The combination of curcumin and doxorubicin shows a selective cytotoxic effect on acute myeloid leukaemia cell line

Conference poster


Hasan, N., Roberts, H., Ghali, L. and Appiah, S. 2018. The combination of curcumin and doxorubicin shows a selective cytotoxic effect on acute myeloid leukaemia cell line. NCRI Cancer Conference (2018). Glasgow, Scotland 04 - 06 Nov 2018
TypeConference poster
TitleThe combination of curcumin and doxorubicin shows a selective cytotoxic effect on acute myeloid leukaemia cell line
AuthorsHasan, N., Roberts, H., Ghali, L. and Appiah, S.
Abstract

Background
Chemotherapy is the frontline treatment for acute myeloid leukaemia (AML), however, due to clinical limitations, including cardiotoxicity and drug resistance, drug-free survival rarely goes beyond 5 years; therefore natural products are increasingly being studied as potential therapeutic agents. Curcuma longa (curcumin), a component of turmeric, has shown to induce anti-oxidant and anti-inflammatory properties. This study investigated the effect of curcumin and doxorubicin individually and as combinations on healthy human monocyte cells and AML (MOLM13) cell line.
Method
Cell viability was measured using CyQuant assay (binds to nucleic acids of live cells), and the appropriate compound concentrations were selected for drug combination studies for 24 h and 48 h incubation periods. The effect of single and combination incubations on Bcl-2 (anti-apoptotic) protein was also investigated using Western blot.
Results
Neither doxorubicin (0.1-0.5 µM) nor curcumin (5-15 µM) incubation of normal monocyte cells showed any significant decrease in cell viability compared to negative control at 24 h and 48 h. AML cells incubated with either doxorubicin or curcumin showed the compounds act in a dose and time-dependent manner. This demonstrates curcumin to possess selective cytotoxic properties against the MOLM13 cell line and not the normal monocyte cells. The combination of doxorubicin (0.2 and 0.5 µM) with curcumin (10µM) also decreased the cell viability more pronouncedly in MOLM13 compared to normal monocytes. Curcumin (10 µM) and doxorubicin (0.2 µM) combination showed a 1.4 fold reduction in Bcl-2 protein levels (p=0.002), as compared to doxorubicin 0.2 µM alone.
Conclusion
It is thought that the combination of curcumin with doxorubicin has the potential to increase the therapeutic effect of chemotherapy while also reducing its cytotoxicity.
Helen 1,Lucy 1,Sandra Appiah1

Research GroupBiomarkers for Cancer group
LanguageEnglish
ConferenceNCRI Cancer Conference (2018)
Publication dates
Print06 Nov 2018
Publication process dates
Deposited29 Jan 2019
Completed06 Nov 2018
Accepted01 Jul 2018
Output statusPublished
Web address (URL)http://abstracts.ncri.org.uk/abstract/the-combination-of-curcumin-and-doxorubicin-shows-a-selective-cytotoxic-effect-on-acute-myeloid-leukaemia-cell-line/
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GLI1 confers profound phenotypic changes upon LNCaP prostate cancer cells that include the acquisition of a hormone independent state
Nadenla, S., Hazan, A., Ward, M., Harper, L., Moutasim, K., Bianchi, L., Naase, M., Ghali, L., Thomas, G., Prowse, D., Philpott, M. and Neill, G. 2011. GLI1 confers profound phenotypic changes upon LNCaP prostate cancer cells that include the acquisition of a hormone independent state. PLoS ONE. 6 (5), pp. 1-13. https://doi.org/10.1371/journal.pone.0020271
Evaluation of herbs as potential alternatives for bear bile and rhino horn used in traditional Chinese medicines: chemical and biological analysis.
