Optimisation of a selection strategy for drug resistance osteosarcoma cell lines

Conference item

Low, K., Hills, F., Roberts, H. and Stordal, B. 2018. Optimisation of a selection strategy for drug resistance osteosarcoma cell lines. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
TitleOptimisation of a selection strategy for drug resistance osteosarcoma cell lines
AuthorsLow, K., Hills, F., Roberts, H. and Stordal, B.
Abstract

Osteosarcoma is the most common primary malignant bone tumour and also the second highest cause of cancer related death in children and teenagers. The treatment of osteosarcoma including the use of neo-adjuvant chemotherapy in combination with surgery has increased the long-term survival rate of patients in last few decades. The most effective anticancer drug used against osteosarcoma are cisplatin, doxorubicin and high-dose methotrexate. The cure rate of osteosarcoma by surgery ranges from 15% to 20% and improves dramatically to 70% in the combination with chemotherapy. However, additional chemotherapy is palliative and toxic to approximately 40% of the patients with progression of osteosarcoma after front-line therapy. The aim of this study is to establish new drug-resistant osteosarcoma cell lines and investigate their mechanism of resistance. The human osteosarcoma cell lines MG-63 and HOS-143b will be treated with cisplatin, methotrexate and doxorubicin. The cell lines will expose to clinically relevant doses in the medium to select for the drug-resistant sublines. The morphological features of the drug-resistant subline will be observed using inverted microscopy. The growth rate of the developing drug-resistant sublines of MG-63 and HOS-143b will be calculated using an Image J method for determining cell confluence. The IC50s of each drug will be established in each cell line to optimise the doses used to treat the parental cell lines. The medium condition of MG-63 and HOS-143b is being optimised to mimic the clinical situation by removing antibiotic for both cell lines and bromodeoxyuridine (BrDU) for HOS143b.The growth rate of MG-63 and HOS-143b has been calculated and the doubling time is 34.15 ± 7.707 and 24.6 hours respectively. The Image J method for cell confluence has been optimised and shows a strong correlation to manual cell counts for each cell line. The correlation of Area Fraction and cell count for MG-63 is r=0.9823, which is significant with p value = 0.0005. For HOS-143b, the correlation is r=0.9823, which is also significant with p value = 0.0234. The expected outcome of this study is the establishment of drug-resistant sublines from MG-63 and HOS-143b. The drug-resistant sublines from MG-63 and HOS-143b will obtain a higher fold of resistant to their respective drugs in comparison with their parental cell lines and may also exhibit crossresistance to other drugs. These resistant sublines will be invaluable tools with which to study the resistance of anticancer drugs and to identify the methods to overcome resistance.

Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
Research GroupBiomarkers for Cancer group
ConferenceResearch Degrees Students' Summer Conference
Publication dates
Print04 Sep 2018
Publication process dates
Deposited25 Jul 2022
Accepted04 Aug 2018
Output statusPublished
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/89xyz

Log in to edit
  • 60
    total views
  • 0
    total downloads
  • 4
    views this month
  • 0
    downloads this month

Export as

Related outputs

Breast cancer risk and prevention in 2024: An overview from the Breast Cancer UK ‐ Breast Cancer Prevention Conference
Stordal, B., Harvie, M., Antoniou, M.N., Bellingham, M., Chan, D.S.M., Darbre, P., Karlsson, O., Kortenkamp, A., Magee, P., Mandriota, S., Silva, E., Turner, J.E., Vandenberg, L.N. and Evans, D.G. 2024. Breast cancer risk and prevention in 2024: An overview from the Breast Cancer UK ‐ Breast Cancer Prevention Conference. Cancer Medicine. 13 (18). https://doi.org/10.1002/cam4.70255
The efficacy of gemcitabine and docetaxel chemotherapy for the treatment of relapsed and refractory osteosarcoma: a systematic review and pre‐clinical study
Low, K., Foulkes, P., Hills, F., Roberts, H. and Stordal, B. 2024. The efficacy of gemcitabine and docetaxel chemotherapy for the treatment of relapsed and refractory osteosarcoma: a systematic review and pre‐clinical study. Cancer Medicine. 13 (18). https://doi.org/10.1002/cam4.70248
Chromosomal copy number and mutational status are required to authenticate ovarian cancer cell lines as appropriate cell models
Stordal, B., Farrelly, A. and Hennessy, B. 2024. Chromosomal copy number and mutational status are required to authenticate ovarian cancer cell lines as appropriate cell models. Molecular Biology Reports. 51 (1). https://doi.org/10.1007/s11033-024-09747-4
Ovarian cancer: identifying and managing familial and genetic risk
Slade, E., Berg, L., Dworzynski, K., Manchanda, R., Barber, V., Bowen, R., Brentnall, A., Cameron, A., Davies, D., Davies, M., Evans, D., Hayward, J., McCluggage, W., Miles, T., Monahan, K., Moses, D., Robb, F., Rosenthal, A., Side, L., Stanford, J., Stordal, B. and Tripathi, V. 2024. Ovarian cancer: identifying and managing familial and genetic risk. National Institute for Health and Care Excellence.
Ovarian cancer: identifying and managing familial and genetic risk—summary of new NICE guidance
Slade, E., Berg, L., Dworzynski, K., Manchanda, R., Barber, V., Bowen, R., Brentnall, A., Cameron, A., Davies, D., Davies, M., Evans, D., Hayward, J., McCluggage, W., Miles, T., Monahan, K., Moses, D., Robb, F., Rosenthal, A., Side, L., Stanford, J., Stordal, B. and Tripathi, V. 2024. Ovarian cancer: identifying and managing familial and genetic risk—summary of new NICE guidance. British Medical Journal. 385. https://doi.org/10.1136/bmj.q807
Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia
Antia, I., Hills, F. and Shah, A. 2024. Disaccharide compositional analysis of chondroitin sulphate using WAX HILIC-MS with pre-column procainamide labelling; application to the placenta in pre-eclampsia. Analytical Methods. 16 (4), pp. 566-575. https://doi.org/10.1039/D3AY01578E
Identifying fibroblast growth factor receptor 3 as a mediator of periosteal osteochondral differentiation through the construction of microRNA-based interaction networks
Wells, L., Roberts, H., Luyten, F. and Roberts, S. 2023. Identifying fibroblast growth factor receptor 3 as a mediator of periosteal osteochondral differentiation through the construction of microRNA-based interaction networks. Biology. 12 (11). https://doi.org/10.3390/biology12111381
Effect of hot water immersion on acute physiological responses following resistance exercise
Jackman, J., Bell, P., Van Someren, K., Gondek, M., Hills, F., Wilson, L. and Cockburn, E. 2023. Effect of hot water immersion on acute physiological responses following resistance exercise. Frontiers in Physiology. 14. https://doi.org/10.3389/fphys.2023.1213733
A machine learning‑based image segmentation method to quantify in vitro osteoclast culture endpoints
Davies, B.K., Hibbert, A.P., Roberts, S.J., Roberts, H., Tickner, J.C., Holdsworth, G., Arnett, T.R. and Orriss, I.R. 2023. A machine learning‑based image segmentation method to quantify in vitro osteoclast culture endpoints. Calcified Tissue International and Musculoskeletal Research. 113 (4), p. 437–448.
