Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth
Article
Burczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S. 2013. Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth. Anticancer Research. 33 (9), pp. 3611-3614.
Type | Article |
---|---|
Title | Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth |
Authors | Burczynska, B., Booth, M., Iles, R., Shah, A., Shiled, A. and Butler, S. |
Abstract | Background: Expression of human chorionic gonadotropin beta subunit (hCGβ) by epithelial carcinomas is associated with a poor prognosis and has a proposed autocrine growth effect on cancer cells by inhibition of apoptosis. Material and Methods: We transduced the hCGβ-expressing bladder cancer cell line SCaBER with short hairpin (sh) RNA lentiviral gene-specific (CGB) constructs and determined its impact on the synthesis of hCGβ and the resultant effect on cancer cell growth. Results: Stable CGB gene-silenced clones exhibited a 60%-80% reduction in the level of hCGβ expressed and a reduced growth rate of more than 40% compared to wild-type SCaBER cells. Conclusions: shRNA Lentiviral particles achieve stable knockdown of hCGβ translation in the bladder cancer cell line SCaBER. This transforms the phenotype by reducing hCGβ expression and cell growth rate. This is consistent with the proposed autocrine/paracrine function of ectopic hCGβ expression during oncogenesis. |
Research Group | Biomarkers for Cancer group |
Publisher | International Institute of Anticancer Research (IIAR) |
Journal | Anticancer Research |
ISSN | 0250-7005 |
Electronic | 1791-7530 |
Publication dates | |
30 Sep 2013 | |
Publication process dates | |
Deposited | 18 Sep 2013 |
Accepted | 17 Jul 2013 |
Output status | Published |
Web address (URL) | http://ar.iiarjournals.org/content/33/9/3611.abstract |
Language | English |
https://repository.mdx.ac.uk/item/843yy
56
total views0
total downloads0
views this month0
downloads this month