Modelling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

Article


Zuk, A., Wen, S., Dilworth, S., Li, D. and Ghali, L. 2017. Modelling and validating three dimensional human normal cervix and cervical cancer tissues in vitro. Journal of Biomedical Research. 31 (3), pp. 240-247. https://doi.org/10.7555/JBR.31.20160150
TypeArticle
TitleModelling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
AuthorsZuk, A., Wen, S., Dilworth, S., Li, D. and Ghali, L.
Abstract

Objective: The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-D in vitro model of normal and cancerous cervical tissue.
Methods: Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues. De-epidermised dermis (DED) was used as a scaffold for both models. Morphological analyses were conducted by using haematoxylin and eosin staining and characteristics of the models were studied by analysing the expression of different structural cytokeratins and differential protein marker Mad1 using immunohistochemical technique.
Results: Haematoxylin and eosin staining results showed that normal cervical tissue had multi epithelial layers while cancerous cervical tissue showed dysplastic changes. Immunohistochemistry staining results revealed that for normal cervix model cytokeratin 10 was expressed in the upper stratified layer of epithelium while cytokeratin 5 was expressed mainly in the middle and basal layer. Cytokeratin 19 was weakly expressed in a few basal cells. Cervical cancer model showed cytokeratin 19 expression in different epithelial layers and weak or no expression for cytokeratin 5 and cytokeratin 10. Mad1 expression was detected in some suprabasal cells.
Conclusions: The 3-D in vitro models showed stratified epithelial layers and expressed the same types and patterns of differentiation marker proteins as seen in corresponding in vivo tissue in either normal cervical or cervical cancerous tissue. Findings imply that they can serve as functional normal and cervical cancer models.

Keywordscervical cancer; MAX dimerisation protein 1; cytokeratins; three dimensional in vitro models
Research GroupBiomarkers for Cancer group
LanguageEnglish
PublisherNanjing Medical University
JournalJournal of Biomedical Research
ISSN1674-8301
Publication dates
Online20 Mar 2017
Print31 May 2017
Publication process dates
Deposited20 Sep 2017
Submitted15 Nov 2016
Accepted20 Jan 2017
Output statusPublished
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© 2017 by the Journal of Biomedical Research. All rights reserved
This is an open access article under the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited.

Web address (URL)http://www.jbr-pub.org.cn/en/article/doi/10.7555/JBR.31.20160150
Digital Object Identifier (DOI)https://doi.org/10.7555/JBR.31.20160150
Web of Science identifierWOS:000406422400008
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