Human granulosa-lutein cell in vitro production of progesterone, inhibin A, inhibin B, and activin A are dependent on follicular size and not the presence of the oocyte

Article


Wen, S., Tozer, A., Li, D., Docherty, S., Al-Shawaf, T. and Iles, R. 2008. Human granulosa-lutein cell in vitro production of progesterone, inhibin A, inhibin B, and activin A are dependent on follicular size and not the presence of the oocyte. Fertility and Sterility. 89 (5), pp. 1406-1413. https://doi.org/10.1016/j.fertnstert.2007.03.086
TypeArticle
TitleHuman granulosa-lutein cell in vitro production of progesterone, inhibin A, inhibin B, and activin A are dependent on follicular size and not the presence of the oocyte
AuthorsWen, S., Tozer, A., Li, D., Docherty, S., Al-Shawaf, T. and Iles, R.
Abstract

Objective:
To investigate inhibin A, inhibin B, activin A, and P production by cultured granulosa cells (GCs) and what relationship this hormone production has to fertility.
Design:
Luteinized GCs from individual follicles were cultured, and inhibin A, inhibin B, activin A, and P production were measured by ELISA at 24 and 72 hours.
Setting:
Research laboratory and university hospital.
Patient(s):
Fifteen women who undertook an IVF-ICSI program, yielding 58 follicles.
Intervention(s):
Individual follicular aspiration and preparation of GCs for culture.
Main Outcome Measure(s):
Inhibin A, inhibin B, activin A, and P production; oocyte retrieval; and fertility outcome.
Result(s):
Inhibin A, inhibin B, and P continued to be secreted by GCs in vitro, and activin A levels were detected only marginally in 56% of cultures. The rate of production also was dependent on the size of follicle from which the GCs originated but not on oocyte presence or ability to fertilize. Granulosa cell stimulation with hCG had no effect on inhibin A but increased P and decreased inhibin B production.
Conclusion(s):
A marked effect of luteal differentiation appears to be the inhibition of inhibin B production in response to hCG stimulation. Luteinized GC function, with respect to inhibins, activin A, and P production, was not influenced by the presence or absence of an oocyte and did not correlate with fertility outcome. However, follicle size did influence rates of local hormone production.

KeywordsProgesterone, inhibins, activin A, granulosa-lutein cells, follicular maturation
Research GroupBiomarkers for Cancer group
PublisherElsevier
JournalFertility and Sterility
ISSN0015-0282
Electronic1556-5653
Publication dates
PrintMay 2008
Publication process dates
Deposited20 May 2009
Output statusPublished
Additional information

Supplement

Digital Object Identifier (DOI)https://doi.org/10.1016/j.fertnstert.2007.03.086
Web of Science identifierWOS:000256075500016
LanguageEnglish
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