine. 13 (18). https://doi.org/10.1002/cam4.70248
Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia
Antia, I., Hills, F. and Shah, A. 2024. Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia. Analytical Methods. 16 (4), pp. 566-575. https://doi.org/10.1039/D3AY01578E
Effect of hot water immersion on acute physiological responses following resistance exercise
Jackman, J., Bell, P., Van Someren, K., Gondek, M., Hills, F., Wilson, L. and Cockburn, E. 2023. Effect of hot water immersion on acute physiological responses following resistance exercise. Frontiers in Physiology. 14. https://doi.org/10.3389/fphys.2023.1213733
A survey into the use of priming techniques implemented by athletes and coaches to improve athletic performance
Collins, J., Bishop, C., Hills, F., Spiegelhalter, A., Cohen, R. and Turner, A. 2023. A survey into the use of priming techniques implemented by athletes and coaches to improve athletic performance. The Journal of Strength and Conditioning Research. 37 (1), pp. 107-113. https://doi.org/10.1519/jsc.0000000000004220
Establishment and characterization of single and triple‐agent resistant osteosarcoma cell lines
Low, K., Hills, F., Roberts, H. and Stordal, B. 2023. Establishment and characterization of single and triple‐agent resistant osteosarcoma cell lines. Advanced Biology. 7 (2). https://doi.org/10.1002/adbi.202200194
A combination of cherry juice and cold water immersion does not enhance marathon recovery compared to either treatment in isolation: a randomized placebo-controlled trial
Difranco, I., Cockburn, E., Dimitriou, L., Paice, K., Sinclair, S., Faki, T., Hills, F., Gondek, M., Wood, A. and Wilson, L. 2022. A combination of cherry juice and cold water immersion does not enhance marathon recovery compared to either treatment in isolation: a randomized placebo-controlled trial. Frontiers in Sports and Active Living. 4. https://doi.org/10.3389/fspor.2022.957950
ROR1-A novel biomarker for chemoresistance in ovarian cancer
Shafat, E., Hills, F. and Stordal, B. 2018. ROR1-A novel biomarker for chemoresistance in ovarian cancer. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
Optimisation of a selection strategy for drug resistance osteosarcoma cell lines
Low, K., Hills, F., Roberts, H. and Stordal, B. 2018. Optimisation of a selection strategy for drug resistance osteosarcoma cell lines. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
ROR1- A biomarker for chemoresistance in ovarian cancer
Syed, E., Hills, F. and Stordal, B. 2018. ROR1- A biomarker for chemoresistance in ovarian cancer. 2018 NCRI Cancer Conference. Glasgow, Scotland 04 - 06 Nov 2018
Optimisation of a selection strategy for drug resistance osteosarcoma cell lines
Low, K., Hills, F., Roberts, H. and Stordal, B. 2019. Optimisation of a selection strategy for drug resistance osteosarcoma cell lines. 2019 NCRI Cancer Conference. Glasgow, UK 03 - 05 Nov 2019
Cold water immersion offers no functional or perceptual benefit compared to a sham intervention during a resistance training program
Wilson, L., Dimitriou, L., Hills, F., Gondek, M., Van Wyk, A., Turek, V., Rivkin, T., Villiere, A., Jarvis, P., Miller, S., Turner, A. and Cockburn, E. 2021. Cold water immersion offers no functional or perceptual benefit compared to a sham intervention during a resistance training program. The Journal of Strength and Conditioning Research. 35 (10), pp. 2720-2727. https://doi.org/10.1519/jsc.0000000000004097
A pilot study to determine whether repeated post-exercise cryotherapy exposure augments physiological and perceptual responses to cold
Wilson, L., Dimitriou, L., Hills, F., Gondek, M., Van Wyk, A., Turek, V., Rivkin, T., Villiere, A., Jarvis, P., Miller, S., Turner, A. and Cockburn, E. 2020. A pilot study to determine whether repeated post-exercise cryotherapy exposure augments physiological and perceptual responses to cold. 25th Annual Congress of the European College of Sport Science. Virtual (due to Covid-19), initial venue Sevilla, Andalucia, Spain 28 - 30 Oct 2020
Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run
Dimitriou, L., Yagnik, D., Wood, A., Sinclair, S., Paice, K., Wilson, L., Hills, F., Gondek, M., Kwan Xue Bin, K., Howatson, G. and Cockburn, E. 2018. Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run. 23rd annual congress of the European College of Sport Science. Dublin, Ireland 04 - 07 Jul 2018
Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS
Gondek, M., Burczynska, B. and Hills, F. 2019. Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS. 4th FEBS Congress. Krakow, Poland 06 - 11 Jul 2019 Wiley. https://doi.org/10.1002/2211-5463.12674
The influence of repeated cold water immersion on adaptations to strength and power training
Wilson, L., Dimitriou, L., Gondek, M., Hills, F. and Cockburn, E. 2018. The influence of repeated cold water immersion on adaptations to strength and power training. 23rd Annual Congress of the ECSS 2018. Dublin, UK 04 - 07 Jul 2018 European College of Sport Science. pp. 94
Can quantification of Serum Glycans predict Pre-Eclampsia?