Awareness of ovarian cancer symptoms and risk factors in a young ethnically diverse British population
Radu, C., Matos de Melo Fernandes, N., Khalfe, S. and Stordal, B. 2023. Awareness of ovarian cancer symptoms and risk factors in a young ethnically diverse British population. Cancer Medicine. 12 (8), pp. 9879-9892. https://doi.org/10.1002/cam4.5670
A survey into the use of priming techniques implemented by athletes and coaches to improve athletic performance
Collins, J., Bishop, C., Hills, F., Spiegelhalter, A., Cohen, R. and Turner, A. 2023. A survey into the use of priming techniques implemented by athletes and coaches to improve athletic performance. The Journal of Strength and Conditioning Research. 37 (1), pp. 107-113. https://doi.org/10.1519/jsc.0000000000004220
Establishment and characterization of single and triple‐agent resistant osteosarcoma cell lines
Low, K., Hills, F., Roberts, H. and Stordal, B. 2023. Establishment and characterization of single and triple‐agent resistant osteosarcoma cell lines. Advanced Biology. 7 (2). https://doi.org/10.1002/adbi.202200194
Breastfeeding reduces the risk of breast cancer: a call for action in high-income countries with low rates of breastfeeding
Stordal, B. 2023. Breastfeeding reduces the risk of breast cancer: a call for action in high-income countries with low rates of breastfeeding. Cancer Medicine. 12 (4), pp. 4616-4625. https://doi.org/10.1002/cam4.5288
Biomedical Science Exchanges at Middlesex University London
Stordal, B. 2019. Biomedical Science Exchanges at Middlesex University London. REALISE: Seminar on International Staff Mobility. Universities UK Boardroom, Woburn House, WC1H 9HQ, London
Biomedical Science Exchanges
Stordal, B. 2018. Biomedical Science Exchanges. Middlesex University’s Erasmus Staff Training Week 2018. Middlesex University London
Biomedical Science Exchanges
Stordal, B. 2018. Biomedical Science Exchanges. Middlesex University Staff Conference 2018: Learning through Change. Middlesex University London 27 Mar 2018
A combination of cherry juice and cold water immersion does not enhance marathon recovery compared to either treatment in isolation: a randomized placebo-controlled trial
Difranco, I., Cockburn, E., Dimitriou, L., Paice, K., Sinclair, S., Faki, T., Hills, F., Gondek, M., Wood, A. and Wilson, L. 2022. A combination of cherry juice and cold water immersion does not enhance marathon recovery compared to either treatment in isolation: a randomized placebo-controlled trial. Frontiers in Sports and Active Living. 4. https://doi.org/10.3389/fspor.2022.957950
Comparison of formative multiple choice questions using online platforms vs summative assessment: experience from an anatomy and physiology module
Stordal, B. 2015. Comparison of formative multiple choice questions using online platforms vs summative assessment: experience from an anatomy and physiology module. Middlesex University Annual Learning and Teaching Conference 2015: Revisiting Assessment. London, UK 13 Jul 2015
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
ROR1-A novel biomarker for chemoresistance in ovarian cancer
Shafat, E., Hills, F. and Stordal, B. 2018. ROR1-A novel biomarker for chemoresistance in ovarian cancer. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
Mechanisms of apoptosis and autophagy in chemoresistant ovarian cancer cells
Bennacer, A., Roberts, H. and Stordal, B. 2018. Mechanisms of apoptosis and autophagy in chemoresistant ovarian cancer cells. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
Tumour profiling tests to guide adjuvant chemotherapy decisions in early breast cancer: Diagnostics guidance [DG34]
Nixon, F., Albrow, R., Donna, B., Kroese, D., Adair, E., Bagshaw, J., Carrol, E., Driskell, O., Edwards, S., Fleming, S., Gray, J., Halligan, S., Hitchman, J., Hyde, C., McGinley, P., Messenger, M., Moseley, A., Naylor, P., Neely, D., Rahman Haley, S., Richards, S., Sculpher, M., Stevenson, M., Summerton, N., Wierzbicki, A., Bramley, M., Graham, J., Pepper, L., Ryyan, D., Stordal, B., Van Mann, U. and Wardley, A. 2018. Tumour profiling tests to guide adjuvant chemotherapy decisions in early breast cancer: Diagnostics guidance [DG34]. NICE.
Cross resistance between cisplatin and oxaliplatin in a low-level resistance lung cancer cell model
Stordal, B., Davey, M. and Davey, R. 2005. Cross resistance between cisplatin and oxaliplatin in a low-level resistance lung cancer cell model. 96th AACR Annual Meeting. Anaheim California 16 - 20 Apr 2005 American Association for Cancer Research. pp. 348-349
Oxaliplatin and cisplatin cause similar chromosomal changes in H69 SCLC cells: linking changes in genotype to the resistant phenotype
Stordal, B., Peters, G., St. Heaps, L. and Davey, R. 2006. Oxaliplatin and cisplatin cause similar chromosomal changes in H69 SCLC cells: linking changes in genotype to the resistant phenotype. 97th AACR Annual Meeting. Washington D.C., USA 01 - 05 Apr 2006 American Association for Cancer Research. pp. 133
BRCA1/2 mutation analysis in 43 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation
Stordal, B., Timms, K., Renuad, M., Thery, J., Farrelly, A., Williams, D., Potter, J., Tran, T., Korpanty, G., Cremona, M., Carey, M., Li, J., Li, Y., Aslan, O., O'Leary, J., Mills, G. and Hennessy, B. 2012. BRCA1/2 mutation analysis in 43 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation. 103rd Annual Meeting of the American Association for Cancer Research (AACR). Chicago 31 Mar - 04 Apr 2012 American Association for Cancer Research. https://doi.org/10.1158/1538-7445.AM2012-78
A systematic approach to the study of cancer stem cells applied to ovarian cancer
Ffrench, B., Cooke, A., Stordal, B., O'Toole, S., Sheils, O., Gallagher, M. and O'Leary, J. 2012. A systematic approach to the study of cancer stem cells applied to ovarian cancer. 103rd Annual Meeting of the American Association for Cancer Research (ACCR). Chicago, USA 31 Mar - 04 Apr 2012 American Association for Cancer Research. https://doi.org/10.1158/1538-7445.AM2012-3382
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. 2018 NCRI Cancer Conference. Glasgow, Scotland 04 - 06 Nov 2018
ROR1- A biomarker for chemoresistance in ovarian cancer
Syed, E., Hills, F. and Stordal, B. 2018. ROR1- A biomarker for chemoresistance in ovarian cancer. 2018 NCRI Cancer Conference. Glasgow, Scotland 04 - 06 Nov 2018
Optimisation of a selection strategy for drug resistance osteosarcoma cell lines
Low, K., Hills, F., Roberts, H. and Stordal, B. 2019. Optimisation of a selection strategy for drug resistance osteosarcoma cell lines. 2019 NCRI Cancer Conference. Glasgow, UK 03 - 05 Nov 2019
The EMT­activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but predictive of survival
Rae, S., Keenan, J., Spillane, C., Blackshields, G., Madden, S. and Stordal, B. 2015. The EMT­activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but predictive of survival. 2015 NCRI Cancer Conference. Liverpool, UK 01 - 03 Nov 2015
BRCA1 promoter methylation and clinical outcomes in epithelial ovarian cancer a pooled analysis of individual patient data
Kalachand, R., Stordal, B., Madden, S., Chandler, B., Cunningham, J., Goode, E., Ruscito, I., Braicu, E., Sehouli, J., Ignatov, A., Yu, H., Katsaros, D., Lu, L., Mills, G., Broaddus, R., Lu, K., Carey, M., Timms, K., Rzepecka, I., Kupryjanczyk, J., Swisher, E., Harrell, M., Agnew, K., O'Riain, C., O'Toole, S., O'Leary, J., Thomas, D., Chaudhry, P., Srinivasan, R. and Hennessy, B. 2019. BRCA1 promoter methylation and clinical outcomes in epithelial ovarian cancer a pooled analysis of individual patient data. Lorne Cancer Conference. Lorne, Victoria, Australia 14 - 16 Feb 2019
Comparative selection strategies for development of platinum-drug resistant ovarian cancer cell lines
Busschots, S., Kalachand, R., O'Toole, S., O'Leary, J., Hennessy, B. and Stordal, B. 2017. Comparative selection strategies for development of platinum-drug resistant ovarian cancer cell lines. 12th International Symposium on Platinum Coordination Compounds in Cancer Chemotherapy. Sydney, Australia 10 - 14 Dec 2017