Appiah, S. 2006. Evaluation of herbs as potential alternatives for bear bile and rhino horn used in traditional Chinese medicines: chemical and biological analysis. PhD thesis Middlesex University School of Health and Social Sciences
Hypermethylation of CpG islands and shores around specific microRNAs and mirtrons is associated with the phenotype and presence of bladder cancer
Dudziec, E., Miah, S., Choudhry, H., Owen, H., Blizard, S., Glover, M., Hamdy, F. and Catto, J. 2011. Hypermethylation of CpG islands and shores around specific microRNAs and mirtrons is associated with the phenotype and presence of bladder cancer. Clinical Cancer Research. 17 (6), pp. 1287-1296. https://doi.org/10.1158/1078-0432.CCR-10-2017
Investigation of the safety and efficacy of Chinese herbs traditionally used to treat inflammatory diseases
Appiah, S., Kokubun, T., Bremner, P., Heinrich, M., Bell, C. and Simmonds, M. 2003. Investigation of the safety and efficacy of Chinese herbs traditionally used to treat inflammatory diseases. 10th Annual Symposium on Complementary Health Care. London, UK 21 - 22 Nov 2003
Herbal alternatives to bear bile: effects of scutellaria baicalensis Georgi on IL-6 promoter and CYP3A4 activities.
Appiah, S., Bremner, P., Kokubun, T., Russell, M., Bell, C., Heinrich, M. and Simmonds, M. 2006. Herbal alternatives to bear bile: effects of scutellaria baicalensis Georgi on IL-6 promoter and CYP3A4 activities. 13th Annual Symposium on Complementary Health Care. Exeter, UK 12 - 14 Dec 2006
Plant substances as alternatives for animal products in traditional medicines.
Bell, C., Simmonds, M., Appiah, S. and Howes, M. 2006. Plant substances as alternatives for animal products in traditional medicines. London Department for the Environment, Food and Rural Affairs.
Low frequency of epigenetic events in urothelial tumours from young patients
Catto, J., Owen, H., Giedl, J., Wild, P., Fine, S., Epstein, J., Humphrey, P., Dehner, L., Amin, M., Blaszyk, H., Hughes, D., Hartmann, A. and Stoehr, R. 2010. Low frequency of epigenetic events in urothelial tumours from young patients. European Urology Supplements. 9 (2), pp. 260-261. https://doi.org/10.1016/S1569-9056(10)60798-1
Low frequency of epigenetic events in urothelial tumors in young patients
Owen, H., Giedl, J., Wild, P., Fine, S., Humphrey, P., Dehner, L., Amin, M., Epstein, J., Blaszyk, H., Hughes, D., Hartmann, A., Stoehr, R. and Catto, J. 2010. Low frequency of epigenetic events in urothelial tumors in young patients. The Journal of Urology. 184 (2), pp. 459-463. https://doi.org/10.1016/j.juro.2010.03.131
Laser modulation of angiogenic factor production by T-lymphocytes.
Agaiby, A., Ghali, L., Wilson, R. and Dyson, M. 2000. Laser modulation of angiogenic factor production by T-lymphocytes. Lasers in surgery and medicine. 26 (4), pp. 357-363. https://doi.org/10.1002/(SICI)1096-9101(2000)26:4<357::AID-LSM3>3.0.CO;2-O
An association between sebaceous carcinoma and microsatellite instability in immunosuppressed organ transplant recipients
Harwood, C., Swale, V., Bataille, V., Quinn, A., Ghali, L., Patel, S., Dove-Edwin, I., Cerio, R. and McGregor, J. 2001. An association between sebaceous carcinoma and microsatellite instability in immunosuppressed organ transplant recipients. Journal of investigative dermatology. 116 (2), pp. 246-253. https://doi.org/10.1046/j.1523-1747.2001.01233.x
Human β defensin-1 and -2 expression in human pilosebaceous units: upregulation in acne vulgaris lesions
Chronnell, C., Ghali, L., Ali, R., Quinn, A., Holland, D., Bull, J., Cunliffe, W., McKay, I., Philpott, M. and Müller-Röver, S. 2001. Human β defensin-1 and -2 expression in human pilosebaceous units: upregulation in acne vulgaris lesions. Journal of investigative dermatology. 117 (5), pp. 1120-1125. https://doi.org/10.1046/j.0022-202x.2001.01569.x
Reduced apoptotic levels in squamous but not basal cell carcinomas correlates with detection of cutaneous human papillomavirus
Jackson, S., Ghali, L., Harwood, C. and Storey, A. 2002. Reduced apoptotic levels in squamous but not basal cell carcinomas correlates with detection of cutaneous human papillomavirus. British journal of cancer. 87 (3), pp. 319-323. https://doi.org/10.1038/sj.bjc.6600431
Expression of keratin K2e in cutaneous and oral lesions: association with keratinocyte activation, proliferation, and keratinization
Bloor, B., Tidman, N., Leigh, I., Odell, E., Dogan, B., Wollina, U., Ghali, L. and Waseem, A. 2003. Expression of keratin K2e in cutaneous and oral lesions: association with keratinocyte activation, proliferation, and keratinization. American Journal of Pathology. 162 (3), pp. 963-975.