Antia, I., Shah, A., Yagnik, D., Syngelaki, A., Nicolaides, K. and Hills, F. 2018. Can quantification of Serum Glycans predict Pre-Eclampsia? International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e38-e39 https://doi.org/10.1016/j.placenta.2018.06.004
Whole body cryotherapy, cold water immersion, or a placebo following resistance exercise: a case of mind over matter?
Wilson, L., Dimitriou, L., Hills, F., Gondek, M. and Cockburn, E. 2019. Whole body cryotherapy, cold water immersion, or a placebo following resistance exercise: a case of mind over matter? European Journal of Applied Physiology. 119 (1), pp. 135-147. https://doi.org/10.1007/s00421-018-4008-7
Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone
Yagnik, D. and Hills, F. 2018. Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone. Molecular Medicine Reports. 18 (3), pp. 3506-3512. https://doi.org/10.3892/mmr.2018.9323
Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers
Gondek, M., Hills, F. and Burczynska, B. 2015. Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers. Summer Conference. Middlesex University, London, United Kingdom Jun 2015 Middlesex University.
Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS
Gondek, M., Burczynska, B. and Hills, F. 2018. Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS. 45th International Society of Oncology and BioMarkers (ISOBM) 2018 Congress. Hamburg, Germany 24 - 27 Nov 2018
Investigation of factors influencing the immunogenicity of hCG as a potential cancer vaccine
Kvirkvelia, N., Chikadze, N., Makinde, J., McBride, J., Porakishvili, N., Hills, F., Martensen, P., Justesen, J., Delves, P., Lund, T. and Roitt, I. 2018. Investigation of factors influencing the immunogenicity of hCG as a potential cancer vaccine. Clinical & Experimental Immunology. 193 (1), pp. 73-83. https://doi.org/10.1111/cei.13131
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, I., Mathew, K., Yagnik, D., Hills, F. and Shah, A. 2018. Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. Analytical and Bioanalytical Chemistry. 410 (1), pp. 131-143. https://doi.org/10.1007/s00216-017-0703-1
The effect of milk on recovery from repeat-sprint cycling in female team-sport athletes
Rankin, P., Lawlor, M., Hills, F., Bell, P., Stevenson, E. and Cockburn, E. 2018. The effect of milk on recovery from repeat-sprint cycling in female team-sport athletes. Applied Physiology, Nutrition, and Metabolism. 43 (2), pp. 113-122. https://doi.org/10.1139/apnm-2017-0275
Recovery following a marathon: a comparison of cold water immersion, whole body cryotherapy and a placebo control
Wilson, L., Cockburn, E., Paice, K., Sinclair, S., Faki, T., Hills, F., Gondek, M., Wood, A. and Dimitriou, L. 2018. Recovery following a marathon: a comparison of cold water immersion, whole body cryotherapy and a placebo control. European Journal of Applied Physiology. 118 (1), pp. 153-163. https://doi.org/10.1007/s00421-017-3757-z
Competition intensity and fatigue in elite fencing
Turner, A., Kilduff, L., Mrashall, G., Phillips, J., Noto, A., Buttigeig, C., Gondek, M., Hills, F. and Dimitriou, L. 2017. Competition intensity and fatigue in elite fencing. The Journal of Strength and Conditioning Research. 31 (11), pp. 3128-3136. https://doi.org/10.1519/JSC.0000000000001758
Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines
Gondek, M., Hills, F. and Burczynska, B. 2017. Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines. Summer Conference 2017. Middlesex University Jun 2017
