Granulosa cell survival and proliferation are altered in polycystic ovary syndrome

Article

Das, M., Djahanbakhch, O., Hacihanefioglu, B., Saridogan, E., Ikram, M., Ghali, L., Raveendran, M. and Storey, A. 2008. Granulosa cell survival and proliferation are altered in polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism. 93 (3), pp. 881-887. https://doi.org/10.1210/jc.2007-1650
TypeArticle
TitleGranulosa cell survival and proliferation are altered in polycystic ovary syndrome
AuthorsDas, M., Djahanbakhch, O., Hacihanefioglu, B., Saridogan, E., Ikram, M., Ghali, L., Raveendran, M. and Storey, A.
Abstract

Context: Polycystic ovary syndrome (PCOS) represents the most common endocrine abnormality in women of reproductive age. The cause of PCOS remains largely unknown, but studies suggest an intrinsic ovarian abnormality.
Objective: The objective of the study was to test our hypothesis that differences in granulosa cell proliferation and apoptosis may underlie abnormalities that affect follicular development.
Design: Granulosa cells were prepared from follicular fluid aspirated from 4- to 8-mm follicles of unstimulated ovaries during routine laparoscopy or laparotomy from women with anovulatory PCOS and those with regular ovulatory cycles.
Setting: The study was conducted at a university hospital.
Patients: Fourteen women with anovulatory PCOS and nine women with regular ovulatory cycles participated in the study.
Main Outcome Measures: Immunocytochemistry on granulosa cells to investigate apoptotic and proliferation rates, together with real-time RT-PCR to analyze gene expression profiles of apoptotic regulators, was measured.
Results: Significantly lower apoptotic rates were found in granulosa cells from patients with PCOS, compared with women with regular ovulatory cycles (P = 0.004). Lower apoptotic rates were associated with decreased levels of the apoptotic effector caspase-3 (P = 0.001) and increased levels of the anti-apoptotic survival factor cellular inhibitor of apoptosis proteins-2 in the PCOS group that were coupled to higher proliferation rates (P = 0.032). Gene expression profiling confirmed the immunocytochemical findings.
Conclusions: Our findings indicate that there are significant differences in the rate of cell death and proliferation in granulosa cell populations in PCOS patients. These are associated with decreased expression of apoptotic effectors and increased expression of a cell survival factor. These results provide new insights that may be useful in developing specific therapeutic intervention strategies in PCOS.

Research GroupBiomarkers for Cancer group
PublisherThe Endocrine Society
JournalJournal of Clinical Endocrinology and Metabolism
ISSN0021-972X
Publication dates
PrintMar 2008
Publication process dates
Deposited18 Jun 2009
Output statusPublished
Additional information

PubMed PMID: 18073308.

Web address (URL)http://jcem.endojournals.org/cgi/content/full/93/3/881
Digital Object Identifier (DOI)https://doi.org/10.1210/jc.2007-1650
LanguageEnglish
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