Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells

Article

Jankowska, A., Wiecek, P. and Burczynska, B. 2008. Effect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells. Photomedicine and Laser Surgery. 26 (4), pp. 343-347. https://doi.org/10.1089/pho.2007.2199
TypeArticle
TitleEffect of photodynamic therapy on proliferation and apoptosis of 3T3 fibroblasts and HeLa cells
AuthorsJankowska, A., Wiecek, P. and Burczynska, B.
Abstract

Objective: The aim of the study was to test the effect of photodynamic therapy on fibroblast proliferation in vitro using protoporphyrin IX as a photosensitizer.
Background Data: The abnormal proliferation of synovial tissue serves as a propagator of immune response and tissue damage in rheumatoid arthritis. Since the synovial fibroblasts mediate most relevant pathways of joint destruction, they constitute an important target for novel therapeutic approaches. Photodynamic therapy, which is the clinically applied treatment for various tumors, as well as for some non-oncologic diseases, is based on administration of an exogenous photosensitizer which is used to induce cell death of fibroblast cells.
Materials and Methods: The photosensitizing effects of protoporphyrin IX (PpIX) were studied in the 3T3 cell line that served as a model of fibroblasts and HeLa cells cultured in vitro.
Results: The number of fibroblasts and HeLa cells treated with photo-activated PpIX decreased significantly. The compound affects the viability of study cells, causing necrosis of 3T3 cells and apoptosis of HeLa cells.
Conclusions: The light-activated protoporphyrin affected proliferation of both 3T3 and HeLa cells. No effects of the phototherapy were seen in the form of apoptosis of 3T3 cells, whereas the induction of cell death in HeLa cells was detected.

Research GroupBiomarkers for Cancer group
PublisherMary Ann Liebert
JournalPhotomedicine and Laser Surgery
ISSN1549-5418
Publication dates
Print2008
Publication process dates
Deposited07 Nov 2013
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1089/pho.2007.2199
LanguageEnglish
Permalink -

https://repository.mdx.ac.uk/item/8484q

Log in to edit
  • 57
    total views
  • 0
    total downloads
  • 3
    views this month
  • 0
    downloads this month

Export as

Related outputs

Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year
Hassard, F., Vu, M., Rahimzadeh, S., Castro-Gutierrez, V., Stanton, I., Burczynska, B., Wildeboer, D., Baio, G., Brown, M., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Majeed, A., Priest, S., Denise, H., Khalifa, M., Bassano, I., Wade, M., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2023. Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year. PLoS ONE. 18 (5). https://doi.org/10.1371/journal.pone.0286259
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. Research Degrees Students' Summer Conference. Middlesex University, London, UK 04 Sep 2018
Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
Rosa, G., Burczynska, B., Stordal, B. and Roberts, H. 2018. Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy. 2018 NCRI Cancer Conference. Glasgow, Scotland 04 - 06 Nov 2018
Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach
Castro-Gutierrez, V., Hassard, F., Vu, M., Leitao, R., Burczynska, B., Wildeboer, D., Stanton, I., Rahimzadeh, S., Baio, G., Garelick, H., Hofman, J., Kasprzyk-Hordern, B., Kwiatkowska, R., Majeed, A., Priest, S., Grimsley, J., Lundy, L., Singer, A. and Di Cesare, M. 2022. Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach. PLoS ONE. 17 (6). https://doi.org/10.1371/journal.pone.0270168
Geographical and socioeconomic inequalities in female breast cancer incidence and mortality in Iran: A Bayesian spatial analysis of registry data
Rahimzadeh, S., Burczynska, B., Ahmadvand, A., Sheidaei, A., Khademioureh, S., Pazhuheian, F., Saeedi Moghaddam, S., Bentham, J., Farzadfar, F. and Di Cesare, M. 2021. Geographical and socioeconomic inequalities in female breast cancer incidence and mortality in Iran: A Bayesian spatial analysis of registry data. PLoS ONE. 16 (3), pp. 1-19. https://doi.org/10.1371/journal.pone.0248723
Modelling and validating three-dimensional human breast and cancerous human breast tissues in vitro
Zuk, A., Burczynska, B., Li, D., Ghali, L., Dilworth, S. and Wen, S. 2020. Modelling and validating three-dimensional human breast and cancerous human breast tissues in vitro. Clinical Oncology and Research. 3 (4), pp. 1-9. https://doi.org/10.31487/j.COR.2020.04.05
Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS
Gondek, M., Burczynska, B. and Hills, F. 2019. Purification and method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI IT–TOF MS. 4th FEBS Congress. Krakow, Poland 06 - 11 Jul 2019 Wiley. https://doi.org/10.1002/2211-5463.12674
Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer
Śliwa, A., Kubiczak, M., Szczerba, A., Walkowiak, G., Nowak-Markwitz, E., Burczynska, B., Butler, S., Iles, R., Białas, P. and Jankowska, A. 2019. Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer. BMC cancer. 19 (1), pp. 1-9. https://doi.org/10.1186/s12885-019-5960-2
Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ
Burczynska, B., Kobrouly, L., Butler, S., Naase, M. and Iles, R. 2014. Novel insights into the expression of CGB1 & 2 genes by epithelial cancer cell lines secreting ectopic free hCGβ. Anticancer Research. 34 (5), pp. 2239-2248.
Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers
Gondek, M., Hills, F. and Burczynska, B. 2015. Can you spot the difference? Analysis of human chorionic gonadotropin (hCG) secreted by common non-trophoblastic and trophoblastic cancers. Summer Conference. Middlesex University, London, United Kingdom Jun 2015 Middlesex University.
Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS
Gondek, M., Burczynska, B. and Hills, F. 2018. Method optimisation for identification of carbohydrate moieties on human chorionic gonadotropin glycoforms using MALDI-TOF-MS. 45th International Society of Oncology and BioMarkers (ISOBM) 2018 Congress. Hamburg, Germany 24 - 27 Nov 2018
Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines
Gondek, M., Hills, F. and Burczynska, B. 2017. Detection and quantification of human chorionic gonadotropin levels in human placental choriocarcinoma cell lines. Summer Conference 2017. Middlesex University Jun 2017
A comparative study of bifidobacteria in human babies and adults
Khonsari, S., Suganthy, M., Burczynska, B., Dang, V., Choudhury, M. and Pachenari, A. 2016. A comparative study of bifidobacteria in human babies and adults. Bioscience of Microbiota, Food and Health. 35 (2), pp. 97-103. https://doi.org/10.12938/bmfh.2015-006
Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth
Burczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S. 2013. Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth. Anticancer Research. 33 (9), pp. 3611-3614.
Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells
Jankowska, A., Gunderson, S., Andrusiewicz, M., Burczynska, B., Szczerba, A., Jarmolowski, A., Nowak-Markwitz, E. and Warchol, J. 2008. Reduction of human chorionic gonadotropin beta subunit expression by modified U1 snRNA caused apoptosis in cervical cancer cells. Molecular Cancer. 7 (26). https://doi.org/10.1186/1476-4598-7-26
Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm
Jankowska, A., Burczynska, B., Duda, T. and Warchol, J. 2010. Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm. Fertility and Sterility. 93 (3), pp. 904-912. https://doi.org/10.1016/j.fertnstert.2008.10.048