Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ

Article


Burczynska, B., Kobrouly, L., Butler, S., Naase, M. and Iles, R. 2014. Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ. Anticancer Research. 34 (5), pp. 2239-2248.
TypeArticle
TitleNovel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ
AuthorsBurczynska, B., Kobrouly, L., Butler, S., Naase, M. and Iles, R.
Abstract

BACKGROUND
Ectopic secretion of human chorionic gonadotrophin free beta (hCGβ) by epithelial cancer is associated with aggressive tumors which more readily metastasize, possibly by acting as an autocrine anti-apoptotic agent. hCGβ is encoded by six homologous CGB genes, with poorly-understood variable transcriptionally active expression profiles; CGB1 and CGB2 have always been considered pseudogenes. However, transcripts from CGB1 and -2 can be detected in placental, testicular and pituitary tissues. The expression and function of these genes in cancer is less well-known.
MATERIALS AND METHODS
Expression profiles of CGB genes in epithelial cancer cells by quantitative polymerase chain reaction (qPCR) were explored, along with the consequence of specific siRNA silencing of CGB1 and 2. Immunohistochemical and immunoassay techniques were used to detect the translation and secretion of hCGβ in these cells.
RESULTS
CGB1 and -2 gene transcripts were only detected in cells which secreted hCGβ. siRNA-mediated silencing of CGB1 and -2 transcripts significantly reduced secreted protein in concordance with a reduction in cell survival to a greater degree than that of other CGB genes.
CONCLUSION
CGB genes 1 and 2, previously considered as pseudogenes, are notably expressed by epithelial cancer cell lines. The transcription of these genes, but not other CGB genes, correlates with a functionally expressed protein and propensity for cancer growth.

Research GroupBiomarkers for Cancer group
Molecular Biology group
LanguageEnglish
PublisherInternational Institute of Anticancer Research (IIAR)
JournalAnticancer Research
ISSN0250-7005
Electronic1791-7530
Publication dates
Online31 May 2014
Print31 May 2014
Publication process dates
Deposited12 Dec 2018
Accepted24 Mar 2014
Output statusPublished
Publisher's version
Copyright Statement

The attached document is the published version of an article published by International Institute of Anticancer Research (IIAR) in the journal Anticancer Research, 34 (5). pp. 2239-2248. Permission was granted, on the 01/09/2020, by IIAR to make the full text available in this repository.

Web address (URL)http://ar.iiarjournals.org/content/34/5/2239.long
Permalink -

https://repository.mdx.ac.uk/item/8813x

  • 24
    total views
  • 7
    total downloads
  • 1
    views this month
  • 3
    downloads this month

Export as

Related outputs

Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year
Hassard, F., Vu, M., Rahimzadeh, S., Castro-Gutierrez, V., Stanton, I., Burczynska, B., Wildeboer, D., Baio, G., Brown, M., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Majeed, A., Priest, S., Denise, H., Khalifa, M., Bassano, I., Wade, M., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2023. Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year. PLoS ONE. 18 (5). https://doi.org/10.1371/journal.pone.0286259
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. 2018 NCRI Cancer Conference. Glasgow, Scotland 04 - 06 Nov 2018
Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach
Castro-Gutierrez, V., Hassard, F., Vu, M., Leitao, R., Burczynska, B., Wildeboer, D., Stanton, I., Rahimzadeh, S., Baio, G., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Kwiatkowska, R., Majeed, A., Priest, S., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2022. Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach. PLoS ONE. 17 (6). https://doi.org/10.1371/journal.pone.0270168
Geographical and socioeconomic inequalities in female breast cancer incidence and mortality in Iran: A Bayesian spatial analysis of registry data
Rahimzadeh, S., Burczynska, B., Ahmadvand, A., Sheidaei, A., Khademioureh, S., Pazhuheian, F., Saeedi Moghaddam, S., Bentham, J., Farzadfar, F. and Di Cesare, M. 2021. Geographical and socioeconomic inequalities in female breast cancer incidence and mortality in Iran: A Bayesian spatial analysis of registry data. PLoS ONE. 16 (3), pp. 1-19. https://doi.org/10.1371/journal.pone.0248723
Modelling and validating three-dimensional human breast and cancerous human breast tissues in vitro
Zuk, A., Burczynska, B., Li, D., Ghali, L., Dilworth, S. and Wen, S. 2020. Modelling and validating three-dimensional human breast and cancerous human breast tissues in vitro. Clinical Oncology and Research. 3 (4), pp. 1-9. https://doi.org/10.31487/j.COR.2020.04.05
Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS
Gondek, M., Burczynska, B. and Hills, F. 2019. Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS. 4th FEBS Congress. Krakow, Poland 06 - 11 Jul 2019 Wiley. https://doi.org/10.1002/2211-5463.12674
Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer
Śliwa, A., Kubiczak, M., Szczerba, A., Walkowiak, G., Nowak-Markwitz, E., Burczynska, B., Butler, S., Iles, R., Białas, P. and Jankowska, A. 2019. Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer. BMC cancer. 19 (1), pp. 1-9. https://doi.org/10.1186/s12885-019-5960-2
Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers
Gondek, M., Hills, F. and Burczynska, B. 2015. Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers. Summer Conference. Middlesex University, London, United Kingdom Jun 2015 Middlesex University.
Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS
Gondek, M., Burczynska, B. and Hills, F. 2018. Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS. 45th International Society of Oncology and BioMarkers (ISOBM) 2018 Congress. Hamburg, Germany 24 - 27 Nov 2018
Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines
Gondek, M., Hills, F. and Burczynska, B. 2017. Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines. Summer Conference 2017. Middlesex University Jun 2017
A comparative study of bifidobacteria in human babies and adults
Khonsari, S., Suganthy, M., Burczynska, B., Dang, V., Choudhury, M. and Pachenari, A. 2016. A comparative study of bifidobacteria in human babies and adults. Bioscience of Microbiota, Food and Health. 35 (2), pp. 97-103. https://doi.org/10.12938/bmfh.2015-006
Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells
Jankowska, A., Wiecek, P. and Burczynska, B. 2008. Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells. Photomedicine and Laser Surgery. 26 (4), pp. 343-347. https://doi.org/10.1089/pho.2007.2199
Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth
Burczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S. 2013. Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth. Anticancer Research. 33 (9), pp. 3611-3614.
Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm
Jankowska, A., Burczynska, B., Duda, T. and Warchol, J. 2010. Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm. Fertility and Sterility. 93 (3), pp. 904-912. https://doi.org/10.1016/j.fertnstert.2008.10.048
Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells
Jankowska, A., Gunderson, S., Andrusiewicz, M., Burczynska, B., Szczerba, A., Jarmolowski, A., Nowak-Markwitz, E. and Warchol, J. 2008. Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells. Molecular Cancer. 7 (26). https://doi.org/10.1186/1476-4598-7-26