The E7 protein of cutaneous human papillomavirus type 8 causes invasion of human keratinocytes into the dermis in organotypic cultures of skin
Akgul, B., Garcia-Escudero, R., Ghali, L., Pfister, H., Fuchs, P., Navsaria, H. and Storey, A. 2005. The E7 protein of cutaneous human papillomavirus type 8 causes invasion of human keratinocytes into the dermis in organotypic cultures of skin. Cancer Research. 65 (6), pp. 2216-2223. https://doi.org/10.1158/0008-5472.CAN-04-1952
LEKTI demonstrable by immunohistochemistry of the skin: a potential diagnostic skin test for Netherton syndrome.
Ong, C., O'Toole, E., Ghali, L., Malone, M., Smith, V., Callard, R. and Harper, J. 2004. LEKTI demonstrable by immunohistochemistry of the skin: a potential diagnostic skin test for Netherton syndrome. British journal of dermatology. 151 (6), pp. 1253-1257. https://doi.org/10.1111/j.1365-2133.2004.06180.x
Safety and efficacy of 5% imiquimod cream for the treatment of skin dysplasia in high-risk renal transplant recipients: randomized, double-blind, placebo-controlled trial
Brown, V., Atkins, C., Ghali, L., Cerio, R., Harwood, C. and Proby, C. 2005. Safety and efficacy of 5% imiquimod cream for the treatment of skin dysplasia in high-risk renal transplant recipients: randomized, double-blind, placebo-controlled trial. Archives of Dermatology. 141 (8), pp. 985-993.
Overexpression of the Axl tyrosine kinase receptor in cutaneous SCC-derived cell lines and tumours
Green, J., Ikram, M., Vyas, J., Patel, N., Proby, C., Ghali, L., Leigh, I., O'Toole, E. and Storey, A. 2006. Overexpression of the Axl tyrosine kinase receptor in cutaneous SCC-derived cell lines and tumours. British journal of cancer. 94 (10), pp. 1446-1451.
Role of HPV E6 proteins in preventing UVB-induced release of pro-apoptotic factors from the mitochondria.
Leverrier, S., Bergamaschi, D., Ghali, L., Ola, A., Warnes, G., Akgul, B., Blight, K., Garcia-Escudero, R., Penna, A., Eddaoudi, A. and Storey, A. 2007. Role of HPV E6 proteins in preventing UVB-induced release of pro-apoptotic factors from the mitochondria. Apoptosis: an international journal on programmed cell death. 12 (3), pp. 549-560. https://doi.org/10.1007/s10495-006-0004-1
Role for WNT16B in human epidermal keratinocyte proliferation and differentiation
Teh, M., Blaydon, D., Ghali, L., Briggs, V., Edmunds, S., Pantazi, E., Barnes, M., Leigh, I., Kelsell, D. and Philpott, M. 2007. Role for WNT16B in human epidermal keratinocyte proliferation and differentiation. Journal of Cell Science. 120 (2), pp. 330-339.
