Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines
Article
Busschots, S., O'Toole, S., O'Leary, J. and Stordal, B. 2015. Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines. MethodsX. 2, pp. 8-13. https://doi.org/10.1016/j.mex.2014.11.002
Type | Article |
---|---|
Title | Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines |
Authors | Busschots, S., O'Toole, S., O'Leary, J. and Stordal, B. |
Abstract | Many protocols used for measuring the growth of adherent monolayer cells in vitro are invasive, destructive and do not allow for the continued, undisturbed growth of cells within flasks. Protocols often use indirect methods for measuring proliferation. Microscopy techniques can analyse cell proliferation in a non-invasive or non-destructive manner but often use expensive equipment and software algorithms. In this method images of cells within flasks are captured by photographing under a standard inverted phase contract light microscope using a digital camera with a camera lens adaptor. Images are analysed for confluence using ImageJ freeware resulting in a measure of confluence known as an Area Fraction (AF) output. An example of the AF method in use on OVCAR8 and UPN251 cell lines is included. |
Keywords | Confluence; Cell count; Phase-contrast microscopy; ImageJ; Area fraction; Non-destructive |
Research Group | Biomarkers for Cancer group |
Publisher | Elsevier |
Journal | MethodsX |
ISSN | |
Electronic | 2215-0161 |
Publication dates | |
Online | 25 Nov 2014 |
2015 | |
Publication process dates | |
Deposited | 08 Jan 2016 |
Accepted | 14 Nov 2014 |
Output status | Published |
Publisher's version | License |
Copyright Statement | © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). |
Web address (URL) | https://www.sciencedirect.com/science/article/pii/S2215016114000260 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.mex.2014.11.002 |
PubMed ID | 26150966 |
PubMed Central ID | 4487325 |
Web of Science identifier | WOS:000370471500002 |
Language | English |
https://repository.mdx.ac.uk/item/86190
Download files
58
total views10
total downloads0
views this month0
downloads this month