PO-495 PI3K pathway upregulation mediates acquired resistance to platinum agents and polyadenoribose polymerase inhibitors (PARPi) in BRCA1-methylated ovarian cancer (OC)
Kalachand, R., Cremona, M., Farrelly, A., Eustace, A., Stordal, B. and Hennessy, B. 2018. PO-495 PI3K pathway upregulation mediates acquired resistance to platinum agents and polyadenoribose polymerase inhibitors (PARPi) in BRCA1-methylated ovarian cancer (OC). EACR25: The 25th Biennial Congress of the European Association for Cancer Research. Amsterdam, Netherlands 30 Jun - 03 Jul 2018
The role of autophagy in canine osteosarcoma chemoresistance and metastasis
Kulatheepan, Y., Baines, S., Meeson, R. and Roberts, H. 2022. The role of autophagy in canine osteosarcoma chemoresistance and metastasis. 26th Annual Technological Advances in Science, Medicine and Engineering Conference 2022. Virtual 27 - 28 Aug 2022
ATR inhibition increases sensitivity of a highly aggressive osteosarcoma cell line to cisplatin treatment
Pereira, J., Roberts, S. and Roberts, H. 2022. ATR inhibition increases sensitivity of a highly aggressive osteosarcoma cell line to cisplatin treatment. Research Students' Summer Conference. Middlesex University, Lonon, UK 22 - 23 Jun 2022
Migratory bodies express markers of tumour initiating cells and may represent an early stage of osteosarcoma sarcosphere initiation and metastasis
Paternina Martinez, D., Roberts, S. and Roberts, H. 2022. Migratory bodies express markers of tumour initiating cells and may represent an early stage of osteosarcoma sarcosphere initiation and metastasis. Research Students' Summer Conference. Middlesex University, Lonon, UK 22 - 23 Jun 2022
CRISPR/Cas9-mediated ATG7 knockout results in enhanced osteosarcoma chemosensitivity
Pears, A. and Roberts, H. 2022. CRISPR/Cas9-mediated ATG7 knockout results in enhanced osteosarcoma chemosensitivity. Bone Research Society Annual Conference. Manchester Metropolitan University, Manchester, UK 06 - 08 Jul 2022
ATR inhibition increases sensitivity of a highly aggressive osteosarcoma cell line to cisplatin treatment
Pereira, J., Roberts, S. and Roberts, H. 2022. ATR inhibition increases sensitivity of a highly aggressive osteosarcoma cell line to cisplatin treatment. Bone Research Society Annual Conference. Manchester Metropolitan University, Manchester, UK 06 - 08 Jul 2022
Migratory bodies express markers of tumour initiating cells and may represent an early stage of osteosarcoma sarcosphere initiation and metastasis
Paternina Martinez, D., Roberts, S. and Roberts, H. 2022. Migratory bodies express markers of tumour initiating cells and may represent an early stage of osteosarcoma sarcosphere initiation and metastasis. Bone Research Society Annual Conference. Manchester Metropolitan University, Manchester, UK 06 - 08 Jul 2022
Intentionality for inclusivity - the journey at Middlesex University
Roberts, H., Punev, I., Allardyce, D., Kyprianou, A., Appiah, S., Megeney, A., Calin, A., Gallacher, D. and Mill, R. 2022. Intentionality for inclusivity - the journey at Middlesex University. McGraw Hill.
Taxane monotherapy regimens for the treatment of recurrent epithelial ovarian cancer [Review - Intervention]
Patel, A., Kalachand, R., Busschots, S., Doherty, B., Kapros, E., Lawlor, D., Hall, N. and Stordal, B. 2022. Taxane monotherapy regimens for the treatment of recurrent epithelial ovarian cancer [Review - Intervention]. Cochrane Database of Systematic Reviews. https://doi.org/10.1002/14651858.CD008766.pub3
The EMT-activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but is predictive of survival
Rae, S., Spillane, C., Blackshields, G., Madden, S., Keenan, J. and Stordal, B. 2022. The EMT-activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but is predictive of survival. Human Cell. 35 (5), pp. 1547-1559. https://doi.org/10.1007/s13577-022-00744-y
Decellularised cartilage ECM culture coatings drive rapid and robust chondrogenic differentiation of human periosteal cells
Vas, W., Shah, M., Roberts, H. and Roberts, S. 2022. Decellularised cartilage ECM culture coatings drive rapid and robust chondrogenic differentiation of human periosteal cells. Bioengineering. 9 (5). https://doi.org/10.3390/bioengineering9050203
Cold water immersion offers no functional or perceptual benefit compared to a sham intervention during a resistance training program
Wilson, L., Dimitriou, L., Hills, F., Gondek, M., Van Wyk, A., Turek, V., Rivkin, T., Villiere, A., Jarvis, P., Miller, S., Turner, A. and Cockburn, E. 2021. Cold water immersion offers no functional or perceptual benefit compared to a sham intervention during a resistance training program. The Journal of Strength and Conditioning Research. 35 (10), pp. 2720-2727. https://doi.org/10.1519/jsc.0000000000004097
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
Klionsky, D., Roberts, H. and et al. 2021. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy. 17 (1), pp. 1-382. https://doi.org/10.1080/15548627.2020.1797280
A pilot study to determine whether repeated post-exercise cryotherapy exposure augments physiological and perceptual responses to cold
Wilson, L., Dimitriou, L., Hills, F., Gondek, M., Van Wyk, A., Turek, V., Rivkin, T., Villiere, A., Jarvis, P., Miller, S., Turner, A. and Cockburn, E. 2020. A pilot study to determine whether repeated post-exercise cryotherapy exposure augments physiological and perceptual responses to cold. 25th Annual Congress of the European College of Sport Science. Virtual (due to Covid-19), initial venue Sevilla, Andalucia, Spain 28 - 30 Oct 2020
Prevalence of tumor BRCA1 and BRCA2 dysfunction in unselected patients with ovarian cancer
Kalachand, R., O'Riain, C., Toomey, S., Carr, A., Timms, K., O'Toole, S., Madden, S., Bates, M., O'Leary, J., Gleeson, N., O'Donnell, D., Grogan, L., Breathnach, O., Farrelly, A., Stordal, B. and Hennessy, B. 2020. Prevalence of tumor BRCA1 and BRCA2 dysfunction in unselected patients with ovarian cancer. Obstetrics & Gynecology Science. 63 (5), pp. 643-654. https://doi.org/10.5468/ogs.20033
BRCA1 promoter methylation and clinical outcomes in ovarian cancer: an individual patient data meta-analysis
Kalachand, R., Stordal, B., Madden, S., Chandler, B., Cunningham, J., Goode, E., Ruscito, I., Braicu, E., Sehouli, J., Ignatov, A., Yu, H., Katsaros, D., Mills, G., Lu, K., Carey, M., Timms, K., Kupryjanczyk, J., Rzepecka, I., Podgorska, A., McAlpine, J., Swisher, E., Bernards, S., O'Riain, C., O'Toole, S., O'Leary, J., Bowtell, D., Thomas, D., Prieske, K., Joosse, S., Woelber, L., Chaudhry, P., Häfner, N., Runnebaum, I. and Hennessy, B. 2020. BRCA1 promoter methylation and clinical outcomes in ovarian cancer: an individual patient data meta-analysis. Journal of the National Cancer Institute. 112 (12), pp. 1190-1203. https://doi.org/10.1093/jnci/djaa070
Mapping human serum induced gene networks as a basis for the creation of biomimetic periosteum for bone repair
Al Hosni, R., Shah, M., Cheema, U., Roberts, H., Luyten, F. and Roberts, S. 2020. Mapping human serum induced gene networks as a basis for the creation of biomimetic periosteum for bone repair. Cytotherapy. 22 (8), pp. 424-435.
Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run
Dimitriou, L., Yagnik, D., Wood, A., Sinclair, S., Paice, K., Wilson, L., Hills, F., Gondek, M., Kwan Xue Bin, K., Howatson, G. and Cockburn, E. 2018. Influence of tart cherry juice on airway biomarkers of inflammation and oxidative stress following a 42.2km trail run. 23rd annual congress of the European College of Sport Science. Dublin, Ireland 04 - 07 Jul 2018
Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS
Gondek, M., Burczynska, B. and Hills, F. 2019. Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS. 4th FEBS Congress. Krakow, Poland 06 - 11 Jul 2019 Wiley. https://doi.org/10.1002/2211-5463.12674
The influence of repeated cold water immersion on adaptations to strength and power training
Wilson, L., Dimitriou, L., Gondek, M., Hills, F. and Cockburn, E. 2018. The influence of repeated cold water immersion on adaptations to strength and power training. 23rd Annual Congress of the ECSS 2018. Dublin, UK 04 - 07 Jul 2018 European College of Sport Science. pp. 94
Can quantification of Serum Glycans predict Pre-Eclampsia?
