Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm

Article


Jankowska, A., Burczynska, B., Duda, T. and Warchol, J. 2010. Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm. Fertility and Sterility. 93 (3), pp. 904-912. https://doi.org/10.1016/j.fertnstert.2008.10.048
TypeArticle
TitleRod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm
AuthorsJankowska, A., Burczynska, B., Duda, T. and Warchol, J.
Abstract

OBJECTIVE:
Evaluation of the presence of a Ca(2+)-regulated membrane guanylate cyclase signal transudation system in the spermatozoa.
DESIGN:
Experimental study.
SETTING:
Research university laboratory.
PATIENT(S):
Human sperm obtained from healthy donors who met the criteria of the World Health Organization for normozoospermia and bovine semen collected from bulls of proven fertility.
INTERVENTION(S):
Radioimmunoassay and immunohistochemistry of human and bovine spermatozoa.
MAIN OUTCOME MEASURE(S):
The membrane guanylate cyclase activity and the presence of membrane guanylate cyclase transduction machinery components in the spermatozoa.
RESULT(S):
The identity of a Ca(2+)-modulated membrane guanylate cyclase transduction machinery in human and bovine spermatozoa has been documented. The machinery is both inhibited and stimulated within nanomolar to semimicromolar range of free Ca(2+). The transduction component of this machinery is the rod outer segment membrane guanylate cyclase type 1 (ROS-GC1). The enzyme coexists with three Ca(2+)-dependent modulators: guanylate cyclase activating protein type 1 (GCAP1), S100B and neurocalcin delta. ROS-GC1 and its modulators are present in the heads and tails of both species' spermatozoa.
CONCLUSION(S):
The coexpression of ROS-GC1 and its activators in spermatozoa suggests that the Ca(2+)-modulated ROS-GC1 transduction system may be a part of the fertilization machinery.

Keywordsspermatozoa, cyclic GMP, membrane guanylate cyclase, ROS-GC1, calcium, GCAP1, S100B, neurocalcin δ
Research GroupBiomarkers for Cancer group
LanguageEnglish
PublisherElsevier
JournalFertility and Sterility
ISSN0015-0282
Publication dates
PrintFeb 2010
Publication process dates
Deposited09 Jul 2013
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/j.fertnstert.2008.10.048
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