Longitudinal muscle shows abnormal relaxation responses to nitric oxide and contains altered levels of NOS1 and elastin in uncomplicated diverticular disease.
Golder, M., Burleigh, D., Ghali, L., Feakins, R., Lunniss, P., Williams, N. and Navsaria, H. 2007. Longitudinal muscle shows abnormal relaxation responses to nitric oxide and contains altered levels of NOS1 and elastin in uncomplicated diverticular disease. Colorectal disease. 9 (3), pp. 218-228.
HPV8 early genes modulate differentiation and cell cycle of primary human adult keratinocytes.
Akgul, B., Ghali, L., Davies, D., Pfister, H., Leigh, I. and Storey, A. 2007. HPV8 early genes modulate differentiation and cell cycle of primary human adult keratinocytes. Clinical and experimental dermatology. 16 (7), pp. 590-599.
Granulosa cell survival and proliferation are altered in polycystic ovary syndrome
Das, M., Djahanbakhch, O., Hacihanefioglu, B., Saridogan, E., Ikram, M., Ghali, L., Raveendran, M. and Storey, A. 2008. Granulosa cell survival and proliferation are altered in polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism. 93 (3), pp. 881-887. https://doi.org/10.1210/jc.2007-1650
GLI1 repression of ERK activity correlates with colony formation and impaired migration in human epidermal keratinocytes
Neill, G., Harrison, W., Ikram, M., Williams, T., Bianchi, L., Nadenla, S., Green, J., Ghali, L., Frischauf, A., O'Toole, E., Aberger, F. and Philpott, M. 2008. GLI1 repression of ERK activity correlates with colony formation and impaired migration in human epidermal keratinocytes. Carcinogenesis. 29 (4), pp. 738-746. https://doi.org/10.1093/carcin/bgn037
Gonadotropins and prostate cancer: revisited.
Thwaini, A., Naase, M., Chinegwundon, F., Baithun, S., Ghali, L., Shergill, I. and Iles, R. 2006. Gonadotropins and prostate cancer: revisited. Urologia internationalis. 77 (4), pp. 289-296. https://doi.org/10.1159/000096330
Chondrocyte p21(WAF1/CIP1) expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo
Owen, H., Ahmed, S. and Farquharson, C. 2009. Chondrocyte p21(WAF1/CIP1) expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo. Calcified Tissue International and Musculoskeletal Research. 85 (4), pp. 326-334. https://doi.org/10.1007/s00223-009-9276-0
Distinct microRNA alterations characterize high- and low-grade bladder cancer
Catto, J., Miah, S., Owen, H., Bryant, H., Myers, K., Dudziec, E., Larré, S., Milo, M., Rehman, I., Rosario, D., Di Martino, E., Knowles, M., Meuth, M., Harris, A. and Hamdy, F. 2009. Distinct microRNA alterations characterize high- and low-grade bladder cancer. Cancer Research. 69 (21), pp. 8472-8481. https://doi.org/10.1158/0008-5472.CAN-09-0744
Smooth muscle cholinergic denervation hypersensitivity in diverticular disease.
Ghali, L., Belai, A., Burleigh, D. and Golder, M. 2003. Smooth muscle cholinergic denervation hypersensitivity in diverticular disease. The Lancet. 361 (9373), pp. 1945-1951. https://doi.org/10.1016/S0140-6736(03)13583-0
Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein
Ghali, L., Wong, S., Tidman, N., Quinn, A., Philpott, M. and Leigh, I. 2004. Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein. Journal of investigative dermatology. 122 (2), pp. 433-442. https://doi.org/10.1046/j.0022-202X.2004.22207.x
FOXM1 is a downstream target of Gli1 in basal cell carcinomas
Teh, M., Wong, S., Neill, G., Ghali, L., Philpott, M. and Quinn, A. 2002. FOXM1 is a downstream target of Gli1 in basal cell carcinomas. Cancer Research. 62 (16), pp. 4773-4780.
