High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates

Article

Hows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates. Journal of Neuroscience Methods. 137 (2), pp. 221-226. https://doi.org/10.1016/j.jneumeth.2004.02.011
TypeArticle
TitleHigh-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
AuthorsHows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A.
Abstract

A high-throughput liquid chromatography/tandem mass spectrometry method has been developed for the quantitative assessment of 1-methyl-4-phenylpyridinium (MPP+) in brain tissue samples. This separation is based on reversed phase chromatography using formic acid and acetonitrile as the mobile phase. Using gradient separation conditions, MPP+ was resolved within 5 min and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for MPP+ was found to be 1 fmol on column with a signal to noise ratio of 3:1. The assay has been used routinely in our laboratory for the measurement of MPP+ levels in brain tissue from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, and can be used to distinguish neuroprotective efficacy and monoamine oxidase inhibition.

LanguageEnglish
PublisherElsevier
JournalJournal of Neuroscience Methods
ISSN0165-0270
Publication dates
Online17 Apr 2004
Print30 Aug 2004
Publication process dates
Deposited03 Nov 2017
Accepted18 Feb 2004
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jneumeth.2004.02.011
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