High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
Article
Hows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A. 2004. High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates. Journal of Neuroscience Methods. 137 (2), pp. 221-226. https://doi.org/10.1016/j.jneumeth.2004.02.011
Type | Article |
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Title | High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates |
Authors | Hows, M., Ashmeade, T., Billinton, A., Perren, M., Austin, A., Virley, D., Organ, A. and Shah, A. |
Abstract | A high-throughput liquid chromatography/tandem mass spectrometry method has been developed for the quantitative assessment of 1-methyl-4-phenylpyridinium (MPP+) in brain tissue samples. This separation is based on reversed phase chromatography using formic acid and acetonitrile as the mobile phase. Using gradient separation conditions, MPP+ was resolved within 5 min and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for MPP+ was found to be 1 fmol on column with a signal to noise ratio of 3:1. The assay has been used routinely in our laboratory for the measurement of MPP+ levels in brain tissue from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, and can be used to distinguish neuroprotective efficacy and monoamine oxidase inhibition. |
Publisher | Elsevier |
Journal | Journal of Neuroscience Methods |
ISSN | 0165-0270 |
Publication dates | |
Online | 17 Apr 2004 |
30 Aug 2004 | |
Publication process dates | |
Deposited | 03 Nov 2017 |
Accepted | 18 Feb 2004 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jneumeth.2004.02.011 |
Language | English |
https://repository.mdx.ac.uk/item/8744y
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