Antia, I., Shah, A., Yagnik, D., Syngelaki, A., Nicolaides, K. and Hills, F. 2018. Can quantification of Serum Glycans predict Pre-Eclampsia? International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e38-e39 https://doi.org/10.1016/j.placenta.2018.06.004
Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion
Hills, F., Rodd, Z. and Antia, I. 2018. Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion. International Federation of Placenta Associations Conference (IFPA2018). Jikei University School of Medicine in Tokyo, Japan 21 - 24 Sep 2018 Elsevier. pp. e17-e17 https://doi.org/10.1016/j.placenta.2018.06.004
The combination of curcumin and doxorubicin shows a selective cytotoxic effect on acute myeloid leukaemia cell line
Hasan, N., Roberts, H., Ghali, L. and Appiah, S. 2018. The combination of curcumin and doxorubicin shows a selective cytotoxic effect on acute myeloid leukaemia cell line. NCRI Cancer Conference (2018). Glasgow, Scotland 04 - 06 Nov 2018
Whole body cryotherapy, cold water immersion, or a placebo following resistance exercise: a case of mind over matter?
Wilson, L., Dimitriou, L., Hills, F., Gondek, M. and Cockburn, E. 2019. Whole body cryotherapy, cold water immersion, or a placebo following resistance exercise: a case of mind over matter? European Journal of Applied Physiology. 119 (1), pp. 135-147. https://doi.org/10.1007/s00421-018-4008-7
MicroRNA profiles are associated with lymphocyte count after a major traumatic injury: a potential role for immunometabolism?
Jones, T., Torrance, H., Roberts, H., Brohi, K., Barnes, M., Knight, J., Hinds, C., Ackland, G. and O'Dwyer, M. 2018. MicroRNA profiles are associated with lymphocyte count after a major traumatic injury: a potential role for immunometabolism? British Journal of Anaesthesia Research Forum. Royal College of Anaesthetists, London 30 - 31 May 2018 Elsevier. pp. e23-e24 https://doi.org/10.1016/j.bja.2018.05.027
Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone
Yagnik, D. and Hills, F. 2018. Urate crystals induce macrophage PAF‑AH secretion which is differentially regulated by TGFβ1 and hydrocortisone. Molecular Medicine Reports. 18 (3), pp. 3506-3512. https://doi.org/10.3892/mmr.2018.9323
Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers
Gondek, M., Hills, F. and Burczynska, B. 2015. Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers. Summer Conference. Middlesex University, London, United Kingdom Jun 2015 Middlesex University.
Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS
Gondek, M., Burczynska, B. and Hills, F. 2018. Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS. 45th International Society of Oncology and BioMarkers (ISOBM) 2018 Congress. Hamburg, Germany 24 - 27 Nov 2018
Taxane monotherapy regimens for the treatment of recurrent epithelial ovarian cancer [Protocol - Intervention]
Stordal, B., Kalachand, R. and Hall, N. 2018. Taxane monotherapy regimens for the treatment of recurrent epithelial ovarian cancer [Protocol - Intervention]. Cochrane Database of Systematic Reviews. https://doi.org/10.1002/14651858.cd008766.pub2
Investigation of factors influencing the immunogenicity of hCG as a potential cancer vaccine
Kvirkvelia, N., Chikadze, N., Makinde, J., McBride, J., Porakishvili, N., Hills, F., Martensen, P., Justesen, J., Delves, P., Lund, T. and Roitt, I. 2018. Investigation of factors influencing the immunogenicity of hCG as a potential cancer vaccine. Clinical & Experimental Immunology. 193 (1), pp. 73-83. https://doi.org/10.1111/cei.13131
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, I., Mathew, K., Yagnik, D., Hills, F. and Shah, A. 2018. Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. Analytical and Bioanalytical Chemistry. 410 (1), pp. 131-143. https://doi.org/10.1007/s00216-017-0703-1
The effect of milk on recovery from repeat-sprint cycling in female team-sport athletes
Rankin, P., Lawlor, M., Hills, F., Bell, P., Stevenson, E. and Cockburn, E. 2018. The effect of milk on recovery from repeat-sprint cycling in female team-sport athletes. Applied Physiology, Nutrition, and Metabolism. 43 (2), pp. 113-122. https://doi.org/10.1139/apnm-2017-0275
Recovery following a marathon: a comparison of cold water immersion, whole body cryotherapy and a placebo control
Wilson, L., Cockburn, E., Paice, K., Sinclair, S., Faki, T., Hills, F., Gondek, M., Wood, A. and Dimitriou, L. 2018. Recovery following a marathon: a comparison of cold water immersion, whole body cryotherapy and a placebo control. European Journal of Applied Physiology. 118 (1), pp. 153-163. https://doi.org/10.1007/s00421-017-3757-z
Competition intensity and fatigue in elite fencing
Turner, A., Kilduff, L., Mrashall, G., Phillips, J., Noto, A., Buttigeig, C., Gondek, M., Hills, F. and Dimitriou, L. 2017. Competition intensity and fatigue in elite fencing. The Journal of Strength and Conditioning Research. 31 (11), pp. 3128-3136. https://doi.org/10.1519/JSC.0000000000001758
CD10−/ALDH− cells are the sole cisplatin-resistant component of a novel ovarian cancer stem cell hierarchy
Ffrench, B., Gasch, C., Hokamp, K., Spillane, C., Blackshields, G., Mahgoub, T., Bates, M., Kehoe, L., Mooney, A., Doyle, R., Doyle, B., O'Donnell, D., Gleeson, N., Hennessy, B., Stordal, B., O'Riain, C., Lambkin, H., O'Toole, S., O'Leary, J. and Gallagher, M. 2017. CD10−/ALDH− cells are the sole cisplatin-resistant component of a novel ovarian cancer stem cell hierarchy. Cell Death & Disease. 8 (10). https://doi.org/10.1038/cddis.2017.379
Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines
Gondek, M., Hills, F. and Burczynska, B. 2017. Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines. Summer Conference 2017. Middlesex University Jun 2017
The effect of plasma volume on changes in biomarkers of muscle damage, inflammation, oxidative stress and leukocyte differentialfollowing a 42.2 km trail run, and the efficacy of recovery interventions
Cockburn, E., Hills, F., Wilson, L., Paice, K., Wood, A. and Dimitriou, L. 2017. The effect of plasma volume on changes in biomarkers of muscle damage, inflammation, oxidative stress and leukocyte differentialfollowing a 42.2 km trail run, and the efficacy of recovery interventions. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s26
Influence of cold water immersion and cherry juice on recovery following a 42.2km trail run
Paice, K., Dimitriou, L., Wilson, L., Hills, F., Sinclair, S., Gondek, M., Kwan Xue Bin, X., Wood, A. and Cockburn, E. 2017. Influence of cold water immersion and cherry juice on recovery following a 42.2km trail run. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s92
The influence of cold water immersion on markers of recovery following resistance exercise
Wilson, L., Dimitriou, L., Hills, F., Gondek, M. and Cockburn, E. 2017. The influence of cold water immersion on markers of recovery following resistance exercise. BASES-FEPSAC Conference 2017. Nottingham, UK 28 - 29 Nov 2017 Taylor & Francis (Routledge). pp. s66
Phytochemical modulation of apoptosis and autophagy: strategies to overcome chemoresistance in leukaemic stem cells in the bone marrow microenvironment
Owen, H., Appiah, S., Hasan, N., Ghali, L., Elayat, G. and Bell, C. 2017. Phytochemical modulation of apoptosis and autophagy: strategies to overcome chemoresistance in leukaemic stem cells in the bone marrow microenvironment. in: Zeng, B. and Zhao, K. (ed.) Neurobiology of Chinese Herb Medicine Elsevier.
Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring
Antia, I., Yagnik, D., Pantoja Munoz, L., Shah, A. and Hills, F. 2017. Heparan sulfate disaccharide measurement from biological samples using pre-column derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry. 530, pp. 17-30. https://doi.org/10.1016/j.ab.2017.04.019
Biomimetic strategies for fracture repair: engineering the cell microenvironment for directed tissue formation
Vas, W., Shah, M., Al Hosni, R., Owen, H. and Roberts, S. 2017. Biomimetic strategies for fracture repair: engineering the cell microenvironment for directed tissue formation. Journal of Tissue Engineering. 8, pp. 1-14. https://doi.org/10.1177/2041731417704791
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, S., Acha, D., Shah, A., Hills, F., Roitt, I., Demosthenous, A. and Bayford, R. 2017. Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics. 92, pp. 482-488. https://doi.org/10.1016/j.bios.2016.10.077
Granulocyte macrophage colony-stimulating factor and interferon gamma act through different mechanisms in reversing post-operative immune suppression
Longbottom, E., Torrance, H., Owen, H., Hinds, C., Pearse, R. and O'Dywer, M. 2016. Granulocyte macrophage colony-stimulating factor and interferon gamma act through different mechanisms in reversing post-operative immune suppression. ESICM LIVES 2016: 29th Annual Congress of European Society of Intensive Care Medicine. Milan, Italy 01 - 05 Oct 2016 Springer. https://doi.org/10.1186/s40635-016-0098-x
Systemic inflammatory response syndrome after major abdominal surgery predicted by early upregulation of TLR4 and TLR5
Lahiri, R., Derwa, Y., Bashir, Z., Giles, E., Torrance, H., Owen, H., O'Dwyer, M., O'Brien, A., Stagg, A., Bhattacharya, S., Foster, G. and Alazawi, W. 2016. Systemic inflammatory response syndrome after major abdominal surgery predicted by early upregulation of TLR4 and TLR5. Annals of Surgery. 263 (5), pp. 1028-1037. https://doi.org/10.1097/SLA.0000000000001248
Features of postoperative immune suppression are reversible with interferon gamma and independent of interleukin-6 pathways
Longbottom, E., Torrance, H., Owen, H., Fragkou, P., Hinds, C., Pearse, R. and O'Dwyer, M. 2016. Features of postoperative immune suppression are reversible with interferon gamma and independent of interleukin-6 pathways. Annals of Surgery. 264 (2), pp. 370-377. https://doi.org/10.1097/SLA.0000000000001484
Association between early postoperatively elevated TLR-4 & TLR-5 gene expression and late nosocomial infection in elective high-risk hepato-pancreato-biliary (HPB) surgery
Pirmadjid, N., Torrance, H., Owen, H., Alazawi, W. and O'Dwyer, M. 2015. Association between early postoperatively elevated TLR-4 & TLR-5 gene expression and late nosocomial infection in elective high-risk hepato-pancreato-biliary (HPB) surgery. 2015 International Surgical Congress of the Association of Surgeons of Great Britain and Ireland. Manchester, UK 22 - 24 Apr 2015 Oxford University Press (OUP).
T-helper cell polarisation following severe polytrauma
Torrance, H., Brohi, K., Warnes, G., Owen, H., Hinds, C., Pennington, D. and O'Dwyer, M. 2015. T-helper cell polarisation following severe polytrauma. ESICM LIVES 2015: 28th Annual Congress of European Society of Intensive Care Medicine. Berlin, Germany 03 - 07 Oct 2015 Springer Open. https://doi.org/10.1186/2197-425X-3-S1-A848
Post-operative immune suppression is reversible with interferon gamma and independent of IL-6 pathways
Longbottom, E., Torrance, H., Owen, H., Hinds, C., Pearse, R. and O'Dwyer, M. 2015. Post-operative immune suppression is reversible with interferon gamma and independent of IL-6 pathways. ESICM LIVES 2015: 28th Annual Congress of European Society of Intensive Care Medicine. Berlin, Germany 03 - 07 Oct 2015 Springer Open. https://doi.org/10.1186/2197-425X-3-S1-A820
The role of micrornas in the development of hospital acquired infection in polytrauma patients
Owen, H., Torrance, H., Barnes, M., Brohi, K., Knight, J., Hinds, C. and O'Dwyer, M. 2015. The role of micrornas in the development of hospital acquired infection in polytrauma patients. ESICM LIVES 2015: 28th Annual Congress of European Society of Intensive Care Medicine. Berlin, Germany 03 - 07 Oct 2015 Springer Open. https://doi.org/10.1186/2197-425X-3-S1-A35
Changes in gene expression following trauma are related to the age of transfused packed red blood cells
Torrance, H., Vivian, M., Brohi, K., Prowle, J., Pearse, R., Owen, H., Hinds, C. and O'Dwyer, M. 2015. Changes in gene expression following trauma are related to the age of transfused packed red blood cells. Journal of Trauma and Acute Care Surgery. 78 (3), pp. 535-542. https://doi.org/10.1097/TA.0000000000000534
The perioperative immune response
O'Dwyer, M., Owen, H. and Torrance, H. 2015. The perioperative immune response. Current Opinion in Critical Care. 21 (4), pp. 336-342. https://doi.org/10.1097/MCC.0000000000000213
Critical illness-induced bone loss is related to deficient autophagy and histone hypomethylation
Owen, H., Vanhees, I., Gunst, J., Van Cromphaut, S. and Van den Berghe, G. 2015. Critical illness-induced bone loss is related to deficient autophagy and histone hypomethylation. Intensive Care Medicine Experimental. 3. https://doi.org/10.1186/s40635-015-0052-3
Epigenetic regulatory pathways involving microRNAs may modulate the host immune response following major trauma
Owen, H., Torrance, H., Jones, T., Pearse, R., Hinds, C., Brohi, K. and O'Dwyer, M. 2015. Epigenetic regulatory pathways involving microRNAs may modulate the host immune response following major trauma. Journal of Trauma and Acute Care Surgery. 79 (5), pp. 766-772. https://doi.org/10.1097/TA.0000000000000850
Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines
Busschots, S., O'Toole, S., O'Leary, J. and Stordal, B. 2015. Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines. MethodsX. 2, pp. 8-13. https://doi.org/10.1016/j.mex.2014.11.002
Identifying novel hypoxia-associated markers of chemoresistance in ovarian cancer
McEvoy, L., O'Toole, S., Spillane, C., Martin, C., Gallagher, M., Stordal, B., Blackshields, G., Sheils, O. and O'Leary, J. 2015. Identifying novel hypoxia-associated markers of chemoresistance in ovarian cancer. BMC cancer. 15 (1). https://doi.org/10.1186/s12885-015-1539-8
Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells
Busschots, S., O'Toole, S., O'Leary, J. and Stordal, B. 2015. Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells. Experimental Cell Research. 336 (1), pp. 1-14. https://doi.org/10.1016/j.yexcr.2014.12.001
Humanized culture of periosteal progenitors in allogeneic serum enhances osteogenic differentiation and in vivo bone formation
Roberts, S., Owen, H., Tam, W., Solie, L., Van Cromphaut, S., Van den Berghe, G. and Luyten, F. 2014. Humanized culture of periosteal progenitors in allogeneic serum enhances osteogenic differentiation and in vivo bone formation. Stem Cells Translational Medicine. 3 (2), pp. 218-28. https://doi.org/10.5966/sctm.2012-0137
Perioperative blood transfusion is associated with a gene transcription profile characteristic of immunosuppression: a prospective cohort study
Fragkou, P., Torrance, H., Pearse, R., Ackland, G., Prowle, J., Owen, H., Hinds, C. and O'Dwyer, M. 2014. Perioperative blood transfusion is associated with a gene transcription profile characteristic of immunosuppression: a prospective cohort study. Critical Care. 18 (5). https://doi.org/10.1186/s13054-014-0541-x
The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer
D’Adhemar, C., Spillane, C., Gallagher, M., Bates, M., Costello, K., Barry-O'Crowley, J., Haley, K., Kernan, N., Murphy, C., Smyth, P., O'Byrne, K., Pennington, S., Cooke, A., Ffrench, B., Martin, C., O'Donnell, D., Hennessy, B., Stordal, B., Finn, S., McCann, A., Gleeson, N., D'Arcy, T., Flood, B., O'Neill, L., Sheils, O., O'Toole, S. and O'Leary, J. 2014. The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer. PLoS ONE. 9 (6), pp. 1-15. https://doi.org/10.1371/journal.pone.0100816
OvMark: a user-friendly system for the identification of prognostic biomarkers in publically available ovarian cancer gene expression datasets
Madden, S., Clarke, C., Stordal, B., Carey, M., Broaddus, R., Gallagher, W., Crown, J., Mills, G. and Hennessy, B. 2014. OvMark: a user-friendly system for the identification of prognostic biomarkers in publically available ovarian cancer gene expression datasets. Molecular Cancer. 13 (1), pp. 1-11. https://doi.org/10.1186/1476-4598-13-241
PARP inhibitors as P-glyoprotein substrates
Lawlor, D., Martin, P., Busschots, S., Thery, J., O'Leary, J.J., Hennessy, B.T. and Stordal, B. 2014. PARP inhibitors as P-glyoprotein substrates. Journal of Pharmaceutical Sciences. 103 (6), pp. 1913-1920. https://doi.org/10.1002/jps.23952
Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells
Doherty, B., Lawlor, D., Gillet, J., Gottesman, M., O'Leary, J. and Stordal, B. 2014. Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells. Anticancer Research. 34 (1), pp. 503-507.
