Interactions between progesterone receptor isoforms in myometrial cells in human labour
Article
Pieber, D., Allport, V., Hills, F., Johnson, M. and Bennett, P. 2001. Interactions between progesterone receptor isoforms in myometrial cells in human labour. Molecular Human Reproduction. 7 (9), pp. 875-879. https://doi.org/10.1093/molehr/7.9.875
Type | Article |
---|---|
Title | Interactions between progesterone receptor isoforms in myometrial cells in human labour |
Authors | Pieber, D., Allport, V., Hills, F., Johnson, M. and Bennett, P. |
Abstract | Progesterone acts to maintain uterine quiescence during pregnancy. In contrast to many other species, no decrease in maternal serum levels of progesterone can be observed in humans before the onset of labour. Therefore, a `functional' progesterone withdrawal in association with labour has been proposed. In humans the progesterone receptor (PR) exists in two isoforms, PR-A and PR-B. While PR-B generally mediates the effects of progesterone upon gene transcription, the role of PR-A during pregnancy, and in parturition, is unknown. In this study, term myometrium cells cultured before the onset of labour were transiently transfected with expression vectors for either PR-A or PR-B. Only those cells expressing PR-B significantly increased expression of a progesterone-sensitive reporter when stimulated with progesterone. Co-transfection of both isoforms of PR demonstrated that PR-A is a dominant repressor of transactivation in these cells. Western blot analysis showed that PR-A is present in human myometrium samples taken only after, but not before, the onset of labour. These data suggest that increased expression of PR-A in human myometrium may contribute to `functional' progesterone withdrawal and the initiation of human labour. |
Research Group | Centre for Investigative and Diagnostic Oncology |
Biomarkers for Cancer group | |
Reproductive Biology group | |
Publisher | Oxford University Press (OUP) |
Journal | Molecular Human Reproduction |
ISSN | 1460-2407 |
Publication dates | |
Sep 2001 | |
Publication process dates | |
Deposited | 17 Jun 2009 |
Output status | Published |
Additional information | PubMed PMID: 11517295. |
Digital Object Identifier (DOI) | https://doi.org/10.1093/molehr/7.9.875 |
Language | English |
https://repository.mdx.ac.uk/item/81qyw
66
total views0
total downloads0
views this month0
downloads this month