The effect of plasma volume on changes in biomarkers of muscle damage, inflammation, oxidative stress and leukocyte differentialfollowing a 42.2 km trail run, and the efficacy of recovery interventions
Cockburn, E., Hills, F., Wilson, L., Paice, K., Wood, A. and Dimitriou, L. 2017. The effect of plasma volume on changes in biomarkers of muscle damage, inflammation, oxidative stress and leukocyte differentialfollowing a 42.2 km trail run, and the efficacy of recovery interventions. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s26
Influence of cold water immersion and cherry juice on recovery following a 42.2km trail run
Paice, K., Dimitriou, L., Wilson, L., Hills, F., Sinclair, S., Gondek, M., Kwan Xue Bin, X., Wood, A. and Cockburn, E. 2017. Influence of cold water immersion and cherry juice on recovery following a 42.2km trail run. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s92
The influence of cold water immersion on markers of recovery following resistance exercise
Wilson, L., Dimitriou, L., Hills, F., Gondek, M. and Cockburn, E. 2017. The influence of cold water immersion on markers of recovery following resistance exercise. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s66
Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring
Antia, I., Yagnik, D., Pantoja Munoz, L., Shah, A. and Hills, F. 2017. Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry. 530, pp. 17-30. https://doi.org/10.1016/j.ab.2017.04.019
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
Placental syndecan-1 and sulphated glycosaminoglycans are decreased in preeclampsia
Heyer-Chauhan, N., Ovbude, I., Hills, A., Sullivan, M. and Hills, F. 2013. Placental syndecan-1 and sulphated glycosaminoglycans are decreased in preeclampsia. Journal of Perinatal Medicine. https://doi.org/10.1515/jpm-2013-0097
IGFBP-1 in the placenta, membranes and fetal circulation: levels at term and preterm delivery
Hills, F., Gunn, L., Hardiman, P., Thanaratnam, S. and Chard, T. 1996. IGFBP-1 in the placenta, membranes and fetal circulation: levels at term and preterm delivery. Early Human Development. 44 (1), pp. 71-76.
Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy
Hills, F., Iles, R. and Sullivan, M. 2013. Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy. Journal of Perinatal Medicine. 41 (3), pp. 241-249. https://doi.org/10.1515/jpm-2012-0086
Insulin-like growth factor-II and heparin are anti-apoptotic survival factors in human villous cytotrophoblast
Hills, F., Mehmet, H. and Sullivan, M. 2012. Insulin-like growth factor-II and heparin are anti-apoptotic survival factors in human villous cytotrophoblast. European Journal of Obstetrics, Gynecology, and Reproductive Biology. 163 (1), pp. 11-16. https://doi.org/10.1016/j.ejogrb.2012.03.013
Superovulation, IGFBP-1 and birth weight.
Johnson, M., Irvine, R., Hills, F., Bolton, V., Abbas, A., Brooks, A., Allman, A., Chard, T. and Nicolaides, K. 1995. Superovulation, IGFBP-1 and birth weight. European Journal of Obstetrics, Gynaecology and Reproductive Biology. 59 (2), pp. 193-195.
The effects of labor on maternal and fetal levels of insulin-like growth factor binding protein-1
Hills, F., Crawford, R., Harding, S., Farkas, F. and Chard, T. 1994. The effects of labor on maternal and fetal levels of insulin-like growth factor binding protein-1. American Journal of Obstetrics and Gynecology. 171 (5), pp. 1292-1295.