In vitro development of chemotherapy and targeted therapy drug-resistant cancer cell lines: a practical guide with case studies
McDermott, M., Eustace, A., Busschots, S., Breen, L., Crown, J., Clynes, M., O'Donovan, N. and Stordal, B. 2014. In vitro development of chemotherapy and targeted therapy drug-resistant cancer cell lines: a practical guide with case studies. Frontiers in Oncology. 4, pp. 1-16. https://doi.org/10.3389/fonc.2014.00040
Placental syndecan-1 and sulphated glycosaminoglycans are decreased in preeclampsia
Heyer-Chauhan, N., Ovbude, I., Hills, A., Sullivan, M. and Hills, F. 2013. Placental syndecan-1 and sulphated glycosaminoglycans are decreased in preeclampsia. Journal of Perinatal Medicine. https://doi.org/10.1515/jpm-2013-0097
IGFBP-1 in the placenta, membranes and fetal circulation: levels at term and preterm delivery
Hills, F., Gunn, L., Hardiman, P., Thanaratnam, S. and Chard, T. 1996. IGFBP-1 in the placenta, membranes and fetal circulation: levels at term and preterm delivery. Early Human Development. 44 (1), pp. 71-76.
Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy
Hills, F., Iles, R. and Sullivan, M. 2013. Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy. Journal of Perinatal Medicine. 41 (3), pp. 241-249. https://doi.org/10.1515/jpm-2012-0086
Enhanced immunoreceptor tyrosine-based activation motif signaling is related to pathological bone resorption during critical illness
Vanhees, I., Gunst, J., Janssens, T., Wauters, A., Van Herck, E., Van Cromphaut, S., Van den Berghe, G. and Owen, H. 2013. Enhanced immunoreceptor tyrosine-based activation motif signaling is related to pathological bone resorption during critical illness. Hormone and Metabolic Research. 45 (12), pp. 862-9. https://doi.org/10.1055/s-0033-1351290
BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation
Stordal, B., Timms, K., Farrelly, A., Gallagher, D., Busschots, S., Renaud, M., Thery, J., Williams, D., Potter, J., Tran, T., Korpanty, G., Cremona, M., Carey, M., Li, J., Li, Y., Aslan, O., O'Leary, J., Mills, G. and Hennessy, B. 2013. BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation. Molecular Oncology. 7 (3), pp. 567-79. https://doi.org/10.1016/j.molonc.2012.12.007
Insulin-like growth factor-II and heparin are anti-apoptotic survival factors in human villous cytotrophoblast
Hills, F., Mehmet, H. and Sullivan, M. 2012. Insulin-like growth factor-II and heparin are anti-apoptotic survival factors in human villous cytotrophoblast. European Journal of Obstetrics, Gynecology, and Reproductive Biology. 163 (1), pp. 11-16. https://doi.org/10.1016/j.ejogrb.2012.03.013
Critical illness-related bone loss is associated with osteoclastic and angiogenic abnormalities
Owen, H., Vanhees, I., Solie, L., Roberts, S., Wauters, A., Luyten, F., Van Cromphaut, S. and Van den Berghe, G. 2012. Critical illness-related bone loss is associated with osteoclastic and angiogenic abnormalities. Journal of Bone and Mineral Research. 27 (7), pp. 1541-1552. https://doi.org/10.1002/jbmr.1612
Resistance to paclitaxel in a cisplatin-resistant ovarian cancer cell line is mediated by P-glycoprotein
Stordal, B., Hamon, M., McEneaney, V., Roche, S., Gillet, J., O'Leary, J., Gottesman, M. and Clynes, M. 2012. Resistance to paclitaxel in a cisplatin-resistant ovarian cancer cell line is mediated by P-glycoprotein. PLoS ONE. 7 (7), pp. 1-13. https://doi.org/10.1371/journal.pone.0040717
Hypermethylation of CpG islands and shores around specific microRNAs and mirtrons is associated with the phenotype and presence of bladder cancer
Dudziec, E., Miah, S., Choudhry, H., Owen, H., Blizard, S., Glover, M., Hamdy, F. and Catto, J. 2011. Hypermethylation of CpG islands and shores around specific microRNAs and mirtrons is associated with the phenotype and presence of bladder cancer. Clinical Cancer Research. 17 (6), pp. 1287-1296. https://doi.org/10.1158/1078-0432.CCR-10-2017
Erlotinib or gefitinib for the treatment of relapsed platinum pretreated non-small cell lung cancer and ovarian cancer: a systematic review
Murphy, M. and Stordal, B. 2011. Erlotinib or gefitinib for the treatment of relapsed platinum pretreated non-small cell lung cancer and ovarian cancer: a systematic review. Drug Resistance Updates. 14 (3), pp. 177-190. https://doi.org/10.1016/j.drup.2011.02.004
Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells
Egan, K., Crowley, D., Smyth, P., O'Toole, S., Spillane, C., Martin, C., Gallagher, M., Canney, A., Norris, L., Conlon, N., McEvoy, L., Ffrench, B., Stordal, B., Keegan, H., Finn, S., McEneaney, V., Laios, A., Ducrée, J., Dunne, E., Smith, L., Berndt, M., Sheils, O., Kenny, D. and O'Leary, J. 2011. Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells. PLoS ONE. 6 (10). https://doi.org/10.1371/journal.pone.0026125
Superovulation, IGFBP-1 and birth weight.
Johnson, M., Irvine, R., Hills, F., Bolton, V., Abbas, A., Brooks, A., Allman, A., Chard, T. and Nicolaides, K. 1995. Superovulation, IGFBP-1 and birth weight. European Journal of Obstetrics, Gynaecology and Reproductive Biology. 59 (2), pp. 193-195.
The effects of labor on maternal and fetal levels of insulin-like growth factor binding protein-1
Hills, F., Crawford, R., Harding, S., Farkas, F. and Chard, T. 1994. The effects of labor on maternal and fetal levels of insulin-like growth factor binding protein-1. American Journal of Obstetrics and Gynecology. 171 (5), pp. 1292-1295.
Insulin-related growth factor binding protein-1 levels in ovum donation pregnancies.
Brooks, A., Johnson, M., Hills, F., Chard, T., Irvine, R. and Abdalla, H. 1995. Insulin-related growth factor binding protein-1 levels in ovum donation pregnancies. European Journal of Obstetrics, Gynaecology and Reproductive Biology. 59 (1), pp. 91-94.
