Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells

Article


Jankowska, A., Wiecek, P. and Burczynska, B. 2008. Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells. Photomedicine and Laser Surgery. 26 (4), pp. 343-347. https://doi.org/10.1089/pho.2007.2199
TypeArticle
TitleEffect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells
AuthorsJankowska, A., Wiecek, P. and Burczynska, B.
Abstract

Objective: The aim of the study was to test the effect of photodynamic therapy on fibroblast proliferation in vitro using protoporphyrin IX as a photosensitizer.
Background Data: The abnormal proliferation of synovial tissue serves as a propagator of immune response and tissue damage in rheumatoid arthritis. Since the synovial fibroblasts mediate most relevant pathways of joint destruction, they constitute an important target for novel therapeutic approaches. Photodynamic therapy, which is the clinically applied treatment for various tumors, as well as for some non-oncologic diseases, is based on administration of an exogenous photosensitizer which is used to induce cell death of fibroblast cells.
Materials and Methods: The photosensitizing effects of protoporphyrin IX (PpIX) were studied in the 3T3 cell line that served as a model of fibroblasts and HeLa cells cultured in vitro.
Results: The number of fibroblasts and HeLa cells treated with photo-activated PpIX decreased significantly. The compound affects the viability of study cells, causing necrosis of 3T3 cells and apoptosis of HeLa cells.
Conclusions: The light-activated protoporphyrin affected proliferation of both 3T3 and HeLa cells. No effects of the phototherapy were seen in the form of apoptosis of 3T3 cells, whereas the induction of cell death in HeLa cells was detected.

Research GroupBiomarkers for Cancer group
LanguageEnglish
PublisherMary Ann Liebert
JournalPhotomedicine and Laser Surgery
ISSN1549-5418
Publication dates
Print2008
Publication process dates
Deposited07 Nov 2013
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1089/pho.2007.2199
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