Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth

Article


Burczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S. 2013. Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth. Anticancer Research. 33 (9), pp. 3611-3614.
TypeArticle
TitleStable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth
AuthorsBurczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S.
Abstract

Background: Expression of human chorionic gonadotropin beta subunit (hCGβ) by epithelial carcinomas is associated with a poor prognosis and has a proposed autocrine growth effect on cancer cells by inhibition of apoptosis. Material and Methods: We transduced the hCGβ-expressing bladder cancer cell line SCaBER with short hairpin (sh) RNA lentiviral gene-specific (CGB) constructs and determined its impact on the synthesis of hCGβ and the resultant effect on cancer cell growth. Results: Stable CGB gene-silenced clones exhibited a 60%-80% reduction in the level of hCGβ expressed and a reduced growth rate of more than 40% compared to wild-type SCaBER cells. Conclusions: shRNA Lentiviral particles achieve stable knockdown of hCGβ translation in the bladder cancer cell line SCaBER. This transforms the phenotype by reducing hCGβ expression and cell growth rate. This is consistent with the proposed autocrine/paracrine function of ectopic hCGβ expression during oncogenesis.

Research GroupBiomarkers for Cancer group
LanguageEnglish
PublisherInternational Institute of Anticancer Research (IIAR)
JournalAnticancer Research
ISSN0250-7005
Electronic1791-7530
Publication dates
Print30 Sep 2013
Publication process dates
Deposited18 Sep 2013
Accepted17 Jul 2013
Output statusPublished
Web address (URL)http://ar.iiarjournals.org/content/33/9/3611.abstract
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