Insulin-related growth factor binding protein-1 levels in ovum donation pregnancies.
Brooks, A., Johnson, M., Hills, F., Chard, T., Irvine, R. and Abdalla, H. 1995. Insulin-related growth factor binding protein-1 levels in ovum donation pregnancies. European Journal of Obstetrics, Gynaecology and Reproductive Biology. 59 (1), pp. 91-94.
Elevated levels of insulin-like growth factor binding protein-1 in fetal distress.
Crawford, R., Hills, F., Farkas, A. and Chard, T. 1995. Elevated levels of insulin-like growth factor binding protein-1 in fetal distress. British Journal of Obstetrics and Gynaecology. 102 (7), pp. 538-540.
Lack of evidence for a circadian rhythm of IGFBP-1 in the mother and fetus during labour.
Harding, S., Mensah, A., Hills, F., Howell, R. and Chard, T. 1995. Lack of evidence for a circadian rhythm of IGFBP-1 in the mother and fetus during labour. British Journal of Obstetrics and Gynaecology. 102 (11), pp. 891-893.
Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device.
Wolley, J., Saleem, S., Hills, F., Salem, H., El-Nashar, E. and Chard, T. 1996. Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. Gynecologic and Obstetric Investigation. 42 (4), pp. 241-243.
Circulating levels of IGF-I and IGF-binding protein-1 throughout pregnancy: relation to birthweight and maternal weight.
Hills, F., English, J. and Chard, T. 1996. Circulating levels of IGF-I and IGF-binding protein-1 throughout pregnancy: relation to birthweight and maternal weight. Journal of Endocrinology. 148 (2), pp. 303-309. https://doi.org/10.1677/joe.0.1480303
Antenatal dexamethasone therapy does not affect circulating concentrations of insulin-like growth factor binding protein-1.
Ogueh, O., Hills, F., Chard, T. and Johnson, M. 1998. Antenatal dexamethasone therapy does not affect circulating concentrations of insulin-like growth factor binding protein-1. Human Reproduction. 13 (6), pp. 1714-1716. https://doi.org/10.1093/humrep/13.6.1714
Effects of co-trimoxazole, erythromycin, amoxycillin, tetracycline and chloroquine on sperm function in vitro
Hargreaves, C., Rogers, S., Hills, F., Rahman, F., Howell, R. and Homa, S. 1998. Effects of co-trimoxazole, erythromycin, amoxycillin, tetracycline and chloroquine on sperm function in vitro. Human Reproduction. 13 (7), pp. 1878-1886.
Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression
Monk, D., Arnaud, P., Frost, J., Hills, F., Stanier, P., Feil, R. and Moore, G. 2009. Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression. Human Molecular Genetics. 18 (16), pp. 3066-3074. https://doi.org/10.1093/hmg/ddp248
Heparin prevents programmed cell death in human trophoblast
Hills, F., Abrahams, V., Gonzalez-Timon, B., Francis, J., Cloke, B., Hinkson, L., Rai, R., Regan, L., Mor, G., Sullivan, M., Lam, E. and Brosens, J. 2006. Heparin prevents programmed cell death in human trophoblast. Molecular Human Reproduction. 12 (4), pp. 237-242.