Elevated levels of insulin-like growth factor binding protein-1 in fetal distress.
Crawford, R., Hills, F., Farkas, A. and Chard, T. 1995. Elevated levels of insulin-like growth factor binding protein-1 in fetal distress. British Journal of Obstetrics and Gynaecology. 102 (7), pp. 538-540.
Lack of evidence for a circadian rhythm of IGFBP-1 in the mother and fetus during labour.
Harding, S., Mensah, A., Hills, F., Howell, R. and Chard, T. 1995. Lack of evidence for a circadian rhythm of IGFBP-1 in the mother and fetus during labour. British Journal of Obstetrics and Gynaecology. 102 (11), pp. 891-893.
Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device.
Wolley, J., Saleem, S., Hills, F., Salem, H., El-Nashar, E. and Chard, T. 1996. Raised circulating levels of interleukin-6 in women with an intrauterine contraceptive device. Gynecologic and Obstetric Investigation. 42 (4), pp. 241-243.
Circulating levels of IGF-I and IGF-binding protein-1 throughout pregnancy: relation to birthweight and maternal weight.
Hills, F., English, J. and Chard, T. 1996. Circulating levels of IGF-I and IGF-binding protein-1 throughout pregnancy: relation to birthweight and maternal weight. Journal of Endocrinology. 148 (2), pp. 303-309. https://doi.org/10.1677/joe.0.1480303
Antenatal dexamethasone therapy does not affect circulating concentrations of insulin-like growth factor binding protein-1.
Ogueh, O., Hills, F., Chard, T. and Johnson, M. 1998. Antenatal dexamethasone therapy does not affect circulating concentrations of insulin-like growth factor binding protein-1. Human Reproduction. 13 (6), pp. 1714-1716. https://doi.org/10.1093/humrep/13.6.1714
Effects of co-trimoxazole, erythromycin, amoxycillin, tetracycline and chloroquine on sperm function in vitro
Hargreaves, C., Rogers, S., Hills, F., Rahman, F., Howell, R. and Homa, S. 1998. Effects of co-trimoxazole, erythromycin, amoxycillin, tetracycline and chloroquine on sperm function in vitro. Human Reproduction. 13 (7), pp. 1878-1886.
Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression
Monk, D., Arnaud, P., Frost, J., Hills, F., Stanier, P., Feil, R. and Moore, G. 2009. Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression. Human Molecular Genetics. 18 (16), pp. 3066-3074. https://doi.org/10.1093/hmg/ddp248
Low frequency of epigenetic events in urothelial tumours from young patients
Catto, J., Owen, H., Giedl, J., Wild, P., Fine, S., Epstein, J., Humphrey, P., Dehner, L., Amin, M., Blaszyk, H., Hughes, D., Hartmann, A. and Stoehr, R. 2010. Low frequency of epigenetic events in urothelial tumours from young patients. European Urology Supplements. 9 (2), pp. 260-261. https://doi.org/10.1016/S1569-9056(10)60798-1
Low frequency of epigenetic events in urothelial tumors in young patients
Owen, H., Giedl, J., Wild, P., Fine, S., Humphrey, P., Dehner, L., Amin, M., Epstein, J., Blaszyk, H., Hughes, D., Hartmann, A., Stoehr, R. and Catto, J. 2010. Low frequency of epigenetic events in urothelial tumors in young patients. The Journal of Urology. 184 (2), pp. 459-463. https://doi.org/10.1016/j.juro.2010.03.131
Heparin prevents programmed cell death in human trophoblast
Hills, F., Abrahams, V., Gonzalez-Timon, B., Francis, J., Cloke, B., Hinkson, L., Rai, R., Regan, L., Mor, G., Sullivan, M., Lam, E. and Brosens, J. 2006. Heparin prevents programmed cell death in human trophoblast. Molecular Human Reproduction. 12 (4), pp. 237-242.
A longitudinal study of maternal plasma insulin-like growth factor binding protein-1 concentrations during normal pregnancy and pregnancies complicated by pre-eclampsia
Anim-Nyame, N., Hills, F., Soorana, S., Steer, P. and Johnson, M. 2000. A longitudinal study of maternal plasma insulin-like growth factor binding protein-1 concentrations during normal pregnancy and pregnancies complicated by pre-eclampsia. Human Reproduction. 15 (10), pp. 2215-2219. https://doi.org/10.1093/humrep/15.10.2215
Interactions between progesterone receptor isoforms in myometrial cells in human labour
Pieber, D., Allport, V., Hills, F., Johnson, M. and Bennett, P. 2001. Interactions between progesterone receptor isoforms in myometrial cells in human labour. Molecular Human Reproduction. 7 (9), pp. 875-879. https://doi.org/10.1093/molehr/7.9.875
Regulated expression of signal transducer and activator of transcription, Stat5, and its enhancement of PRL expression in human endometrial stromal cells in vitro
Mak, I., Brosens, J., Christian, M., Hills, F., Chamley, L., Regan, L. and White, J. 2002. Regulated expression of signal transducer and activator of transcription, Stat5, and its enhancement of PRL expression in human endometrial stromal cells in vitro. Journal of Clinical Endocrinology and Metabolism. 87 (6), pp. 2581-2588. https://doi.org/10.1210/jc.87.6.2581
Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells
Loudon, J., Elliott, C., Hills, F. and Bennett, P. 2003. Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells. Biology of Reproduction. 69 (1), pp. 331-337.
The relationship between insulin and insulin-like growth factor binding protein-1 is modified by pre-eclampsia.
Anim-Nyame, N., Hills, F., Soorana, S., Steer, P. and Johnson, M. 2003. The relationship between insulin and insulin-like growth factor binding protein-1 is modified by pre-eclampsia. Gynecological endocrinology.. 17 (6), pp. 471-476. https://doi.org/10.1080/09513590312331290408
The insulin-like growth factor system in mammalian pregnancy: a workshop report
Carter, A., Hills, F., O'Gorman, D., Robets, C., Soorana, S., Watson, C. and Westwood, M. 2004. The insulin-like growth factor system in mammalian pregnancy: a workshop report. Placenta. 25 (Supple), pp. S53-S56. https://doi.org/10.1016/j.placenta.2004.01.019
Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human
Monk, D., Sanches, R., Arnaud, P., Apostolidou, S., Hills, F., Abu-Amero, S., Murrell, A., Friess, H., Reik, W. and Stanier, P. 2006. Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human. Human Molecular Genetics. 15 (8), pp. 1259-1269. https://doi.org/10.1093/hmg/ddl041
Chondrocyte p21(WAF1/CIP1) expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo
Owen, H., Ahmed, S. and Farquharson, C. 2009. Chondrocyte p21(WAF1/CIP1) expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo. Calcified Tissue International and Musculoskeletal Research. 85 (4), pp. 326-334. https://doi.org/10.1007/s00223-009-9276-0
Distinct microRNA alterations characterize high- and low-grade bladder cancer
Catto, J., Miah, S., Owen, H., Bryant, H., Myers, K., Dudziec, E., Larré, S., Milo, M., Rehman, I., Rosario, D., Di Martino, E., Knowles, M., Meuth, M., Harris, A. and Hamdy, F. 2009. Distinct microRNA alterations characterize high- and low-grade bladder cancer. Cancer Research. 69 (21), pp. 8472-8481. https://doi.org/10.1158/0008-5472.CAN-09-0744
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair
Stordal, B. and Davey, R. 2009. ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair. Cancer Chemotherapy and Pharmacology. 63 (4), pp. 661-672. https://doi.org/10.1007/s00280-008-0783-x
Citations, citations everywhere but did anyone read the paper? [Letter to the Editor]
Stordal, B. 2009. Citations, citations everywhere but did anyone read the paper? [Letter to the Editor]. Colloids and surfaces. B, Biointerfaces. 72 (2), pp. 312 -312. https://doi.org/10.1016/j.colsurfb.2009.04.001
A systematic review of genes involved in the inverse resistance relationship between cisplatin and paclitaxel chemotherapy: role of BRCA1
Stordal, B. and Davey, R. 2009. A systematic review of genes involved in the inverse resistance relationship between cisplatin and paclitaxel chemotherapy: role of BRCA1. Current Cancer Drug Targets. 9 (3), pp. 354-365. https://doi.org/10.2174/156800909788166592
STOX1 is not imprinted and is not likely to be involved in preeclampsia.