Loss of protein kinase calpha expression may enhance the tumorigenic potential of Gli1 in basal cell carcinoma
Ghali, L., Green, J., Ikram, M. and Neill, G. 2003. Loss of protein kinase calpha expression may enhance the tumorigenic potential of Gli1 in basal cell carcinoma. Cancer Research. 63 (15), pp. 4692-4697.
Dexamethasone-induced expression of the glucocorticoid response gene lipocalin 2 in chondrocytes
Owen, H., Roberts, S., Ahmed, S. and Farquharson, C. 2008. Dexamethasone-induced expression of the glucocorticoid response gene lipocalin 2 in chondrocytes. American Journal of Physiology - Endocrinology and Metabolism. 294 (6), pp. E1023-E1034. https://doi.org/10.1152/ajpendo.00586.2007
Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1
Roberts, S., Owen, H. and Farquharson, C. 2008. Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1. Biochemical and Biophysical Research Communications. 371 (4), pp. 872-876. https://doi.org/10.1016/j.bbrc.2008.04.163
Identification and functional involvement of lipocalin 2 in glucocorticoid-induced growth retardation
Owen, H., Ahmed, S. and Farquharson, C. 2007. Identification and functional involvement of lipocalin 2 in glucocorticoid-induced growth retardation. 29th Annual Meeting of the American Society for Bone and Mineral Research. Honolulu, HI 16 - 19 Sep 2007 Wiley.
The role of P21CIP1/WAF1 in glucocorticoid induced growth retardation
Owen, H., Ahmed, S. and Farquharson, C. 2007. The role of P21CIP1/WAF1 in glucocorticoid induced growth retardation. 2007 Annual Meeting of the Bone Research Society. Southampton, UK 03 - 05 Jul 2007 Wiley.
Identification of lipocalin 2, a novel glucocorticoid responsive gene in growth plate chondrocytes
Owen, H., Ahmed, S. and Farquharson, C. 2007. Identification of lipocalin 2, a novel glucocorticoid responsive gene in growth plate chondrocytes. 2007 Annual Meeting of the Bone Research Society. Southampton, UK 03 - 05 Jul 2007 Wiley.
The growth plate sparing effects of the selective glucocorticoid receptor modulator, AL-438
Owen, H., Miner, J., Ahmed, S. and Farquharson, C. 2007. The growth plate sparing effects of the selective glucocorticoid receptor modulator, AL-438. Molecular and Cellular Endocrinology. 264 (1-2), pp. 164-70. https://doi.org/10.1016/j.mce.2006.11.006
Glucocorticoids stimulate p21 expression in growth plate chondrocytes
Owen, H., Ahmed, S. and Farquharson, C. 2006. Glucocorticoids stimulate p21 expression in growth plate chondrocytes. 2006 Annual Meeting of the Bone Research Society. Southampton, UK 05 - 06 Jul 2006 Wiley.
Glucocorticoids induce lipocalin 2 gene expression in growth plate chondrocytes.
Owen, H., Ahmed, S. and Farquharson, C. 2006. Glucocorticoids induce lipocalin 2 gene expression in growth plate chondrocytes. 28th Annual Meeting of the American Society for Bone and Mineral Research. Philadelphia, PA 15 - 19 Sep 2006 Wiley.
The role of the 11 beta HSD shuttle in modulating the effects of proinflammatory cytokines on the growth plate
MacRae, V., Owen, H., Ahmed, S. and Farquharson, C. 2006. The role of the 11 beta HSD shuttle in modulating the effects of proinflammatory cytokines on the growth plate. 28th Annual Meeting of the American Society for Bone and Mineral Research. Philadelphia, PA 15 - 19 Sep 2006 Wiley.