Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ

Article


Burczynska, B., Kobrouly, L., Butler, S., Naase, M. and Iles, R. 2014. Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ. Anticancer Research. 34 (5), pp. 2239-2248.
TypeArticle
TitleNovel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ
AuthorsBurczynska, B., Kobrouly, L., Butler, S., Naase, M. and Iles, R.
Abstract

BACKGROUND
Ectopic secretion of human chorionic gonadotrophin free beta (hCGβ) by epithelial cancer is associated with aggressive tumors which more readily metastasize, possibly by acting as an autocrine anti-apoptotic agent. hCGβ is encoded by six homologous CGB genes, with poorly-understood variable transcriptionally active expression profiles; CGB1 and CGB2 have always been considered pseudogenes. However, transcripts from CGB1 and -2 can be detected in placental, testicular and pituitary tissues. The expression and function of these genes in cancer is less well-known.
MATERIALS AND METHODS
Expression profiles of CGB genes in epithelial cancer cells by quantitative polymerase chain reaction (qPCR) were explored, along with the consequence of specific siRNA silencing of CGB1 and 2. Immunohistochemical and immunoassay techniques were used to detect the translation and secretion of hCGβ in these cells.
RESULTS
CGB1 and -2 gene transcripts were only detected in cells which secreted hCGβ. siRNA-mediated silencing of CGB1 and -2 transcripts significantly reduced secreted protein in concordance with a reduction in cell survival to a greater degree than that of other CGB genes.
CONCLUSION
CGB genes 1 and 2, previously considered as pseudogenes, are notably expressed by epithelial cancer cell lines. The transcription of these genes, but not other CGB genes, correlates with a functionally expressed protein and propensity for cancer growth.

Research GroupBiomarkers for Cancer group
Molecular Biology group
PublisherInternational Institute of Anticancer Research (IIAR)
JournalAnticancer Research
ISSN0250-7005
Electronic1791-7530
Publication dates
Online31 May 2014
Print31 May 2014
Publication process dates
Deposited12 Dec 2018
Accepted24 Mar 2014
Output statusPublished
Publisher's version
Copyright Statement

The attached document is the published version of an article published by International Institute of Anticancer Research (IIAR) in the journal Anticancer Research, 34 (5). pp. 2239-2248. Permission was granted, on the 01/09/2020, by IIAR to make the full text available in this repository.

Web address (URL)http://ar.iiarjournals.org/content/34/5/2239.long
LanguageEnglish
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