Hills, F., Iglesias-Platas, I., Jebbink, J. and Monk, D. 2007. STOX1 is not imprinted and is not likely to be involved in preeclampsia. Nature Genetics. 39 (3), pp. 279-280. https://doi.org/10.1038/ng0307-279
Limited evolutionary conservation of imprinting in the human placenta
Hills, F., Apostolidou, S., Arnaud, P. and Monk, D. 2006. Limited evolutionary conservation of imprinting in the human placenta. Proceedings of the National Academy of Sciences. 103 (17), pp. 6623-6628. https://doi.org/10.1073/pnas.0511031103
Dexamethasone-induced expression of the glucocorticoid response gene lipocalin 2 in chondrocytes
Owen, H., Roberts, S., Ahmed, S. and Farquharson, C. 2008. Dexamethasone-induced expression of the glucocorticoid response gene lipocalin 2 in chondrocytes. American Journal of Physiology - Endocrinology and Metabolism. 294 (6), pp. E1023-E1034. https://doi.org/10.1152/ajpendo.00586.2007
Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1
Roberts, S., Owen, H. and Farquharson, C. 2008. Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1. Biochemical and Biophysical Research Communications. 371 (4), pp. 872-876. https://doi.org/10.1016/j.bbrc.2008.04.163
Platinum resistance needs the mythbusters [Letter to the Editor]
Stordal, B. and Davey, R. 2008. Platinum resistance needs the mythbusters [Letter to the Editor]. Toxicology letters. 180 (3), p. 230. https://doi.org/10.1016/j.toxlet.2008.07.003
A 39 kDa fragment of endogenous ASK1 suggests specific cleavage not degradation by the proteasome
Stordal, B. and Davey, R. 2008. A 39 kDa fragment of endogenous ASK1 suggests specific cleavage not degradation by the proteasome. IUBMB Life. 60 (3), pp. 180-184. https://doi.org/10.1002/iub.24
Identification and functional involvement of lipocalin 2 in glucocorticoid-induced growth retardation
Owen, H., Ahmed, S. and Farquharson, C. 2007. Identification and functional involvement of lipocalin 2 in glucocorticoid-induced growth retardation. 29th Annual Meeting of the American Society for Bone and Mineral Research. Honolulu, HI 16 - 19 Sep 2007 Wiley.
The role of P21CIP1/WAF1 in glucocorticoid induced growth retardation
Owen, H., Ahmed, S. and Farquharson, C. 2007. The role of P21CIP1/WAF1 in glucocorticoid induced growth retardation. 2007 Annual Meeting of the Bone Research Society. Southampton, UK 03 - 05 Jul 2007 Wiley.
Identification of lipocalin 2, a novel glucocorticoid responsive gene in growth plate chondrocytes
Owen, H., Ahmed, S. and Farquharson, C. 2007. Identification of lipocalin 2, a novel glucocorticoid responsive gene in growth plate chondrocytes. 2007 Annual Meeting of the Bone Research Society. Southampton, UK 03 - 05 Jul 2007 Wiley.
The growth plate sparing effects of the selective glucocorticoid receptor modulator, AL-438
Owen, H., Miner, J., Ahmed, S. and Farquharson, C. 2007. The growth plate sparing effects of the selective glucocorticoid receptor modulator, AL-438. Molecular and Cellular Endocrinology. 264 (1-2), pp. 164-70. https://doi.org/10.1016/j.mce.2006.11.006
A systematic review of platinum and taxane resistance from bench to clinic: an inverse relationship
Stordal, B., Pavlakis, N. and Davey, R. 2007. A systematic review of platinum and taxane resistance from bench to clinic: an inverse relationship. Cancer Treatment Reviews. 33 (8), pp. 688-703. https://doi.org/10.1016/j.ctrv.2007.07.013
Understanding cisplatin resistance using cellular models
Stordal, B. and Davey, M. 2007. Understanding cisplatin resistance using cellular models. IUBMB Life. 59 (11), pp. 696-699. https://doi.org/10.1080/15216540701636287
Oxaliplatin for the treatment of cisplatin-resistant cancer: a systematic review
Stordal, B., Pavlakis, N. and Davey, R. 2007. Oxaliplatin for the treatment of cisplatin-resistant cancer: a systematic review. Cancer Treatment Reviews. 33 (4), pp. 347-357. https://doi.org/10.1016/j.ctrv.2007.01.009
Glucocorticoids stimulate p21 expression in growth plate chondrocytes
Owen, H., Ahmed, S. and Farquharson, C. 2006. Glucocorticoids stimulate p21 expression in growth plate chondrocytes. 2006 Annual Meeting of the Bone Research Society. Southampton, UK 05 - 06 Jul 2006 Wiley.
Glucocorticoids induce lipocalin 2 gene expression in growth plate chondrocytes.
Owen, H., Ahmed, S. and Farquharson, C. 2006. Glucocorticoids induce lipocalin 2 gene expression in growth plate chondrocytes. 28th Annual Meeting of the American Society for Bone and Mineral Research. Philadelphia, PA 15 - 19 Sep 2006 Wiley.
The role of the 11 beta HSD shuttle in modulating the effects of proinflammatory cytokines on the growth plate
MacRae, V., Owen, H., Ahmed, S. and Farquharson, C. 2006. The role of the 11 beta HSD shuttle in modulating the effects of proinflammatory cytokines on the growth plate. 28th Annual Meeting of the American Society for Bone and Mineral Research. Philadelphia, PA 15 - 19 Sep 2006 Wiley.
Similar chromosomal changes in cisplatin and oxaliplatin-resistant sublines of the H69 SCLC cell line are not associated with platinum resistance
Stordal, B., Peters, G. and Davey, R. 2006. Similar chromosomal changes in cisplatin and oxaliplatin-resistant sublines of the H69 SCLC cell line are not associated with platinum resistance. Genes, Chromosomes & Cancer. 45 (12), pp. 1094-105. https://doi.org/10.1002/gcc.20373
Oxaliplatin induces drug resistance more rapidly than cisplatin in H69 small cell lung cancer cells
Stordal, B., Davey, M. and Davey, R. 2006. Oxaliplatin induces drug resistance more rapidly than cisplatin in H69 small cell lung cancer cells. Cancer Chemotherapy and Pharmacology. 58 (2), pp. 256-265. https://doi.org/10.1007/s00280-005-0148-7
Regulation of human villous trophoblast by insulin-like growth factors and insulin-like growth factor-binding protein-1
Hills, F., Chard, T., Elder, M. and Sullivan, M. 2004. Regulation of human villous trophoblast by insulin-like growth factors and insulin-like growth factor-binding protein-1. Journal of Endocrinology. 183 (3), pp. 487-496. https://doi.org/10.1677/joe.1.05867
Polyclonal anti-tumor necrosis factor-alpha Fab used as an ancillary treatment for severe malaria
Looareesuwan, S., Sjorstrom, L., Krudsood, S., Wilairatana, P., Porter, R., Hills, F. and Warrell, D. 1999. Polyclonal anti-tumor necrosis factor-alpha Fab used as an ancillary treatment for severe malaria. The American Journal of Tropical Medicine and Hygiene. 61 (1), pp. 26-33. https://doi.org/10.4269/ajtmh.1999.61.26
Mechanism of action of the intrauterine contraceptive device: evidence for a specific biochemical deficiency in the endometrium
Seleem, S., Hills, F., Salem, H., El-Nashar, E. and Chard, T. 1996. Mechanism of action of the intrauterine contraceptive device: evidence for a specific biochemical deficiency in the endometrium. Human Reproduction. 11 (6), pp. 1220-1222.