A longitudinal study of maternal plasma insulin-like growth factor binding protein-1 concentrations during normal pregnancy and pregnancies complicated by pre-eclampsia
Anim-Nyame, N., Hills, F., Soorana, S., Steer, P. and Johnson, M. 2000. A longitudinal study of maternal plasma insulin-like growth factor binding protein-1 concentrations during normal pregnancy and pregnancies complicated by pre-eclampsia. Human Reproduction. 15 (10), pp. 2215-2219. https://doi.org/10.1093/humrep/15.10.2215
Interactions between progesterone receptor isoforms in myometrial cells in human labour
Pieber, D., Allport, V., Hills, F., Johnson, M. and Bennett, P. 2001. Interactions between progesterone receptor isoforms in myometrial cells in human labour. Molecular Human Reproduction. 7 (9), pp. 875-879. https://doi.org/10.1093/molehr/7.9.875
Regulated expression of signal transducer and activator of transcription, Stat5, and its enhancement of PRL expression in human endometrial stromal cells in vitro
Mak, I., Brosens, J., Christian, M., Hills, F., Chamley, L., Regan, L. and White, J. 2002. Regulated expression of signal transducer and activator of transcription, Stat5, and its enhancement of PRL expression in human endometrial stromal cells in vitro. Journal of Clinical Endocrinology and Metabolism. 87 (6), pp. 2581-2588. https://doi.org/10.1210/jc.87.6.2581
Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells
Loudon, J., Elliott, C., Hills, F. and Bennett, P. 2003. Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells. Biology of Reproduction. 69 (1), pp. 331-337.
The relationship between insulin and insulin-like growth factor binding protein-1 is modified by pre-eclampsia.
Anim-Nyame, N., Hills, F., Soorana, S., Steer, P. and Johnson, M. 2003. The relationship between insulin and insulin-like growth factor binding protein-1 is modified by pre-eclampsia. Gynecological endocrinology.. 17 (6), pp. 471-476. https://doi.org/10.1080/09513590312331290408
The insulin-like growth factor system in mammalian pregnancy: a workshop report
Carter, A., Hills, F., O'Gorman, D., Robets, C., Soorana, S., Watson, C. and Westwood, M. 2004. The insulin-like growth factor system in mammalian pregnancy: a workshop report. Placenta. 25 (Supple), pp. S53-S56. https://doi.org/10.1016/j.placenta.2004.01.019
Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human
Monk, D., Sanches, R., Arnaud, P., Apostolidou, S., Hills, F., Abu-Amero, S., Murrell, A., Friess, H., Reik, W. and Stanier, P. 2006. Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human. Human Molecular Genetics. 15 (8), pp. 1259-1269. https://doi.org/10.1093/hmg/ddl041
STOX1 is not imprinted and is not likely to be involved in preeclampsia.
Hills, F., Iglesias-Platas, I., Jebbink, J. and Monk, D. 2007. STOX1 is not imprinted and is not likely to be involved in preeclampsia. Nature Genetics. 39 (3), pp. 279-280. https://doi.org/10.1038/ng0307-279
Limited evolutionary conservation of imprinting in the human placenta
Hills, F., Apostolidou, S., Arnaud, P. and Monk, D. 2006. Limited evolutionary conservation of imprinting in the human placenta. Proceedings of the National Academy of Sciences. 103 (17), pp. 6623-6628. https://doi.org/10.1073/pnas.0511031103
Regulation of human villous trophoblast by insulin-like growth factors and insulin-like growth factor-binding protein-1
Hills, F., Chard, T., Elder, M. and Sullivan, M. 2004. Regulation of human villous trophoblast by insulin-like growth factors and insulin-like growth factor-binding protein-1. Journal of Endocrinology. 183 (3), pp. 487-496. https://doi.org/10.1677/joe.1.05867
Polyclonal anti-tumor necrosis factor-alpha Fab used as an ancillary treatment for severe malaria
Looareesuwan, S., Sjorstrom, L., Krudsood, S., Wilairatana, P., Porter, R., Hills, F. and Warrell, D. 1999. Polyclonal anti-tumor necrosis factor-alpha Fab used as an ancillary treatment for severe malaria. The American Journal of Tropical Medicine and Hygiene. 61 (1), pp. 26-33. https://doi.org/10.4269/ajtmh.1999.61.26
Mechanism of action of the intrauterine contraceptive device: evidence for a specific biochemical deficiency in the endometrium
Seleem, S., Hills, F., Salem, H., El-Nashar, E. and Chard, T. 1996. Mechanism of action of the intrauterine contraceptive device: evidence for a specific biochemical deficiency in the endometrium. Human Reproduction. 11 (6), pp. 1220-1222.